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Routine Tisse Processing

Routine Tisse Processing
57問 • 1年前
  • ユーザ名非公開
    • 通報

    問題一覧

  • 1

    What is the other term for infiltration?

    impregnation

  • 2

    Removal of EXCESS PARAFFIN WAX

    trimming

  • 3

    Its reason is to expose tissue in preparation for section cutting

    trimming

  • 4

    What is the thickness of the wax surrounding the tissue?

    2mm

  • 5

    Cutting of tissue block into uniformly thin slices

    section cutting

  • 6

    Tissue ribbon is made in?

    section cutting

  • 7

    Technicians needs training to operate

    section cutting

  • 8

    The nearer the knife, the thicker the tissue ribbon

    false (thinner)

  • 9

    What are the three parts of the microtome

    knife carrier, knife, pawl (ratchet feed wheel and adjustment screws)

  • 10

    Cutting the serial section of large blocks of paraffin embedded tissues (note: large spemine only)

    rocking microtome

  • 11

    Cutting paraffin embedded section

    rotary (minot) microtome

  • 12

    Cutting celloidin embedded section

    sliding microtome

  • 13

    Cutting unembedded frozen sections

    freezing microtome

  • 14

    Cutting frozen sections: urgent biopsies and must be scheduled at least a day prior

    cryostat (cold) microtome

  • 15

    Used during surgery: benign or malignant, determines if extensive surgery is needed

    freezing microtome

  • 16

    What is the optimal temperature in cryostat (cold) microtome?

    -20 celsius

  • 17

    What is the cryostat temperature in cryostat (cold) microtome?

    -5 celsius to -30 celsius within 2 to 3 minutes

  • 18

    Cutting sections for EM. Very large

    ultrathin microtome

  • 19

    All accumulated paraffin and small pieces of tissue MUST NOT be clean by BRUSH/SOFT BRUSH

    false (MUST BE CLEAN)

  • 20

    Movable portions should be OILED properly

    true

  • 21

    Microtome must NOT BE COVERED when not in use

    false (must be COVERED)

  • 22

    Always REMOVE BLADE/KNIFE before cleaning.

    true

  • 23

    Have the instrumemt inspected by a technician at least ONCE A MONTH (for calibration purposes)

    false (ONCE A YEAR)

  • 24

    Removal of GROSS NICKS

    honing

  • 25

    Edge first: HEEL TO TOE

    honing

  • 26

    Removal of BURR

    stropping

  • 27

    Edge first: TOE TO HEEL

    stropping

  • 28

    Purpose of this is to flatten the tissue sections with temperature of 6-10 degree celsius below melting point)

    flotation bath

  • 29

    tissue must be in the middle

    fishing out

  • 30

    Procedure: immerse slide in xylol/xylene, alcohol, water

    deparaffinization

  • 31

    Special type of test in histopath lab to DIAGNOSE CANCER. Reagents need to react with Ag/Ab to produce a color

    immunohistochemical staining

  • 32

    What are the three major groups in staining?

    histological staining, histochemical staining, immunohistochemical staining

  • 33

    Mordant (indirect staining)

    BRIDGE or LINK in between tissue and stain

  • 34

    Speeds up chemical reaction

    accentuator (indirect staining)

  • 35

    Gradual staining

    progressive

  • 36

    Overstain the decolorize

    regressive

  • 37

    Different color imparted

    metachromatic

  • 38

    Same color imparted

    orthochromatic

  • 39

    Injected on tissue

    intravital (vital staining)

  • 40

    Immediately applied after tissue is removed from the body

    supravital (vital staining)

  • 41

    Hemotoxylin dye, cochineal (carmine) dye, orcein (flower), saffron

    natural dyes

  • 42

    Coal tar

    synthetic dyes

  • 43

    Attracted to basic structures (red-pink)

    acid

  • 44

    Attracted to acidic structures (blue-purple)

    basic

  • 45

    Attracted to both dyes

    neutral

  • 46

    what is the primary stain in H&E

    Hematoxylin

  • 47

    What is the decolorizer in H&E?

    acid alcohol

  • 48

    What is the blueing agent in H&E?

