OTHER STAINS PART 1

OTHER STAINS PART 1
40問 • 1年前
  • Chasny Halasan
  • 通報

    問題一覧

  • 1

    Dyes that Are those obtained from plants and animals, previously utilized for dyeing of wool and cotton

    NATURAL DYE

  • 2

    The most important and the most commonly used for routine histologic studies

    Hematoxylin

  • 3

    A vegetable dye extracted from lichens which are normally colorless, but when treated with ammonia and expose to air, produce blue or violet colors

    ORCEIN

  • 4

    Mainly used for staining Elastic fibers

    ORCEIN

  • 5

    An old histologic dye extracted from the female Cochineal Bug (Coccus cacti)

    COCHINEAL

  • 6

    Cochineal is extracted from what organism?

    Coccus cacti

  • 7

    • Cochineal + Alum -used as a powerful chromatin and nuclear stain for fresh material and smear preparations

    Carmine

  • 8

    - • Carmine + Picric acid -used on neuropathological studies

    Picrocarmine

  • 9

    - • Carmine + Aluminum chloride -used for the demonstration of glycogen

    Best carmine

  • 10

    A plant with orange stigmas yielding a dye

    Saffron

  • 11

    a dye sometimes known as “coal tar dyes” since they were originally manufactured from substances that have been taken from Coal Tar.

    Synthetic dyes

  • 12

    - Substances with definite atomic groupings and are capable of producing visible colors.

    CHROMOPHORES

  • 13

    “color-bearers”

    CHROMOPHORES

  • 14

    “color increaser”

    AUXOCHROMES

  • 15

    - Auxiliary radicals or substances which impart to the compound the property of electrolytic dissociation, thereby altering the shade of the dye, giving it the property of forming salts with another compound and ultimately retaining its color.

    AUXOCHROMES

  • 16

    A process of selective removal of excess dye

    DIFFERENTIATION

  • 17

    - Act by combining with the metal thus breaking the latter’s union with the tissue or cell components - The acid chosen should be one that forms a soluble salt with the metal so that the latter is dissolved out - Ex. Hydrochloric acid and Acetic acid

    Acid differentiator

  • 18

    - Act by oxidizing the dye to a colorless substance (bleaching effect) - Components with the least dye will be bleached first - Ex. Potassium permanganate and Potassium ferricyanide

    Oxidizing differentiator

  • 19

    - When a section that have been stained by a mordant dye is placed in a solution of mordant, the latter is present in great excess and the dye gradually leaves the tissue to combine with the free mordant in solution

    Mordant differentiator

  • 20

    - A natural dye that is extracted from the core of heartwood of the tree, Haematoxylon campechianum. This tree was originally found in the state of Campeche, Mexico where the native population was familiar with its properties as a dye.

    HEMATOXYLIN

  • 21

    Hematoxylin dye is extracted from the core of heartwood of the tree called?

    Haematxylin campechianum

  • 22

    - By far the most valuable staining reagent used by the cytologist due to its powerful nuclear and active coloring agent:

    HEMATOXYLIN

  • 23

    formed by oxidation of Hematoxylin.

    HEMATIN

  • 24

    - Usually done by exposing the substance to air and sunlight, thereby oxidizing hematoxylin

    RIPENING

  • 25

    - The complex of stain and mordant is called a ________ ?

    LAKE

  • 26

    - Most commonly used mordant

    ALUMINUM

  • 27

    This mordant increase the selectivity for nuclei, especially if acid is added or is used as a differentiating agent

    ALUMINUM

  • 28

    causes an intense blue-black when used as a mordant

    FERRIC SALT

  • 29

    - Alum (potassium aluminum sulfate) in watery solution tends to dissociate the aluminum and it will combine with the OH (hydroxide) of the water to form insoluble aluminum hydroxide.

    BLUEING

  • 30

    An ALUM HEMATOXYLIN Oxidizing agent: Mercuric oxide

    HARRIS’ HEMATOXYLIN

  • 31

    - Oxidizing agent: Sodium iodate - The acid (glacial acetic acid) added to both gives stability to the stains and improves nuclear staining.

    MAYER'S HEMATOXYLIN

  • 32

    - Oxidizing agent: Alcoholic iodine - Recommended for routine purposes especially used in sequence with Celestine blue

    COLES HEMATOXYLIN

  • 33

    HEMATOXYLIN Used for the demonstration of spermatogenesis

    COPPER HEMATOXYLINS

  • 34

    - A red acid dye used for staining cytoplasm differentially

    EOSIN

  • 35

    - Commonly used as a background or contrast stain because it gives a pleasing and useful contrast to nuclear stains such as hematoxylin.

    EOSIN

  • 36

    Most commonly used EOSIN STAIN; YELLOWISH

    EOSIN Y

  • 37

    Bluish; deeper red color, rarely used EOSIN

    EOSIN B

  • 38

    eosin-alcohol soluble, rarely used EOSIN

    EOSIN S

  • 39

    Additional of this agent makes eosin staining more intense and selective

    glacial acetic

  • 40

    - A specialized fixative used in frozen section which serves to localize antigens and hydrolytic enzymes.

