ENZYME OF CLINICAL SIGNIFICANCE-MCQ

99問 • 1ヶ月前

ayrika

通報

問題一覧

EC 2.6.1.1

AST

SGOT

AST

Serves as an important biomarker for liver, muscle disorders, and cardiac events

AST

Marker for the detection and monitoring of liver disease

ALT

EC 2.6.1.2

ALT

EC 2.7.3.2

EC 1.1.1.27

LDH

An important oxidoreductase enzyme that catalyzes the interconversion of lactate and pyruvate

LDH

EC 3.1.3.2

ACP

A hydrolase enzyme tha catalyzes the hydrolysis of phosphate esters in acidic conditions

ACP

EC 3.1.3.1

ALP

Catalyzes the hydrolysis of phosphate groups from various molecules such as nucleotides proteins and alkaloids under alkaline conditions

ALP

EC 2.3.2.1

GGT

Catalyzes the transfer of y glutamyl group from y glutamyl peptides like glutathione

GGT

Highest sensitivity for the detection of liver disorders

GGT

Full name is A1, 1-4 glucan-4-glucanohydrolase

AMS

EC 3.2.1.1

AMS

Smallest enzyme which could easily pass through glomeruli

AMS

Catalyzes the hydrolysis of triglycerides esters of long chain fatty acid

LPS

EC 3.1.1.3

LPS

Chemical name is Triaglycerol Acylhydrolase

LPS

EC 3.4.21.4

TRYP

AST-AMI Pre Peak Return

6-8 HRS, 24 HRS, 3-5 DAY

CK-AMI Pre Peak Return

4-8 HRS, 12-24 HRS, 2-3 DAY

LDH-AMI Pre Peak Return

12-24 HRS, 2-3 DAY, 10-14 DAY

AMS-Pancreatitis Pre Peak Return

7-12 HRS, 12-72 HRS, 3-5 DAY

LPS-Pancreatitis Pre Peak Return

4-8 HRS, 24 HRS, 7-14 DAY

AST ULN Viral hep

100X

AST ULN Cirrhosis

AST ULN Skeletal muscle disorder

4-8X

De ritis ratio formula

AST/ALT

De Ritis Ratio <1

NORMAL, VIRAL HEP

De Ritis Ratio 1-2

CHRONIC LIVER, CIRRHOSIS

De Ritis Ratio >2

ALCOHOLIC HEP, HEP/CEL CARCINOMA

AST Stability

3-4 DAY AT 4-8C

ALT Stability

3-4 DAY AT 4 C

Assay for AST

KARMEN METHOD

Assay for ALT

LDH COUPLED METHOD

Substrate for AST

MDH

Substrate for ALT

LDH

Optimal pH for Karmen

7.3-7.8

Hemolysis in AST may increase result

15X

Obligate activator for CK

MAGNESIUM

Molecular structure of CK

DIMERIC, 40,000 D

CK complexed w/ Ig

MACRO CK TYPE 1

CK composed of oligomeric CK MT

MACRO CK TYPE 2

Macro CK found in Severe illness, malignancy, and liver disease

TYPE 2

Macro CK found in ADA, Old indivs,and Chronic illness

TYPE 1

Macro CK attach to IgG

CK BB

Macro CK attach to IgA

CK MM

Helpful in detecting reoccurence of MI

CK MB

Which CK can be used as tumor marker potential;

CK BB

Optimum pH for CK forward reaction

9.0

Optimum pH for CK reverse reaction

6.7

Forward reaction step for CK

CK REACTION, PEP SYSTEM, LDH SYSTEM

Reverse reaction step for CK

PRIMARY CK REACTION, HK SYSTEM, G6PD SYSTEM

Which reaction requires magnesium in CK

FORWARD

Reference method for CK based on net charge and mobility

ELECTROPHORESIS

High resolution separation based on affinity to charged resin

ION EXCHANGE

CK determination which uses anti m or anti b

IMMUNOINHIBITION

CK determination which uses mono or polyclonal ab detecting active and inactive form

IMMUNOASSAY

CK band source of misID in hemodialysis pxt during electrophoresis

CK 1

Can quantify CK MB mass concentration

IMMUNOASSAY

May merge to CK MB

CK MM

May co elute to CK MB

CK BB

Can also co elute with CK mb

MACRO CK

Optimum pH for oliver rosalki method

6.8

LDH band used for detection of Pancreatitis

LDu c'monly used measures NANDH formed less product inhibition

WACKER

2x faster used in dry slide tech requiring less specimen

WROBLEUSKI LA DUE

LDH forward reaction

8.8-9.8

LDH reverse reaction

7.4-7.8

Wrobleuski la due pH

7.2

Increases in mild hepatic injury

cAST

Increases in severe hepatic injury

mAST

Serves as rapid energy reserve capable of regenerating ATP from ADP during muscle contraction

