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BACTERIOLOGY ALL-PART 1

BACTERIOLOGY ALL-PART 1
80問 • 2年前
  • Chasny Halasan
    • 通報

    問題一覧

  • 1

    All cocci are gram positive except: Hint: No Boyfriend Vibe

    Neisseria, Branhamella, Veilonella

  • 2

    All bacilli are gram negative except: Hint: Big Lions Can Climb Every Large Mountain

    Bacillus, Listeria, Clostridium, Corynebacterium, Erysipelothrix, Lactobacillus, Mycobacterium

  • 3

    Developed the gram stain?

    Christian Gram

  • 4

    Gram stain of EXPECTORATED SPUTUM should reveal _______________ and ____________ per _______________.

    >10 EPITHELIAL CELLS, >25 WHITE BLOOD CELLS, LOW-POWER FIELD

  • 5

    Specimen should be transported IDEALLY WITHIN ___________ of collection

    30 minutes

  • 6

    2 specimen in which preservative can be use:

    Urine, Stool

  • 7

    This preservative maintain accurate urine colony counts at RT for 24 hours and useful for collection at distant locations

    boric acid

  • 8

    If stool specimen transportation delay longer than 2 hours, specimen can be added to this media?

    Cary-Blair Transport Media

  • 9

    Stools for C. difficile toxin assay should be collected WITHOUT PRESERVATIVE & can be REFRIGERATED; if the delay is longer than 48 hours, the specimen should be _________________

    Frozen at -70 C

  • 10

    most common anticoagulant used for microbiologic specimens; concentration must not exceed 0.025% wt/vol

    Sodium Polyanethol Sulfonate

  • 11

    SPS:Blood ratio

    1:5

  • 12

    This anticoagulant is often used for viral cultures and for isolation of Mycobacterium spp. from blood

    Heparin

  • 13

    Most commonly used transport media Don't put word Transport media

    Stuart's, Amie's

  • 14

    JEMBEC system stands for

    John E Martin Biological Environmental Chamber

  • 15

    This system contains selective agar and a CO₂-generating tablet. Specimen used is for N.gonorrhoeae

    JEMBEC

  • 16

    Any material containing the necessary nutritional and environmental requirements for bacterial growth

    Culture media

  • 17

    Best way to obtain pure culture is through

    Streak plate method

  • 18

    Steps in preparing plated culture media

    weigh, dissolve, sterilize, dispense

  • 19

    steps to prepare tubeddd

    Steps to prepare tubed culture media

  • 20

    without any trace of agar or solidifying substances (e.g. nutrient broth)

    LIQUID

  • 21

    contains 0.5-1.5% of agar (e.g. SIM)

    SEMISOLID

  • 22

    contains 2-3% of agar (e.g. BAP, TSI)

    SOLID

  • 23

    exact chemical composition of the ingredients are known (e.g. commercially prepared culture media)

    SYNTHETIC

  • 24

    precise composition of some or all of the nutritive substances used are known (e.g. meat extract broth)

    NON-SYNTHETIC

  • 25

    contains living tissue (e.g. embryonated egg for viruses)

    TISSUE CULTURE MEDIA

  • 26

    growth of most non-fastidious microbes (e.g. SHEEP BLOOD AGAR)

    NONSELECTIVE

  • 27

    growth of 1 type/group of microbes; it may contain certain inhibitory substances

    SELECTIVE

  • 28

    allow grouping of microbes based on different characteristics demonstrated on the medium

    DIFFERENTIAL

  • 29

    contain growth enhancers that are added to non-selective agar to allow fastidious organisms to flourish (e.g. CAP)

    ENRICHED

  • 30

    Shelf life of this culture media: tube with cotton-wool plug

    3 weeks

  • 31

    Shelf life of this culture media: tube with loose cap

    2 weeks

  • 32

    Shelf life of this culture media: container with screw cap

    3 months

  • 33

    Shelf life of this culture media: petri dishes

    4 weeks

  • 34

    Most bacteria cultures are incubated at what temperature?

    35 C to 37 C

  • 35

    Incubation time of bacterias?

    48-72 hours

  • 36

    Intact sheep red blood cells, vitamin K, yeast extract

    Anaerobic Blood Agar

  • 37

    Nutritious medium for the isolation and subculturing of obligate anaerobic bacteria

    Anaerobic Blood Agar

  • 38

    Hemolyzed sheep red blood cells supplemented with hemoglobin and NAD

    Chocolate Agar

  • 39

    Primary plating and subculturing of fastidious bacteria, e.g., Haemophilus and pathogenic Neisseria spp.