    Formol calcium

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    問題一覧

  • 1

    Dyes that Are those obtained from plants and animals, previously utilized for dyeing of wool and cotton

    NATURAL DYE

  • 2

    The most important and the most commonly used for routine histologic studies

    Hematoxylin

  • 3

    A vegetable dye extracted from lichens which are normally colorless, but when treated with ammonia and expose to air, produce blue or violet colors

    ORCEIN

  • 4

    Mainly used for staining Elastic fibers

    ORCEIN

  • 5

    An old histologic dye extracted from the female Cochineal Bug (Coccus cacti)

    COCHINEAL

  • 6

    Cochineal is extracted from what organism?

    Coccus cacti

  • 7

    • Cochineal + Alum -used as a powerful chromatin and nuclear stain for fresh material and smear preparations

    Carmine

  • 8

    - • Carmine + Picric acid -used on neuropathological studies

    Picrocarmine

  • 9

    - • Carmine + Aluminum chloride -used for the demonstration of glycogen

    Best carmine

  • 10

    A plant with orange stigmas yielding a dye

    Saffron

  • 11

    a dye sometimes known as “coal tar dyes” since they were originally manufactured from substances that have been taken from Coal Tar.

    Synthetic dyes

  • 12

    - Substances with definite atomic groupings and are capable of producing visible colors.

    CHROMOPHORES

  • 13

    “color-bearers”

    CHROMOPHORES

  • 14

    “color increaser”

    AUXOCHROMES

  • 15

    - Auxiliary radicals or substances which impart to the compound the property of electrolytic dissociation, thereby altering the shade of the dye, giving it the property of forming salts with another compound and ultimately retaining its color.

    AUXOCHROMES

  • 16

    A process of selective removal of excess dye

    DIFFERENTIATION

  • 17

    - Act by combining with the metal thus breaking the latter’s union with the tissue or cell components - The acid chosen should be one that forms a soluble salt with the metal so that the latter is dissolved out - Ex. Hydrochloric acid and Acetic acid

    Acid differentiator

  • 18

    - Act by oxidizing the dye to a colorless substance (bleaching effect) - Components with the least dye will be bleached first - Ex. Potassium permanganate and Potassium ferricyanide

    Oxidizing differentiator

  • 19

    - When a section that have been stained by a mordant dye is placed in a solution of mordant, the latter is present in great excess and the dye gradually leaves the tissue to combine with the free mordant in solution

    Mordant differentiator

  • 20

    - A natural dye that is extracted from the core of heartwood of the tree, Haematoxylon campechianum. This tree was originally found in the state of Campeche, Mexico where the native population was familiar with its properties as a dye.

    HEMATOXYLIN

  • 21

    Hematoxylin dye is extracted from the core of heartwood of the tree called?

    Haematxylin campechianum

  • 22

    - By far the most valuable staining reagent used by the cytologist due to its powerful nuclear and active coloring agent:

    HEMATOXYLIN

  • 23

    formed by oxidation of Hematoxylin.

    HEMATIN

  • 24

    - Usually done by exposing the substance to air and sunlight, thereby oxidizing hematoxylin

    RIPENING

  • 25

    - The complex of stain and mordant is called a ________ ?

    LAKE

  • 26

    - Most commonly used mordant

    ALUMINUM

  • 27

    This mordant increase the selectivity for nuclei, especially if acid is added or is used as a differentiating agent

    ALUMINUM

  • 28

    causes an intense blue-black when used as a mordant

    FERRIC SALT

  • 29

    - Alum (potassium aluminum sulfate) in watery solution tends to dissociate the aluminum and it will combine with the OH (hydroxide) of the water to form insoluble aluminum hydroxide.

    BLUEING

  • 30

    An ALUM HEMATOXYLIN Oxidizing agent: Mercuric oxide

    HARRIS’ HEMATOXYLIN

  • 31

    - Oxidizing agent: Sodium iodate - The acid (glacial acetic acid) added to both gives stability to the stains and improves nuclear staining.

    MAYER'S HEMATOXYLIN

  • 32

    - Oxidizing agent: Alcoholic iodine - Recommended for routine purposes especially used in sequence with Celestine blue

    COLES HEMATOXYLIN

  • 33

    HEMATOXYLIN Used for the demonstration of spermatogenesis

    COPPER HEMATOXYLINS

  • 34

    - A red acid dye used for staining cytoplasm differentially

    EOSIN

  • 35

    - Commonly used as a background or contrast stain because it gives a pleasing and useful contrast to nuclear stains such as hematoxylin.

    EOSIN

  • 36

    Most commonly used EOSIN STAIN; YELLOWISH

    EOSIN Y

  • 37

    Bluish; deeper red color, rarely used EOSIN

    EOSIN B

  • 38

    eosin-alcohol soluble, rarely used EOSIN

    EOSIN S

  • 39

    Additional of this agent makes eosin staining more intense and selective

    glacial acetic

  • 40

    - A specialized fixative used in frozen section which serves to localize antigens and hydrolytic enzymes.

    Formol calcium