PHOSPHOCREATINE

May constitute 15% of total CK activity of the heart

CK-Mt

Condition may elevate CK for up to 50X ULN

DMD

Caused by the reduced cerebral blood flow due to throbotic or amblic stroke

CEREBRAL ISCHEMIA

This leads to neuronal necrosis and CK BB leakage into CSF and occasionally serum

CELLULAR HYPOXIA

Encompasses ischemic stroke intracerebral hemorrhage and subarachnoid hemorrhage

ACUTE CEREBRAVASCULAR DISEASE

Acute metabolic encelopathy in chlidren following viral illness and aspirin use

REYES SYNDROME

What ck elevates in Reyes syndrome

CK BB, CK MM

This step is spectrochemically monitored by measruing the decrease in NADH absorbance at 340 nm

FORWARD, LDH SYSTEM

This prevents equilibrium build up and maintains the forward flow of the CK reaction

REVERSE, HK SYSTEM

NADPH is formed and measured spectrochemically at 340 nm

REVERSE, G6PD SYSTEM

Enzyme ubiquitous in nature

LDH

Molecular structure of LDH

TETRAMER

Signifies grave prognosis and impending death

LDH 6

Substrate to enhance LDH 1

A HYDROXYBUTYRATE

Hemolysis may increase result of LDH for

100-150X

Heat labile LDH

1, 2

Heat stable LDH

4, 5

Most unstable LDH

Optimum pH of ACP

5.0

Which ACP is prostatic tissue and tartrate sensitive

Which ACP is granulocyte minor contributor

2, 4

Which ACP is Plateles Rbcs, and monocytes; a major isoenzyme in plasma

TRAP Bone ACP

DEMATIACEOUS OPPORTUNISTIC FUNGI

ayrika · 52問 · 2ヶ月前

DEMATIACEOUS OPPORTUNISTIC FUNGI

52問 • 2ヶ月前

ayrika

PITUITARY GLAND PART 2

ayrika · 5問 · 1ヶ月前

PITUITARY GLAND PART 2

5問 • 1ヶ月前

ayrika

PITUITARY GLAND PART 2

ayrika · 5問 · 1ヶ月前

PITUITARY GLAND PART 2

THYROID GLAND

THYROID GLAND

THYROID GLAND

THYROID GLAND

ADRENAL GLAND

ADRENAL GLAND

SEX GLANDS

SEX GLANDS

OTHER GLANDS

OTHER GLANDS

OTHER GLANDS

OTHER GLANDS

TOXICOLOGY

TOXICOLOGY

TOXICOLOGY

TOXICOLOGY

TOXICOLOGY

TOXICOLOGY

DRUG OF ABUSE

DRUG OF ABUSE

DRUG OF ABUSE

DRUG OF ABUSE

TOXINS

TOXINS

TOXINS

TOXINS

TDM

TDM

TDM

TDM

問題一覧

EC 2.6.1.1

AST

SGOT

AST

Serves as an important biomarker for liver, muscle disorders, and cardiac events

AST

Marker for the detection and monitoring of liver disease

ALT

EC 2.6.1.2

ALT

EC 2.7.3.2

EC 1.1.1.27

LDH

An important oxidoreductase enzyme that catalyzes the interconversion of lactate and pyruvate

LDH

EC 3.1.3.2

ACP

A hydrolase enzyme tha catalyzes the hydrolysis of phosphate esters in acidic conditions

ACP

EC 3.1.3.1

ALP

Catalyzes the hydrolysis of phosphate groups from various molecules such as nucleotides proteins and alkaloids under alkaline conditions

ALP

EC 2.3.2.1

GGT

Catalyzes the transfer of y glutamyl group from y glutamyl peptides like glutathione