    ammonia water

  • 49

    What is the secondary stain in H&E?

    eosin

  • 50

    What is the dehydrator in H&E?

    ascending grades of alcohol

  • 51

    Xylene —> mounting —> labelling

    true

  • 52

    A medium applied BETWEEN tissue slide and cover slip

    true

  • 53

    A medium applied between tissue slide and cover slip

    mounting

  • 54

    Refractive index (RI) should be near to RI of glass (1.1516) (should have no bubbles)

    false (1.158)

  • 55

    Sealing the edge of the cover slip

    ringing

  • 56

    Not routinely done

    ringing

  • 57

    Uses pencil on the frosted end of the slide

    labelling

    • airlaw and airworthyness

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    ユーザ名非公開 · 12問 · 2年前

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    12問 • 2年前
    ユーザ名非公開

    maintenance forms and records

    maintenance forms and records

    ユーザ名非公開 · 36問 · 2年前

    maintenance forms and records

    maintenance forms and records

    36問 • 2年前
    ユーザ名非公開

    maintenance publications

    maintenance publications

    ユーザ名非公開 · 38問 · 2年前

    maintenance publications

    maintenance publications

    38問 • 2年前
    ユーザ名非公開

    mechanic privileges and limitations

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    ユーザ名非公開 · 33問 · 2年前

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    33問 • 2年前
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    pcar

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    48問 • 2年前
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    AFCAR

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    ユーザ名非公開 · 60問 · 2年前

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    60問 • 2年前
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    ユーザ名非公開 · 96問 · 1年前

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    96問 • 1年前
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    ユーザ名非公開 · 22問 · 1年前

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    22問 • 1年前
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    overview of PCR

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    ユーザ名非公開 · 36問 · 1年前

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    36問 • 1年前
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    Real time PCR or Quantitative PCR (qPCR)

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    ユーザ名非公開 · 23問 · 1年前

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    23問 • 1年前
    ユーザ名非公開

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    ユーザ名非公開 · 26問 · 1年前

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    protein analysis

    26問 • 1年前
    ユーザ名非公開

    MECHANIC PRIVILEGES AND LIMITATIONS

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    ユーザ名非公開 · 34問 · 1年前

    MECHANIC PRIVILEGES AND LIMITATIONS

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    34問 • 1年前
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    CELL BLOCK

    CELL BLOCK

    ユーザ名非公開 · 22問 · 1年前

    CELL BLOCK

    CELL BLOCK

    22問 • 1年前
    ユーザ名非公開

    CELL BLOCK

    CELL BLOCK

    ユーザ名非公開 · 22問 · 1年前

    CELL BLOCK

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    22問 • 1年前
    ユーザ名非公開

    AUTOPSY

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    ユーザ名非公開 · 50問 · 1年前

    AUTOPSY

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    50問 • 1年前
    ユーザ名非公開

    FILIPINO VOCABULARY

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    ユーザ名非公開 · 11問 · 1年前

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    11問 • 1年前
    ユーザ名非公開

    WEEK 3 MACROSCOPIC, DISEASE CORRELATION, URINE COLOR, URINE VOLUME, URINE TRANSPARENCY, AND URINE ODOR

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    ユーザ名非公開 · 52問 · 1年前

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    52問 • 1年前
    ユーザ名非公開

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    ユーザ名非公開 · 31問 · 1年前

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    31問 • 1年前
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    CARBS

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    ユーザ名非公開 · 6問 · 1年前

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    6問 • 1年前
    ユーザ名非公開

    問題一覧

  • 1

    What is the other term for infiltration?

    impregnation

  • 2

    Removal of EXCESS PARAFFIN WAX

    trimming

  • 3

    Its reason is to expose tissue in preparation for section cutting

    trimming

  • 4

    What is the thickness of the wax surrounding the tissue?