    Chocolate Agar

  • 40

    The carbohydrates lactose, salicin, and sucrose; and bile salts to inhibit growth of gram-positive bacteria and many nonpathogenic enteric bacteria

    Hektoen enteric agar

  • 41

    A selective and differential medium for the primary plating of stool specimens to aid in the recovery of intestinal pathogens, e.g., Salmonella

    Hektoen enteric agar

  • 42

    Lactose and bile salts, the low concentration of bile salts inhibits gram-positive bacteria but permits the growth of many gram-negative bacteria

    MacConkey agar

  • 43

    A selective and differential medium for the isolation of gram-negative bacteria; used for primary plating and subculturing

    MacConkey agar

  • 44

    Chocolate agar base containing the antimicrobial agents vancomycin, colistin, trimethoprim, and nystatin

    Modified Thayer-Martin agar

  • 45

    A selective primary plating medium for recovery of Neisseria gonorrhoeae and Neisseria meningitidis

    Modified Thayer-Martin agar

  • 46

    Dextrose (glucose), antimicrobials can be added to inhibit bacteria

    Sabouraud dextrose agar

  • 47

    Primary plating and subculturing of fungi

    Sabouraud dextrose agar

  • 48

    Intact sheep red blood cells

    Sheep Blood Agar

  • 49

    Primary plating and subculturing of most bacterial isolates

    Sheep Blood Agar

  • 50

    Media for susceptibility testing

    Mueller-Hinton Agar

  • 51

    Anaerobes and broth culture are held for how many days? IN RANGE!

    5-7 days

  • 52

    Selective enrichment media for S. agalactiae in female genital specimens Hint: double d and double t

    Todd-Hewitt Broth

  • 53

    This media is used for gram-positive organisms HINT: columbia is positive for mga hilason! CNA

    Columbia-Colistin-Nalidixic acid

  • 54

    Agar used for gram positive cocci and other anaerobic gram negative rods HINT: PEA

    Phenyl Ethyl Alcohol Agar

  • 55

    is partial lysing of RBCs in an SBA plate around and under the colony that results in a green discoloration of the medium.

    alpha hemolysis

  • 56

    is complete clearing of erythrocytes in SBA around or under the colonies because of the complete lysis of RBCs

    Beta hemolysis

  • 57

    Colonies of Bacillus anthracis on visual examination are described as “_____________” because of the filamentous appearance

    Medusa heads

  • 58

    This is defined as the hazy blanket of growth on the surface that extends well beyond the streak lines.

    swarming

  • 59

    S. pneumoniae typically produces _________ colonies, unless the colonies are mucoid because of the presence of a polysaccharide capsule.

    umbilicate

  • 60

    S. aureus typically produces __________ colonies.

    convex

  • 61

    In comparison, β-hemolytic streptococci generally produce _________ colonies.

    flat

  • 62

    β-Hemolytic streptococci except group B (S. agalactiae) are described as ?

    translucent

  • 63

    S. agalactiae produces colonies that are ?

    semiopaque

  • 64

    Staphylococci and other gram-positive bacteria are usually

    opaque

  • 65

    S. agalactiae with the organisms concentrated at the center of the colony, sometimes described as a

    Bull's-eye colony

  • 66

    S. aureus has what type of consistency

    creamy

  • 67

    Neisseria spp. has what type of consistency

    sticky

  • 68

    Nocardia spp. has what type of consistency

    brittle

  • 69

    Diphtheroid colonies has what type of consistency

    dry and waxy

  • 70

    Pigment of Pseudomonas aeruginosa?

    Metallic sheen

  • 71

    Pigment of serratia rubidaea

    Brick red

  • 72

    Pigment of Kluyvera spp.

    Blue

  • 73

    Pigment of Chromobacterium violaceum

    Purple

  • 74

    Pigment of Prevotella melaninogenica

    Brown-Black

  • 75

    Odor of S. aureus

    Old sock

  • 76

    Odor of P. aeruginosa

    fruity or grapelike

  • 77

    Odor of P. mirabilis

    putrid

  • 78

    Odor of Haemophilus spp.

    musty basement, mousy smell

  • 79

    Odor of Nocardia spp.

    freshly plowed field

  • 80

    No lysis

    Gamma Hemolysis

    • BACTE-PART 1

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    HCPT 1

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    HCPT 1

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    10問 • 2年前
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    COMPRE ON ENZYMES!!!!!!

