molecular biology

50問 • 1年前

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通報

問題一覧

1.How many volumes of ethanol are added to1volume of DNA: salt soln to cause the DNA to precipitate?

2. What type of short read files with quality scores are used to storing next generation sequencing read data?

FASTQ

3.W/c is considered an epigenetic modification of DNA?

methylation of CpG islands in the promoter region of gene

4. What term best describes the relatedness of sequence, the percent identity or conservation?

similarity

5. What type of enzyme can be used to cut a chromosome into smaller fragments of DNA w/o removing any nucleotides or functional groups?

endonuclease

6. The results of STR typing of a child,the child's mom & 3 alleged dads(AF)of the child are listed in the table.

AF2 is most likely the father of the child

7.A PCR rxn in w/c 4 different sets of primers are used to simultaneously amplify 4 distinct loci in the same rxn tube is known as a:

multiplex PCR reaction

8. Which of the ff is a known limitation of the uracil-N-glycosylase (UNG) chemistry commonly used for PCR contamination containment?

UNG will regain activity if temp drop below 55°C

9. A RNA sample is isolated from peripheral blood cells of a px. When performing spectrophotometric analysis to determine the yield of RNA in the sample u find 1:40dilution of 0.5mL sample gives OD 260= 0.03125 & an OD 280= 0.01760. What is the total amount of RNA contained in the 0.5mL sample?

25ug

10. The mode of inheritance of mitochondrial DNA:

maternal

11. What is the purpose of the enzyme uracil-N-glycolase in a molecular diagnostics laboratory?

to remove any previously generated amplicons containing dUTP from PCR rxn

12. The term that best describes males regarding X-linked genes is:

hemizygous

13. DNA-based testing for the presence of MRSA uses PCR with primers specific for which gene?

mecA

14. What is the most critical step in determining the specificity of a PCR reaction?

annealing temp

15. Methylation of cytosine residues is involved in the alteration of gene expression in a number of cancers. W/c sequencing technique is best suited to determine if methylation of cytosine residues has taken place in the promoter region of the given gene?

bisulfite sequencing

16. Which of the ff best depicts the general route of production in a human cell?

DNA-> mRNA -> protein

17. Which structure is most resistant to denaturation?

RNA:RNA duplexes

18. Which statement is true concerning the analysis of Short tandem repeats (STR)?

STR code for small cytokines

19.DENaturation of DNA during PCR rxn refers to breakinG:

Hydrogen bonds between nitrogenous bases in base-paired nucleotides

20. There is a bulge in the DNA double helix at a position 292 of a gene. This bulge is most likely due to which type of base mispairing?

purine:purine

21.W/c mtd of tissue preservation is inappropriate for molecular testing?

Mercuric chloride

22.common use 4 pulsed field gel electrophoresis is:

epidemiological typing of bacteria strains

23. Which nucleotide has a high frequency of mutation in human Chromosomal DNA?

cytosine

24. A translocation in w/c chromosome pair creates a BCR/ABL 1 fusion gene product asso with CML?

9;22

25. W/c rgt generates a signal during the annealing stage of a quantitative real time PCR reaction?

Molecular Beacon

26. The Kienow fragment of Escherichia coli DNA polymerase l is used:

in quantitative real time PCR

27. Monitoring the load of HIV circulating in a px plasma would best be accomplished using w/c method?

real-time nucleic acid amplification

28. W/c mtd of tissue preservation is inappropriate for molecular testing?

mercuric Cl

29. How many types of primers are used for PCR?

30. What is the conformation of DNA at 95°C?

single-stranded

31.What chemistry joins successive nucleoTIDES of DNA?

phosphodiester bond

32. A px sample contains 500particles/mL 200uL of sample is extracted,& DNA is eluted into 200uL. 5uL of DNA is added per PCR rxn .Calculate the ave no. of viral particles added per PCR rxn, assuming 100% extraction efficiency.

2.5

33. Which method for quantifying DNA would be most appropriate when testing small FFPE tissue sections by next generation sequencing?

agarose gel

34 DNA sequencing of hypervariable regions 1& 2 (HV1 & HV2) is used to:

detection of polymorphisms in human mitochondrial DNA

35. W/c sequence represents the replicate DNA strand 5'-GGG ATC GAT GCC CCT TAA AGA GTT TAC-3'

3'-CCC TAG CTA CGG GGA ATT TCT CAA ATG-5'

36. Probes are often used during real time PCR to quantitate the formation of specific amplicons during the reaction. Which system involves the use of 2 distinct probes to generate the fluorescent signal?

FRET

37. molecular-based diagnostic test for M. tuberculosis uses ribosomal RNA as the target. Printers are made to be specific for which RNA subunit.

16S

38. When quantifying the amount of genomic DNA in a sample by spectrophotometry. an OD 260 of 1.0 corresponds to what concentration of DNA?

50ug/mL

39. The 2 allele for a sex-linked recessive dse are X & x. The mutant allele is x. What is the percentage of male offspring that would be expected to be affected by the dse from parents who have the following genotypes?MOTHER= Xx FATHER= xY

40. Which locus is used to identify the gender of the individual from whom a DNA sample is obtained?

amelogenin

41. Identification of G(-) isolate using MALDI-TOF is based on detection of:

Ribosomal proteins

42. The real-time PCR curves represent dilutions of a single sample. Assuming PCR efficiency of 100%, what dilution is represented between aliquot A & aliquot B?

10-fold

43. Which condition has the highest stringency for DNA probe hybridization?

high temp, low salt conc

44. Which of the following nucleotides is a purINE?

adenine

45. What is the best method to rule out the possibility of genomic DNA giving a False(+) result in a reverse transcriptase (RT)PCR reaction?

use of upstream & downstream primers that span an exon-intron-exon region of the target

46. Reverse transcriptase would best be described as

RNA-dependent DNA polymerase

47. Intervening sequences are found in:

precursor mRNA

48. Amplification is most evident in which of PCR?

Log

49. In dideoxy chain termination sequencing (Sanger method), what does a heterozygous nucleotide position look like on an electropherogram?

