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Unit 4

Identification for Bacteria

Unit 4
33問 • 1年前
Identification for Bacteria
  • Bear4
    • 通報

    問題一覧

  • 1

    5 Methods of Identification Physical Characteristics Biochemical tests Serology Phage Typing Nucleic Acid analysis

    Bacterial

  • 2

    Cocci

    ball

  • 3

    Bacillus

    rod

  • 4

    Vibrio

    comma

  • 5

    -flesh eater -in ocean waters -infects/sometimes kills swimmers in FL

    Vibrio vulnificus

  • 6

    Spirillum

    lazy ‘S’

  • 7

    Spirochete

    tight corkscrew/coil

  • 8

    Filamentous

    ‘threads’

  • 9

    Spores ‘Stick pin’ is

    Clostridium tetani

  • 10

    Physical Characteristics

    Flagella

  • 11

    Can decide if bacterium is pathogenic or not

    Capsule

  • 12

    Defined = What the microbe eats/utilizes or, What the microbe produces (detected often by color changes)

    Biochemical Tests

  • 13

    What the bacterium utilizes, Use of sole carbon source ie., a sugar

    Biochemical Tests

  • 14

    Basis is Antibody-Antigen Complex Antibodies are very specific ie., Will only link to matching Antigen

    Serology

  • 15

    Antigen is pattern that is Non-self the body responds to as an attacker Body immune system attacks Source of Antigen (with cells, chemicals or Antibodies) Specific target Antibody to Lyme disease Will only link with Borrellia bacterium which causes Lyme

    Serology

  • 16

    Technique is called Agglutination

    How detect Serology

  • 17

    Serology/Agglutination

    Means antibody matches Antigen

  • 18

    grainy or clumping

    Serology/Agglutination (+)

  • 19

    smooth

    Serology/Agglutination (-)

  • 20

    Are produced in rabbits (also for cause of syphilis)

    Antibodies to Borrellia (Lyme disease)

  • 21

    A virus that will only kill One types of bacterium

    Phage Typing

  • 22

    Phage is A virus that only attacks/reproduces in Bacteria

    Bacteriophage

  • 23

    If virus and bacterial species Do Not match (Phage Typing)

    Then have solid growth of bacteria Over entire surface of plate

  • 24

    Virus kills bacteria where they ‘landed’ thus see clear areas of bacterial kill Called Plaques

    If virus and bacterial species Do match

  • 25

    Nucleic Acid Analysis - rRNA sequence (Woese)

    G to C base ratio

  • 26

    Has been used to discover /identify unculturable bacteria in environment

    rRNA sequence (Woese)

  • 27

    Make object stand out from background. (Often use Stains To obtain _____.)

    Contrast

  • 28

    Cannot see viruses With ______ Microscope!!

    Compound Light Microscope

  • 29

    Shifts light waves slightly out of or into ______To increase contrast See gray on gray better Good for living cells and internal organelles

    Phase Microscope

  • 30

    Light enters as ultraviolet (you cannot see uv) Pigment or compound/dye absorbs uv Pigment or compound/dye shifts wavelength Light re-emitted in visible range (Now you can see it) Clostridium

    Fluorescent Microscope

  • 31

    Immunofluorescent technique Link fluorescent dye to end of Antibody Antibody links to specific target (Example, strain of bacteria in sample) Antibody covers target but has ‘flashlight’ on end Target ‘lights up’ = is fluorescent Rest of sample remains dark

    Fluorescent Microscope

  • 32

    Immunofluorescent technique Lyme Disease spirochete

    Fluorescent Microscope

  • 33

    Uses Magnets to direct Electron Beam To focus

    Electron Microscope

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    問題一覧

  • 1

    5 Methods of Identification Physical Characteristics Biochemical tests Serology Phage Typing Nucleic Acid analysis

    Bacterial

  • 2

    Cocci

    ball

  • 3

    Bacillus

    rod

  • 4

    Vibrio

    comma

  • 5

    -flesh eater -in ocean waters -infects/sometimes kills swimmers in FL

    Vibrio vulnificus

  • 6

    Spirillum

    lazy ‘S’

  • 7

    Spirochete

    tight corkscrew/coil

  • 8

    Filamentous

    ‘threads’

  • 9

    Spores ‘Stick pin’ is

    Clostridium tetani

  • 10

    Physical Characteristics

    Flagella

  • 11

    Can decide if bacterium is pathogenic or not

    Capsule

  • 12

    Defined = What the microbe eats/utilizes or, What the microbe produces (detected often by color changes)

    Biochemical Tests

  • 13

    What the bacterium utilizes, Use of sole carbon source ie., a sugar

    Biochemical Tests

  • 14

    Basis is Antibody-Antigen Complex Antibodies are very specific ie., Will only link to matching Antigen

    Serology

  • 15

    Antigen is pattern that is Non-self the body responds to as an attacker Body immune system attacks Source of Antigen (with cells, chemicals or Antibodies) Specific target Antibody to Lyme disease Will only link with Borrellia bacterium which causes Lyme

    Serology

  • 16

    Technique is called Agglutination

    How detect Serology

  • 17

    Serology/Agglutination

    Means antibody matches Antigen

  • 18

    grainy or clumping

    Serology/Agglutination (+)

  • 19

    smooth

    Serology/Agglutination (-)

  • 20

    Are produced in rabbits (also for cause of syphilis)

    Antibodies to Borrellia (Lyme disease)

  • 21

    A virus that will only kill One types of bacterium

    Phage Typing

  • 22

    Phage is A virus that only attacks/reproduces in Bacteria

    Bacteriophage

  • 23

    If virus and bacterial species Do Not match (Phage Typing)

    Then have solid growth of bacteria Over entire surface of plate

  • 24

    Virus kills bacteria where they ‘landed’ thus see clear areas of bacterial kill Called Plaques

    If virus and bacterial species Do match

  • 25

    Nucleic Acid Analysis - rRNA sequence (Woese)

    G to C base ratio

  • 26

    Has been used to discover /identify unculturable bacteria in environment

    rRNA sequence (Woese)

  • 27

    Make object stand out from background. (Often use Stains To obtain _____.)

    Contrast

  • 28

    Cannot see viruses With ______ Microscope!!

    Compound Light Microscope

  • 29

    Shifts light waves slightly out of or into ______To increase contrast See gray on gray better Good for living cells and internal organelles

    Phase Microscope

  • 30

    Light enters as ultraviolet (you cannot see uv) Pigment or compound/dye absorbs uv Pigment or compound/dye shifts wavelength Light re-emitted in visible range (Now you can see it) Clostridium

    Fluorescent Microscope

  • 31

    Immunofluorescent technique Link fluorescent dye to end of Antibody Antibody links to specific target (Example, strain of bacteria in sample) Antibody covers target but has ‘flashlight’ on end Target ‘lights up’ = is fluorescent Rest of sample remains dark

    Fluorescent Microscope

  • 32

    Immunofluorescent technique Lyme Disease spirochete

    Fluorescent Microscope

  • 33

    Uses Magnets to direct Electron Beam To focus

    Electron Microscope