問題一覧
1
Standard Specimen for specimen collecion
Venous Plasma Glucose
2
Fasting glucose in whole blood is _____________ compared to serum/plasma glucose
10 to 15% lower
3
Capillary blood glucose is __________ compared to venous blood glucose
2 to 5 mg/dL higher
4
Is capillaries deoxygenated or oxygenated?
Both deoxygenated and oxygenated
5
Is veins deoxygenated or oxygenated?
Deoxygenated
6
Is arteries deoxygenated or oxygenated?
Oxygenated
7
What type of tube does venous plasma glucose uses?
Gray tube
8
What type of anticoagulant does venous plasma specimen uses?
Sodium Fluoride
9
Fasting hours required?
8-10 hours
10
Allowable delay (separation of cells from serum)
30 to 60 minutes
11
Renal threshold for glucose:
160 to 180 mg/dL
12
Assess LONG TERM glucose control (2-3 months)
HbA1C
13
Assess glucose control over a period of 2 to 3 weeks
Fructosamine
14
How many fasting hours does Oral Glucose Tolerance Test (OGTT) needed?
at least 8 hours
15
Finish drinking glucose within ______
5 minutes
16
how many grams of glucose load needed for an adult?
75g
17
The patient shouls vomit after drinking glucose load
False
18
Methods used for IVGTT
Janney-Isaacson
19
Used for diagnosis except in patients with hemoglobinopathies or abnormal RBC turnover
HbA1C
20
Standare method for HbA1C
HPLC-gold standard method
21
Types of Oxidation Reduction
Folin Wu, Nelson Somogyi, Neocuproine, Benedict’s Method
22
Type or Condensation
Orthotoluidine
23
Principle: Cupric ions reduced to cuprous ions forming cuprous oxide in hot alkaline solution by glucose
Cupric Ion Reduction
24
Principle: Inverse colorimetry
Hagedorn Jensen
25
Reagent used for Hagedorn Jensen/Ferric reduction
Hot alkaline solution of potassium ferricyanide
26
Principle: Protein precipitation
Ortho-toluidine
27
End color of ortho-toluidine at 630 nm
Green
28
Interfering substance used in ortho-toluidine
Galactose and Mannose
29
Types of glucose oxidase
Colorimetric , Polarographic
30
Types of Enzymatic
Glucose Oxidase, Hexokinase, Glucose Dehydrogenase
31
Principle: Uses TRINDER REACTION
Colorimetric-Saifer Gernstenfield
32
Chromogens used colorimetric-saifer gernstenfield
3-methyl-2-benzothiazolinone hydrazine, N-N-dimethylaniline, O-dianisidine, Phenol
33
Give the intefering substances of colorimteric-saifer gernstenfield
Ascorbic Acid , Glutathione , Uric Acid, Bilirubin, Hemoglobin, Creatinine, Dopamine , Methyldopa, Citric Acid
34
Principle: Measures oxygen consumption using an electrode. Oxygen depletion is DIRECTLY PROPORTIONAL to the amount of glucose present.
Polarographic Method
35
H2O2 is removed by two additional reactions called:
Catalase , Molybdate
36
Principle: Coupling reaction using G6PD (highly specific) and is measured by quantitating reduced NADPH formation at 340 nm
Hexokinase Method
37
Reference method / Most specific glucose method
Hexokinase Method
38
Excellent for glucose determination
Hexokinase Method
39
Principle: Glucose is reduced to produce a chromophore that is measured spectrophotometrically or an electrical current. The amount of NADPH generated is PROPORTIONAL to the glucose determination.
Glucose Dehydrogenase
40
It covers alpha glucose to beta glucose
Mutarotase
41
Values for fasting plasma glucose
70 to 110 mg/dL
42
Critical / panic values
<40 mg/dL, >500 mg/dL