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    TERMINOLOGIES

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    Chasny Halasan · 33問 · 2年前

    TERMINOLOGIES

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    33問 • 2年前
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    CREATINE KINASE

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    AMYLASE

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    AMYLASE

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    LIPASE

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    LIPASE

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    GAMMA-GLUTAMYL TRANSFERASE

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    Chasny Halasan · 10問 · 2年前

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    GLUCOSE-6-PHOSPHATE DEHYDROGENASE

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    ELECTROLYTES COMPRE

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    INTESTINAL NEMATODE

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    Chasny Halasan · 48問 · 1年前

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    TISSUE NEMATODES

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    FILARIAE AND CILIATE

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    COMPRE 1

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    RISK MANAGEMENT AND SAFETY

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    FIXATION

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    FIXATION

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    FIXATION: 1.ALDEHYDES

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    LAWS

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    LAWS

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    STAINING PROCEDURES

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    OTHER STAINS PART 1

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    OTHER STAIN PART 2

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    OTHER STAIN PART 2

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    THE HISTORY

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    THE HISTORY

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    THE ORIGIN

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    RA 5527

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    BASIC MYCOLOGY

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    Chasny Halasan · 23問 · 1年前

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    SYSTEMIC MYCOSES

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    SYSTEMIC MYCOSES

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    OPPORTUNISTIC MYCOSES

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    問題一覧

  • 1

    All cocci are gram positive except: Hint: No Boyfriend Vibe

    Neisseria, Branhamella, Veilonella

  • 2

    All bacilli are gram negative except: Hint: Big Lions Can Climb Every Large Mountain

    Bacillus, Listeria, Clostridium, Corynebacterium, Erysipelothrix, Lactobacillus, Mycobacterium

  • 3

    Developed the gram stain?

    Christian Gram

  • 4

    Gram stain of EXPECTORATED SPUTUM should reveal _______________ and ____________ per _______________.

    >10 EPITHELIAL CELLS, >25 WHITE BLOOD CELLS, LOW-POWER FIELD

  • 5

    Specimen should be transported IDEALLY WITHIN ___________ of collection

    30 minutes

  • 6

    2 specimen in which preservative can be use:

    Urine, Stool

  • 7

    This preservative maintain accurate urine colony counts at RT for 24 hours and useful for collection at distant locations

    boric acid

  • 8

    If stool specimen transportation delay longer than 2 hours, specimen can be added to this media?

    Cary-Blair Transport Media

  • 9

    Stools for C. difficile toxin assay should be collected WITHOUT PRESERVATIVE & can be REFRIGERATED; if the delay is longer than 48 hours, the specimen should be _________________

    Frozen at -70 C

  • 10

    most common anticoagulant used for microbiologic specimens; concentration must not exceed 0.025% wt/vol

    Sodium Polyanethol Sulfonate

  • 11

    SPS:Blood ratio

    1:5

  • 12

    This anticoagulant is often used for viral cultures and for isolation of Mycobacterium spp. from blood

    Heparin

  • 13

    Most commonly used transport media Don't put word Transport media

    Stuart's, Amie's

  • 14

    JEMBEC system stands for

    John E Martin Biological Environmental Chamber

  • 15

    This system contains selective agar and a CO₂-generating tablet. Specimen used is for N.gonorrhoeae

    JEMBEC

  • 16

    Any material containing the necessary nutritional and environmental requirements for bacterial growth

    Culture media

  • 17

    Best way to obtain pure culture is through

    Streak plate method

  • 18

    Steps in preparing plated culture media

    weigh, dissolve, sterilize, dispense

  • 19

    steps to prepare tubeddd

    Steps to prepare tubed culture media

  • 20

    without any trace of agar or solidifying substances (e.g. nutrient broth)

    LIQUID

  • 21

    contains 0.5-1.5% of agar (e.g. SIM)

    SEMISOLID

  • 22

    contains 2-3% of agar (e.g. BAP, TSI)

    SOLID

  • 23

    exact chemical composition of the ingredients are known (e.g. commercially prepared culture media)

    SYNTHETIC

  • 24

    precise composition of some or all of the nutritive substances used are known (e.g. meat extract broth)

    NON-SYNTHETIC

  • 25

    contains living tissue (e.g. embryonated egg for viruses)

    TISSUE CULTURE MEDIA

  • 26

    growth of most non-fastidious microbes (e.g. SHEEP BLOOD AGAR)

    NONSELECTIVE

  • 27

    growth of 1 type/group of microbes; it may contain certain inhibitory substances

    SELECTIVE

  • 28

    allow grouping of microbes based on different characteristics demonstrated on the medium

    DIFFERENTIAL

  • 29

    contain growth enhancers that are added to non-selective agar to allow fastidious organisms to flourish (e.g. CAP)

    ENRICHED

  • 30

    Shelf life of this culture media: tube with cotton-wool plug

    3 weeks

  • 31

    Shelf life of this culture media: tube with loose cap

    2 weeks

  • 32

    Shelf life of this culture media: container with screw cap

    3 months

  • 33

    Shelf life of this culture media: petri dishes

    4 weeks

  • 34

    Most bacteria cultures are incubated at what temperature?

    35 C to 37 C

  • 35

    Incubation time of bacterias?

