are fungal diseases that affect subcutaneous tissue

is a chronic, subcutaneous mycosis with eventual lymphatic involvement

▪Historically, the only known species responsible for sporotrichosis was

it may become a generalized infection and involves bones, joints, and other internal organs

▪Sporothrix mode of entry

(Sporotrichosis) Most cases are associated with gardening, particularly with exposure to

Stereotype of the patient at risk for sporotrichosis is the (Sporotrichosis)

is the infective stage of the fungus to man

Disease produced is usually a localized systemic infection

Most commonly seen presentation (Sporotrichosis)

Incubation period of Lymphocutaneous sporotrichosis

A nodule or pustule forms, which may break down into a small ulcer

characterized by involvement of lymphatics from the draining area

▪Chain of lymphatic nodules develops-

Nodules soften and ulcerate, and are connected by

A thin purulent discharge may come from the primary lesion and the earliest lymphatic nodules

(disease becomes chronic) the regional lymph nodes become swollen and may break down

Less commonly seen disease state, in which the infection is confined to the site of inoculation

Lesions may be (Fixed cutaneous sporotrichosis)

Less commonly, there may be infiltrated plaques or red scaly patches

Relatively rare (SPOROTRICHOSIS)

Lesions in (Mucocutaneous sporotrichosis)

At first erythematous, ulcerative and suppurative at first Eventually become granulomatous, vegetative or papilloma-like

Less common systemic form probably follows inhalation

lesions in (Pulmonary sporotrichosis)

Single cavitary lesion of the upper lobe called______ is the most distinctive feature

residual fibrocaseous nodule

chronic pneumonitis with thin-walled cavities with fibrosis and pleural effusion may develop

(SPOROTRICHOSIS) Specimen of Choice

Mold Phase of Sporothrix schenckii (Culture Media)

Also grows well on (Mold Phase of Sporothrix schenckii)

Colonies grow in about (Mold Phase of Sporothrix schenckii)

Mold colony morphology ( Sporothrix schenckii)

color (Mold colony morphology; Sporothrix schenckii)

arranged as a rosette around the apex of a conidiophore

Arranged sympodially around an expanded vesicle at the tip of the conidiophore (Sporothrix schenckii)

identify

Borne on conidiophores that arise at right angles from thin, delicate hyphae

identify

identify

identify

May be observed more than rosettes in (Mold Phase of Sporothrix schenckii)

Microscopic Characteristics (Mold Phase of Sporothrix schenckii)

Account for a dematiaceous appearance of the fungus in culture

Conversion of the mold phase to yeast is accomplished by the following: (Mold Phase of Sporothrix schenckii)

Temperature in conversion of the mold phase to yeast: (Mold Phase of Sporothrix schenckii)

Histopathological examination of tissue sections stained with (Yeast Phase of Sporothrix schenckii)

Yeast Phase of Sporothrix schenckii (wet mount)

(Yeast Phase of Sporothrix schenckii) resembling; diameter

Histologic responses to Sporothrix include

Wet Mount (Yeast Phase of Sporothrix schenckii) percentage

Colony Characteristics (Yeast Phase of Sporothrix schenckii)

Phenotypic characteristics Growth at (SPOROTRICHOSIS)

Phenotypic characteristics Assimilation of (SPOROTRICHOSIS)

A slide latex agglutination test called_____ reliable, sensitive and specific test

Treatment For cutaneous infection

For lymphocutaneous infection

Treatment For disseminated infection drug of choice

identify

Verrucous dermatitidis, chromomycosis is a synonym for

a chronic mycosis of the skin and subcutaneous tissue that develops over a period of months or, more commonly, year

most common species causing Chromomycosis

second most common causing Chromomycosis

Less commonly causing Chromomycosis

Found most commonly in men who have direct contact with soil

Not spread from man to man, and other animals do not contract it

(CHROMOBLASTOMYCOSIS) Little is known about predisposing factors, except

(CHROMOBLASTOMYCOSIS) Characterized by the presence of large, muriform, thick-walled dematiaceous cells called

(CHROMOBLASTOMYCOSIS) Distinguished by the formation of ______ at the site of inoculation

(CHROMOBLASTOMYCOSIS) Longstanding lesions have a

(CHROMOBLASTOMYCOSIS) Mostly asymptomatic in the absence of secondary complications, such as

Sporotrichosis or rose gardener’s disease. Recurrent infections results in fibrosis with scar formation causing lymphatic obstruction, resembling obstruction, resembling

(CHROMOBLASTOMYCOSIS)Lesions are usually confined to the extremities- result of trauma to these areas

(CHROMOBLASTOMYCOSIS) reproduce by dividing in various planes, resulting in multicellular forms

Brown, round, non-hyphal sclerotic bodies, which are non-budding structures occurring singly or in clusters, are seen in tissues

identify

identify

Chromoblastomycosis. H&E stained section showing characteristic dark brown sclerotic cells which divide by

(Chromoblastomycosis) Fission bodies are always color:

are diagnostic for this disease, but do not provide a clue as to species identification of the mold

lead to excessive proliferation of host tissue

This feature differentiates it from-- which result in tissue necrosis (CHROMOBLASTOMYCOSIS)

Sclerotic bodies are usually clumped together, and some may contain a septum; hyphae are not seen

Fungi are seen as round, thick-walled, dematiaceous cells, in diameter (Sclerotic bodies of Chromoblastomycosis)

Fission bodies do not produce buds, thus not considered to be yeast cells

Infected skin is cultured into (Chromoblastomycosis)

Incubate at what temperature (Culture; Chromoblastomycosis)

(CHROMOBLASTOMYCOSIS) Most etiologic agents will not grow at temperatures higher than

(Chromoblastomycosis; Culture) All organisms requiring-- of incubation

(Chromoblastomycosis) Colonies are always what color on the surface and what color on the undersurface?

Colonies are usually heavily furrowed and some species produce a very heaped-up effect