- Differs from staining because it consists of an opaque black particulate precipitate

- The selective staining of living cell constituents, demonstrating cytoplasmic structures by phagocytosis of the dye particle (Cytoplasmic Phagocytosis).

Done by injecting the dye into any part of the animal body (either intravenous, intraperitoneal or subcutaneous) producing specific coloration of certain cells, particularly those of the reticuloendothelial system

- Has its greatest application in tissue from the CNS and for the demonstration of reticulin

The process whereby various constituents of tissues are studied thru chemical reactions that will permit microscopic localization of a specific tissue substance.

- Can behave as a stain and can outline the tissue elements in a non-particulate union

true vital staining of mitochondria

- Staining is continued in a definite sequence until the desired intensity of coloring of the different tissue elements is attained.

This staining is the basis of Histochemistry.

It is accomplished by controlled, specific chemical reactions designed to give a final color (staining) at the site/location of the structure of the substances in the cells or tissues.

The staining of tissue by means of simple alcoholic/aqueous solution of the dye

Metrachromatic staining for reticulocytes

- Makes use of heavy metals which are precipitated with selectivity of certain cellular and tissue components.

- Used to stain living cells immediately after removal from the living body

- Enables one to see and study the architectural pattern of the tissue and physical characteristics and structural relationships of tissues and their cells.

- Entails the use of the specific dyes that stains tissues with a color that is different from that of the stain color itself.

- Chemical substances that does not participate but merely increases or heightens the color intensity, selectivity, and crispness of the stain.

used for demonstration of lipids

most commonly used agent for impregnation

A classificaation of staining where it used to demonstrate the general relationship of tissues and cells with differentiation of nucleus and cytoplasm

- The tissues are overstained and the excess dye is then removed until the desired intensity is obtained.

- No washing/ differentiation/ decolorization in between is required solely relies on the selective affinity of dyes for different cellular elements.

- A substance which, when taken up by the tissue, helps make the dye in return serving as a link or bridge to make the staining reaction possible.

A combination of immunologic and histochemical techniques that allow phenotypic markers to be detected and demonstrated under the microscope, using a wide range of monoclonal, fluorescent-labeled or enzyme-labeled antibodies.

- The application of a different color or stain to provide contrast and background to the staining of the structural components to be demonstrated.

vital stain of RES (Reticuloendothelial System)

- The action of the dye is intensified by some other agents

A classificaation of staining in which the process whereby the tissue constituents are demonstrated in sections by direct interaction with a dye or staining solution, producing coloration of the active tissue component.