    2mm

  • 5

    Cutting of tissue block into uniformly thin slices

    section cutting

  • 6

    Tissue ribbon is made in?

    section cutting

  • 7

    Technicians needs training to operate

    section cutting

  • 8

    The nearer the knife, the thicker the tissue ribbon

    false (thinner)

  • 9

    What are the three parts of the microtome

    knife carrier, knife, pawl (ratchet feed wheel and adjustment screws)

  • 10

    Cutting the serial section of large blocks of paraffin embedded tissues (note: large spemine only)

    rocking microtome

  • 11

    Cutting paraffin embedded section

    rotary (minot) microtome

  • 12

    Cutting celloidin embedded section

    sliding microtome

  • 13

    Cutting unembedded frozen sections

    freezing microtome

  • 14

    Cutting frozen sections: urgent biopsies and must be scheduled at least a day prior

    cryostat (cold) microtome

  • 15

    Used during surgery: benign or malignant, determines if extensive surgery is needed

    freezing microtome

  • 16

    What is the optimal temperature in cryostat (cold) microtome?

    -20 celsius

  • 17

    What is the cryostat temperature in cryostat (cold) microtome?

    -5 celsius to -30 celsius within 2 to 3 minutes

  • 18

    Cutting sections for EM. Very large

    ultrathin microtome

  • 19

    All accumulated paraffin and small pieces of tissue MUST NOT be clean by BRUSH/SOFT BRUSH

    false (MUST BE CLEAN)

  • 20

    Movable portions should be OILED properly

    true

  • 21

    Microtome must NOT BE COVERED when not in use

    false (must be COVERED)

  • 22

    Always REMOVE BLADE/KNIFE before cleaning.

    true

  • 23

    Have the instrumemt inspected by a technician at least ONCE A MONTH (for calibration purposes)

    false (ONCE A YEAR)

  • 24

    Removal of GROSS NICKS

    honing

  • 25

    Edge first: HEEL TO TOE

    honing

  • 26

    Removal of BURR

    stropping

  • 27

    Edge first: TOE TO HEEL

    stropping

  • 28

    Purpose of this is to flatten the tissue sections with temperature of 6-10 degree celsius below melting point)

    flotation bath

  • 29

    tissue must be in the middle

    fishing out

  • 30

    Procedure: immerse slide in xylol/xylene, alcohol, water

    deparaffinization

  • 31

    Special type of test in histopath lab to DIAGNOSE CANCER. Reagents need to react with Ag/Ab to produce a color

    immunohistochemical staining

  • 32

    What are the three major groups in staining?

    histological staining, histochemical staining, immunohistochemical staining

  • 33

    Mordant (indirect staining)

    BRIDGE or LINK in between tissue and stain

  • 34

    Speeds up chemical reaction

    accentuator (indirect staining)

  • 35

    Gradual staining

    progressive

  • 36

    Overstain the decolorize

    regressive

  • 37

    Different color imparted

    metachromatic

  • 38

    Same color imparted

    orthochromatic

  • 39

    Injected on tissue

    intravital (vital staining)

  • 40

    Immediately applied after tissue is removed from the body

    supravital (vital staining)

  • 41

    Hemotoxylin dye, cochineal (carmine) dye, orcein (flower), saffron

    natural dyes

  • 42

    Coal tar

    synthetic dyes

  • 43

    Attracted to basic structures (red-pink)

    acid

  • 44

    Attracted to acidic structures (blue-purple)

    basic

  • 45

    Attracted to both dyes

    neutral

  • 46

    what is the primary stain in H&E

    Hematoxylin

  • 47

    What is the decolorizer in H&E?

    acid alcohol

  • 48

    What is the blueing agent in H&E?

    ammonia water

  • 49

    What is the secondary stain in H&E?

    eosin

  • 50

    What is the dehydrator in H&E?

    ascending grades of alcohol

  • 51

    Xylene —> mounting —> labelling

    true

  • 52

    A medium applied BETWEEN tissue slide and cover slip

    true

  • 53

    A medium applied between tissue slide and cover slip

    mounting

  • 54

    Refractive index (RI) should be near to RI of glass (1.1516) (should have no bubbles)

    false (1.158)

  • 55

    Sealing the edge of the cover slip

    ringing

  • 56

    Not routinely done

    ringing

  • 57

    Uses pencil on the frosted end of the slide

    labelling