Analytical Techniques and Instrumentation 2

38問 • 1年前

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Refers to group of techniques used to separate complex mixtures on the basis of different physical interactions between the individual compounds and the stationary phase of the system.

Chromatography

the basic principle involve is the measurement of the intensity of the scattered light as a function of the concentration of the dispersed phase.

Nephelometry analysis

The measurement of intensity of the transmitted light is function of the concentration of the suspended particles,

Turbidimetric analysis

Involves the separation of soluble components in a solution by specific differences in physical- chemical characteristics of the different constituents

Chromatography

Fraction of the sugar and amino acid

Paper Chromatography

Used for drug screening (semiquantitative screening test)

Thin Layer Chromatography

relative distance of migration form the point of the application

Retention Factor

Extraction of drug and must be adjusted to reduce the solubility of the drug in the aqueous phase.

pH dependant

Used for the separation of steroids, barbiturates, blood, alcohol and lipids

Gas Chromatography

Gas Chromatography is useful for compounds that are naturally _____

Volatile

Flame ionization is used as detector

Gas Chromatography

Differences in absorption at the solid phase surfaces

Gas Solid Chromatography

Separation occurs by differences in solute partitioning between gaseous mobile phase and the liquid stationary phase

Gas Liquid Chromatography

Allows identification of substance by comparison with a computer library of a known fragmentation pattern.

Mass spectrometry

in Tandem Mass Spectroscopy how many inborn error metabolism detected?

Is based on the distribution of solutes between a liquid mobile phase and a stationary phase

Liquid Chromatography

Gold standard for drug testing and uses an electron beam to split the drug

GC-MS

Used for xenobiotics, anabolic steroids, pesticides

GC-MS

Uses pressure for fast separations, controlled temperature, in-line detectors and gradient elution technique

HPLC

Uses: Fraction of drugs, hormones lipids, carbohydrates, and proteins; separation and quantitation of various hemoglobins associated with specific diseases, rapid HbA1c

HPLC

mobile phase is more polar than the stationary phase.

Reverse Phase HPLC

Separates molecules based on differences in their size and shape.

Gel Permeation

For separation of enzymes, antibodies, and proteins.

Hydrophilic Gel (Gel Filtration)

supporting medium for Hydrophilic Gel

Dextran and Agarose

Supporting medium for Hydrophobic gel

Sephadex

For separation of triglycerides and fatty acids.

Hydrophobic Gel (Gel Permeation)

The exchange of the sample ions and mobile phase ions with charged group of the stationary phase

Ion Exchange Chromatography

For separation of amino acids, proteins and nucleic acids

Ion Exchange Chromatography

Liquid to liquid chromatography

Partition Chromatography

Separation compounds are based on their partition between liquid mobile phase and a liquid stationary phase coated on a solid support

Partition Chromatography

For separation of therapeutic drugs and their metabolites.

Partition Chromatography

What type of ligands are used as the stationary phase in affinity chromatography?

Biochemical ligands

What type of binding mechanism does affinity chromatography use?

Lock and key binding

Which of the following molecules can be separated using affinity chromatography?

All of the above

What is one of the main uses of affinity chromatography?

Preparation of large quantities of proteins and antibodies

What type of chromatography is absorption chromatography classified as?

Liquid-Solid Chromatography

What principle does absorption chromatography rely on for separation?

Differences between adsorption and desorption

What are common solid supports used in absorption chromatography?

Silica and alumina

PARA LEC PART 2

ユーザ名非公開 · 46問 · 1年前

PARA LEC PART 2