    48-72 hours

  • 36

    Intact sheep red blood cells, vitamin K, yeast extract

    Anaerobic Blood Agar

  • 37

    Nutritious medium for the isolation and subculturing of obligate anaerobic bacteria

    Anaerobic Blood Agar

  • 38

    Hemolyzed sheep red blood cells supplemented with hemoglobin and NAD

    Chocolate Agar

  • 39

    Primary plating and subculturing of fastidious bacteria, e.g., Haemophilus and pathogenic Neisseria spp.

    Chocolate Agar

  • 40

    The carbohydrates lactose, salicin, and sucrose; and bile salts to inhibit growth of gram-positive bacteria and many nonpathogenic enteric bacteria

    Hektoen enteric agar

  • 41

    A selective and differential medium for the primary plating of stool specimens to aid in the recovery of intestinal pathogens, e.g., Salmonella

    Hektoen enteric agar

  • 42

    Lactose and bile salts, the low concentration of bile salts inhibits gram-positive bacteria but permits the growth of many gram-negative bacteria

    MacConkey agar

  • 43

    A selective and differential medium for the isolation of gram-negative bacteria; used for primary plating and subculturing

    MacConkey agar

  • 44

    Chocolate agar base containing the antimicrobial agents vancomycin, colistin, trimethoprim, and nystatin

    Modified Thayer-Martin agar

  • 45

    A selective primary plating medium for recovery of Neisseria gonorrhoeae and Neisseria meningitidis

    Modified Thayer-Martin agar

  • 46

    Dextrose (glucose), antimicrobials can be added to inhibit bacteria

    Sabouraud dextrose agar

  • 47

    Primary plating and subculturing of fungi

    Sabouraud dextrose agar

  • 48

    Intact sheep red blood cells

    Sheep Blood Agar

  • 49

    Primary plating and subculturing of most bacterial isolates

    Sheep Blood Agar

  • 50

    Media for susceptibility testing

    Mueller-Hinton Agar

  • 51

    Anaerobes and broth culture are held for how many days? IN RANGE!

    5-7 days

  • 52

    Selective enrichment media for S. agalactiae in female genital specimens Hint: double d and double t

    Todd-Hewitt Broth

  • 53

    This media is used for gram-positive organisms HINT: columbia is positive for mga hilason! CNA

    Columbia-Colistin-Nalidixic acid

  • 54

    Agar used for gram positive cocci and other anaerobic gram negative rods HINT: PEA

    Phenyl Ethyl Alcohol Agar

  • 55

    is partial lysing of RBCs in an SBA plate around and under the colony that results in a green discoloration of the medium.

    alpha hemolysis

  • 56

    is complete clearing of erythrocytes in SBA around or under the colonies because of the complete lysis of RBCs

    Beta hemolysis

  • 57

    Colonies of Bacillus anthracis on visual examination are described as “_____________” because of the filamentous appearance

    Medusa heads

  • 58

    This is defined as the hazy blanket of growth on the surface that extends well beyond the streak lines.

    swarming

  • 59

    S. pneumoniae typically produces _________ colonies, unless the colonies are mucoid because of the presence of a polysaccharide capsule.

    umbilicate

  • 60

    S. aureus typically produces __________ colonies.

    convex

  • 61

    In comparison, β-hemolytic streptococci generally produce _________ colonies.

    flat

  • 62

    β-Hemolytic streptococci except group B (S. agalactiae) are described as ?

    translucent

  • 63

    S. agalactiae produces colonies that are ?

    semiopaque

  • 64

    Staphylococci and other gram-positive bacteria are usually

    opaque

  • 65

    S. agalactiae with the organisms concentrated at the center of the colony, sometimes described as a

    Bull's-eye colony

  • 66

    S. aureus has what type of consistency

    creamy

  • 67

    Neisseria spp. has what type of consistency

    sticky

  • 68

    Nocardia spp. has what type of consistency

    brittle

  • 69

    Diphtheroid colonies has what type of consistency

    dry and waxy

  • 70

    Pigment of Pseudomonas aeruginosa?

    Metallic sheen

  • 71

    Pigment of serratia rubidaea

    Brick red

  • 72

    Pigment of Kluyvera spp.

    Blue

  • 73

    Pigment of Chromobacterium violaceum

    Purple

  • 74

    Pigment of Prevotella melaninogenica

    Brown-Black

  • 75

    Odor of S. aureus

    Old sock

  • 76

    Odor of P. aeruginosa

    fruity or grapelike

  • 77

    Odor of P. mirabilis

    putrid

  • 78

    Odor of Haemophilus spp.

    musty basement, mousy smell

  • 79

    Odor of Nocardia spp.

    freshly plowed field

  • 80

    No lysis

    Gamma Hemolysis