2peaks in the same position, one twice the height of the other

50. Molecular-based testing was performed on a nasopharyngeal swap sample taken from a px. Results are reported as (+)lS481 (-)lS1000. These results indicate the px nasopharynx is colonized with:

Bordetella pertussis

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25問 • 3ヶ月前

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問題一覧

1.How many volumes of ethanol are added to1volume of DNA: salt soln to cause the DNA to precipitate?

2. What type of short read files with quality scores are used to storing next generation sequencing read data?

FASTQ

3.W/c is considered an epigenetic modification of DNA?

methylation of CpG islands in the promoter region of gene

4. What term best describes the relatedness of sequence, the percent identity or conservation?

similarity

5. What type of enzyme can be used to cut a chromosome into smaller fragments of DNA w/o removing any nucleotides or functional groups?

endonuclease

6. The results of STR typing of a child,the child's mom & 3 alleged dads(AF)of the child are listed in the table.

AF2 is most likely the father of the child

7.A PCR rxn in w/c 4 different sets of primers are used to simultaneously amplify 4 distinct loci in the same rxn tube is known as a:

multiplex PCR reaction

8. Which of the ff is a known limitation of the uracil-N-glycosylase (UNG) chemistry commonly used for PCR contamination containment?

UNG will regain activity if temp drop below 55°C

25ug

10. The mode of inheritance of mitochondrial DNA:

maternal

11. What is the purpose of the enzyme uracil-N-glycolase in a molecular diagnostics laboratory?

to remove any previously generated amplicons containing dUTP from PCR rxn

12. The term that best describes males regarding X-linked genes is:

hemizygous

13. DNA-based testing for the presence of MRSA uses PCR with primers specific for which gene?

mecA

14. What is the most critical step in determining the specificity of a PCR reaction?

annealing temp

bisulfite sequencing

16. Which of the ff best depicts the general route of production in a human cell?

DNA-> mRNA -> protein

17. Which structure is most resistant to denaturation?

RNA:RNA duplexes

18. Which statement is true concerning the analysis of Short tandem repeats (STR)?

STR code for small cytokines

19.DENaturation of DNA during PCR rxn refers to breakinG:

Hydrogen bonds between nitrogenous bases in base-paired nucleotides

20. There is a bulge in the DNA double helix at a position 292 of a gene. This bulge is most likely due to which type of base mispairing?

purine:purine

21.W/c mtd of tissue preservation is inappropriate for molecular testing?

Mercuric chloride

22.common use 4 pulsed field gel electrophoresis is:

epidemiological typing of bacteria strains

23. Which nucleotide has a high frequency of mutation in human Chromosomal DNA?

cytosine

24. A translocation in w/c chromosome pair creates a BCR/ABL 1 fusion gene product asso with CML?

9;22

25. W/c rgt generates a signal during the annealing stage of a quantitative real time PCR reaction?

Molecular Beacon

26. The Kienow fragment of Escherichia coli DNA polymerase l is used:

in quantitative real time PCR

27. Monitoring the load of HIV circulating in a px plasma would best be accomplished using w/c method?

real-time nucleic acid amplification

28. W/c mtd of tissue preservation is inappropriate for molecular testing?

mercuric Cl

29. How many types of primers are used for PCR?

30. What is the conformation of DNA at 95°C?

single-stranded

31.What chemistry joins successive nucleoTIDES of DNA?

phosphodiester bond

2.5

33. Which method for quantifying DNA would be most appropriate when testing small FFPE tissue sections by next generation sequencing?

agarose gel

34 DNA sequencing of hypervariable regions 1& 2 (HV1 & HV2) is used to:

detection of polymorphisms in human mitochondrial DNA

35. W/c sequence represents the replicate DNA strand 5'-GGG ATC GAT GCC CCT TAA AGA GTT TAC-3'

3'-CCC TAG CTA CGG GGA ATT TCT CAA ATG-5'

FRET

37. molecular-based diagnostic test for M. tuberculosis uses ribosomal RNA as the target. Printers are made to be specific for which RNA subunit.

16S

38. When quantifying the amount of genomic DNA in a sample by spectrophotometry. an OD 260 of 1.0 corresponds to what concentration of DNA?

50ug/mL

40. Which locus is used to identify the gender of the individual from whom a DNA sample is obtained?

amelogenin

41. Identification of G(-) isolate using MALDI-TOF is based on detection of:

Ribosomal proteins

42. The real-time PCR curves represent dilutions of a single sample. Assuming PCR efficiency of 100%, what dilution is represented between aliquot A & aliquot B?

10-fold

43. Which condition has the highest stringency for DNA probe hybridization?

high temp, low salt conc

44. Which of the following nucleotides is a purINE?

adenine

45. What is the best method to rule out the possibility of genomic DNA giving a False(+) result in a reverse transcriptase (RT)PCR reaction?

use of upstream & downstream primers that span an exon-intron-exon region of the target

46. Reverse transcriptase would best be described as

RNA-dependent DNA polymerase

47. Intervening sequences are found in:

precursor mRNA

48. Amplification is most evident in which of PCR?

Log

49. In dideoxy chain termination sequencing (Sanger method), what does a heterozygous nucleotide position look like on an electropherogram?

2peaks in the same position, one twice the height of the other

50. Molecular-based testing was performed on a nasopharyngeal swap sample taken from a px. Results are reported as (+)lS481 (-)lS1000. These results indicate the px nasopharynx is colonized with:

Bordetella pertussis