問題一覧
1
Type or Condensation
Orthotoluidine
2
Principle: Coupling reaction using G6PD (highly specific) and is measured by quantitating reduced NADPH formation at 340 nm
Hexokinase Method
3
Assess LONG TERM glucose control (2-3 months)
HbA1C
4
Excellent for glucose determination
Hexokinase Method
5
The patient shouls vomit after drinking glucose load
False
6
Capillary blood glucose is __________ compared to venous blood glucose
2 to 5 mg/dL higher
7
What type of tube does venous plasma glucose uses?
Gray tube
8
Principle: Measures oxygen consumption using an electrode. Oxygen depletion is DIRECTLY PROPORTIONAL to the amount of glucose present.
Polarographic Method
9
Principle: Inverse colorimetry
Hagedorn Jensen
10
Used for diagnosis except in patients with hemoglobinopathies or abnormal RBC turnover
HbA1C
11
Assess glucose control over a period of 2 to 3 weeks
Fructosamine
12
Principle: Glucose is reduced to produce a chromophore that is measured spectrophotometrically or an electrical current. The amount of NADPH generated is PROPORTIONAL to the glucose determination.
Glucose Dehydrogenase
13
Principle: Protein precipitation
Ortho-toluidine
14
how many grams of glucose load needed for an adult?
75g
15
H2O2 is removed by two additional reactions called:
Catalase , Molybdate
16
Is veins deoxygenated or oxygenated?
Deoxygenated
17
Values for fasting plasma glucose
70 to 110 mg/dL
18
Finish drinking glucose within ______
5 minutes
19
End color of ortho-toluidine at 630 nm
Green
20
Is arteries deoxygenated or oxygenated?
Oxygenated
21
Reference method / Most specific glucose method
Hexokinase Method
22
Standard Specimen for specimen collecion
Venous Plasma Glucose
23
Principle: Cupric ions reduced to cuprous ions forming cuprous oxide in hot alkaline solution by glucose
Cupric Ion Reduction
24
Critical / panic values
<40 mg/dL, >500 mg/dL
25
Reagent used for Hagedorn Jensen/Ferric reduction
Hot alkaline solution of potassium ferricyanide
26
It covers alpha glucose to beta glucose
Mutarotase
27
Interfering substance used in ortho-toluidine
Galactose and Mannose
28
Allowable delay (separation of cells from serum)
30 to 60 minutes
29
Types of glucose oxidase
Colorimetric , Polarographic
30
Standare method for HbA1C
HPLC-gold standard method
31
Chromogens used colorimetric-saifer gernstenfield
3-methyl-2-benzothiazolinone hydrazine, N-N-dimethylaniline, O-dianisidine, Phenol
32
Methods used for IVGTT
Janney-Isaacson
33
Types of Oxidation Reduction
Folin Wu, Nelson Somogyi, Neocuproine, Benedict’s Method
34
Is capillaries deoxygenated or oxygenated?
Both deoxygenated and oxygenated
35
What type of anticoagulant does venous plasma specimen uses?
Sodium Fluoride
36
Fasting glucose in whole blood is _____________ compared to serum/plasma glucose
10 to 15% lower
37
Renal threshold for glucose:
160 to 180 mg/dL
38
Types of Enzymatic
Glucose Oxidase, Hexokinase, Glucose Dehydrogenase
39
Principle: Uses TRINDER REACTION
Colorimetric-Saifer Gernstenfield
40
How many fasting hours does Oral Glucose Tolerance Test (OGTT) needed?
at least 8 hours
41
Give the intefering substances of colorimteric-saifer gernstenfield
Ascorbic Acid , Glutathione , Uric Acid, Bilirubin, Hemoglobin, Creatinine, Dopamine , Methyldopa, Citric Acid
42
Fasting hours required?
8-10 hours