IMPREGNATION AND EMBEDDING

21問 • 2年前

Kmyching

通報

問題一覧

Simplest, most common and best embedding medium .Very rapid

PARAFFIN WAX IMPREGNATION

Melting point

56°C

If the lab temp is between 20-24

54- 58C

If the lab temp is between 15-18

50- 54C

Wax with melting point = 56 C is normally used for routine work

MANUAL PROCESSING

This method makes use of an automatic tissue processing machine (Autotechnicon).Usually only 2-3 changes of wax are required to remove the clearing agent

AUTOMATIC PROCESSING

involves the wax impregnation under negative atmospheric pressure inside an embedding oven. Provides more rapid wax penetration of tissue

VACUUM EMBEDDING

mixture of highly purified paraffin and synthetic plastic polymers. for large dense tissue blocks such as bones and brain

Paraplast= MP: 56-57 C

less brittle and less compressible than paraplast.

Embeddol MP: 56-58 C

synthetic wax

Bioloid

product of paraffin, containing rubber, with the same property as paraplast.

Tissue mat

harder than paraffin.not soluble in water.soluble in 95% ETOH and other clearing agents.

Ester wax

soluble and miscible with water (hence does not require dehydration and clearing of the tissue).

CARBOWAX

AKA colloidin Very slow (days to weeks) Methods

CELLOIDIN

Rarely used Water soluble Add 1% phenol to prevent molds

GELATIN

Done after wax impregnation Placing the tissue in a mold containing the medium

EMBEDDING/CASTING/BLOCKING

process by which thee tissue is arranged in a precise positions in the mold

Orientation

Plastic (resin)embedding classified into

Double embedding method

reduces antigenicity, toxic and damage tissue

EPOXY

not often used

POLYESTER

used extensively for light microscopy

ACRYLIC

HISTOPATH (lesson 1)

Kmyching · 56問 · 2年前

HISTOPATH (lesson 1)

56問 • 2年前

Kmyching

bacte (microbial control)

Kmyching · 26問 · 2年前

bacte (microbial control)

Collection

Collection

Histopath (somatic death)

Kmyching · 25問 · 2年前

Histopath (somatic death)

fresh tissue

fresh tissue

Staphylococcus

Staphylococcus

lesson 1 (cell injury, adaptation and inflammation and repair)

Kmyching · 67問 · 2年前

lesson 1 (cell injury, adaptation and inflammation and repair)

biopsy

biopsy

autopsy

autopsy

decalcification

decalcification

DEHYDRATION AND CLEARING

Kmyching · 27問 · 2年前

DEHYDRATION AND CLEARING

27問 • 2年前

Kmyching

TRIMMING AND SECTIONING

Kmyching · 35問 · 2年前

TRIMMING AND SECTIONING

35問 • 2年前

Kmyching

adhesive and staining

Kmyching · 43問 · 2年前

adhesive and staining

43問 • 2年前

Kmyching

COMMON STAINING SOLUTION

Kmyching · 19問 · 1年前

COMMON STAINING SOLUTION

OTHER STAINING

OTHER STAINING

H&E STAINING, MOUNTING, RINGING AND LABELLING

Kmyching · 19問 · 1年前

H&E STAINING, MOUNTING, RINGING AND LABELLING

IMMUNOHISTOCHEMISTRY

IMMUNOHISTOCHEMISTRY

EPITHELIAL TUMOR MARKERS

Kmyching · 5問 · 1年前

EPITHELIAL TUMOR MARKERS

5問 • 1年前

Kmyching

INTERMEDIATE FILAMENT MARKERS

Kmyching · 6問 · 1年前

INTERMEDIATE FILAMENT MARKERS

6問 • 1年前

Kmyching

NEUROENDOCRINE MARKERS and GERM CELL TUMOR MARKERS

Kmyching · 6問 · 1年前

NEUROENDOCRINE MARKERS and GERM CELL TUMOR MARKERS

6問 • 1年前

Kmyching

MESENCHYMAL TUMOR MARKERS

Kmyching · 5問 · 1年前

MESENCHYMAL TUMOR MARKERS

5問 • 1年前

Kmyching

問題一覧

Simplest, most common and best embedding medium .Very rapid

PARAFFIN WAX IMPREGNATION

Melting point

56°C

If the lab temp is between 20-24

54- 58C

If the lab temp is between 15-18

50- 54C

Wax with melting point = 56 C is normally used for routine work

MANUAL PROCESSING

This method makes use of an automatic tissue processing machine (Autotechnicon).Usually only 2-3 changes of wax are required to remove the clearing agent

AUTOMATIC PROCESSING

involves the wax impregnation under negative atmospheric pressure inside an embedding oven. Provides more rapid wax penetration of tissue

VACUUM EMBEDDING

mixture of highly purified paraffin and synthetic plastic polymers. for large dense tissue blocks such as bones and brain

Paraplast= MP: 56-57 C

less brittle and less compressible than paraplast.

Embeddol MP: 56-58 C

synthetic wax

Bioloid

product of paraffin, containing rubber, with the same property as paraplast.

Tissue mat

harder than paraffin.not soluble in water.soluble in 95% ETOH and other clearing agents.

Ester wax

soluble and miscible with water (hence does not require dehydration and clearing of the tissue).

CARBOWAX

AKA colloidin Very slow (days to weeks) Methods

CELLOIDIN

Rarely used Water soluble Add 1% phenol to prevent molds

GELATIN

Done after wax impregnation Placing the tissue in a mold containing the medium

EMBEDDING/CASTING/BLOCKING

process by which thee tissue is arranged in a precise positions in the mold

Orientation

Plastic (resin)embedding classified into

Double embedding method

reduces antigenicity, toxic and damage tissue

EPOXY

not often used

POLYESTER

used extensively for light microscopy

ACRYLIC