GGT

Highest sensitivity for the detection of liver disorders

GGT

Full name is A1, 1-4 glucan-4-glucanohydrolase

AMS

EC 3.2.1.1

AMS

Smallest enzyme which could easily pass through glomeruli

AMS

Catalyzes the hydrolysis of triglycerides esters of long chain fatty acid

LPS

EC 3.1.1.3

LPS

Chemical name is Triaglycerol Acylhydrolase

LPS

EC 3.4.21.4

TRYP

AST-AMI Pre Peak Return

6-8 HRS, 24 HRS, 3-5 DAY

CK-AMI Pre Peak Return

4-8 HRS, 12-24 HRS, 2-3 DAY

LDH-AMI Pre Peak Return

12-24 HRS, 2-3 DAY, 10-14 DAY

AMS-Pancreatitis Pre Peak Return

7-12 HRS, 12-72 HRS, 3-5 DAY

LPS-Pancreatitis Pre Peak Return

4-8 HRS, 24 HRS, 7-14 DAY

AST ULN Viral hep

100X

AST ULN Cirrhosis

AST ULN Skeletal muscle disorder

4-8X

De ritis ratio formula

AST/ALT

De Ritis Ratio <1

NORMAL, VIRAL HEP

De Ritis Ratio 1-2

CHRONIC LIVER, CIRRHOSIS

De Ritis Ratio >2

ALCOHOLIC HEP, HEP/CEL CARCINOMA

AST Stability

3-4 DAY AT 4-8C

ALT Stability

3-4 DAY AT 4 C

Assay for AST

KARMEN METHOD

Assay for ALT

LDH COUPLED METHOD

Substrate for AST

MDH

Substrate for ALT

LDH

Optimal pH for Karmen

7.3-7.8

Hemolysis in AST may increase result

15X

Obligate activator for CK

MAGNESIUM

Molecular structure of CK

DIMERIC, 40,000 D

CK complexed w/ Ig

MACRO CK TYPE 1

CK composed of oligomeric CK MT

MACRO CK TYPE 2

Macro CK found in Severe illness, malignancy, and liver disease

TYPE 2

Macro CK found in ADA, Old indivs,and Chronic illness

TYPE 1

Macro CK attach to IgG

CK BB

Macro CK attach to IgA

CK MM

Helpful in detecting reoccurence of MI

CK MB

Which CK can be used as tumor marker potential;

CK BB

Optimum pH for CK forward reaction

9.0

Optimum pH for CK reverse reaction

6.7

Forward reaction step for CK

CK REACTION, PEP SYSTEM, LDH SYSTEM

Reverse reaction step for CK

PRIMARY CK REACTION, HK SYSTEM, G6PD SYSTEM

Which reaction requires magnesium in CK

FORWARD

Reference method for CK based on net charge and mobility

ELECTROPHORESIS

High resolution separation based on affinity to charged resin

ION EXCHANGE

CK determination which uses anti m or anti b

IMMUNOINHIBITION

CK determination which uses mono or polyclonal ab detecting active and inactive form

IMMUNOASSAY

CK band source of misID in hemodialysis pxt during electrophoresis

CK 1

Can quantify CK MB mass concentration

IMMUNOASSAY

May merge to CK MB

CK MM

May co elute to CK MB

CK BB

Can also co elute with CK mb

MACRO CK

Optimum pH for oliver rosalki method

6.8

LDH band used for detection of Pancreatitis

LDu c'monly used measures NANDH formed less product inhibition

WACKER

2x faster used in dry slide tech requiring less specimen

WROBLEUSKI LA DUE

LDH forward reaction

8.8-9.8

LDH reverse reaction

7.4-7.8

Wrobleuski la due pH

7.2

Increases in mild hepatic injury

cAST

Increases in severe hepatic injury

mAST

Serves as rapid energy reserve capable of regenerating ATP from ADP during muscle contraction

PHOSPHOCREATINE

May constitute 15% of total CK activity of the heart

CK-Mt

Condition may elevate CK for up to 50X ULN

DMD

Caused by the reduced cerebral blood flow due to throbotic or amblic stroke

CEREBRAL ISCHEMIA

This leads to neuronal necrosis and CK BB leakage into CSF and occasionally serum

CELLULAR HYPOXIA

Encompasses ischemic stroke intracerebral hemorrhage and subarachnoid hemorrhage

ACUTE CEREBRAVASCULAR DISEASE

Acute metabolic encelopathy in chlidren following viral illness and aspirin use

REYES SYNDROME

What ck elevates in Reyes syndrome

CK BB, CK MM

This step is spectrochemically monitored by measruing the decrease in NADH absorbance at 340 nm

FORWARD, LDH SYSTEM

This prevents equilibrium build up and maintains the forward flow of the CK reaction

REVERSE, HK SYSTEM

NADPH is formed and measured spectrochemically at 340 nm

REVERSE, G6PD SYSTEM

Enzyme ubiquitous in nature

LDH

Molecular structure of LDH

TETRAMER

Signifies grave prognosis and impending death

LDH 6

Substrate to enhance LDH 1

A HYDROXYBUTYRATE

Hemolysis may increase result of LDH for

100-150X

Heat labile LDH

1, 2

Heat stable LDH

4, 5

Most unstable LDH

Optimum pH of ACP

5.0

Which ACP is prostatic tissue and tartrate sensitive

Which ACP is granulocyte minor contributor

2, 4

Which ACP is Plateles Rbcs, and monocytes; a major isoenzyme in plasma

TRAP Bone ACP