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(CHROMOBLASTOMYCOSIS)Lesions are usually confined to the extremities- result of trauma to these areas
feet and lower legs
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lesions in (Pulmonary sporotrichosis)
joints, meninges and skin
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are fungal diseases that affect subcutaneous tissue
Subcutaneous mycoses
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(Yeast Phase of Sporothrix schenckii) resembling; diameter
cigars 2 to 6 μm in diameter with narrow-based budding
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Less common systemic form probably follows inhalation
Pulmonary sporotrichosis
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Relatively rare (SPOROTRICHOSIS)
Mucocutaneous sporotrichosis
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Single cavitary lesion of the upper lobe called______ is the most distinctive feature
sporotrichoma
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Treatment For disseminated infection drug of choice
amphotericin B
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Sclerotic bodies are usually clumped together, and some may contain a septum; hyphae are not seen
Chromoblastomycosis
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Incubate at what temperature (Culture; Chromoblastomycosis)
25°C to 30°C
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are diagnostic for this disease, but do not provide a clue as to species identification of the mold
Sclerotic bodies of Chromoblastomycosis
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▪Chain of lymphatic nodules develops-
Sporotrichoid
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(disease becomes chronic) the regional lymph nodes become swollen and may break down
Lymphocutaneous sporotrichosis
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▪Sporothrix mode of entry
traumatic implantation
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Conversion of the mold phase to yeast is accomplished by the following: (Mold Phase of Sporothrix schenckii)
(Inoculation) BHIA supplemented with sheep’s blood or on chocolate agar
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Less commonly seen disease state, in which the infection is confined to the site of inoculation
Fixed cutaneous sporotrichosis
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is the infective stage of the fungus to man
Conidium
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Lesions in (Mucocutaneous sporotrichosis)
mouth, pharynx, vocal cords or nose
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For lymphocutaneous infection
itraconazole
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is a chronic, subcutaneous mycosis with eventual lymphatic involvement
Sporotrichosis
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Lesions may be (Fixed cutaneous sporotrichosis)
acneiform, nodular, ulcerated or verrucous
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Nodules soften and ulcerate, and are connected by
tender lymphatic cords
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Microscopic Characteristics (Mold Phase of Sporothrix schenckii)
Thick-walled, dark, sessile conidia
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identify
Chromoblastomycosis. Sclerotic cells on a potassium hydroxide (KOH) preparation
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(SPOROTRICHOSIS) Specimen of Choice
▪Aspirate ▪Curetting ▪Biopsy of the skin lesion ▪Exudates from unopened subcutaneous nodules or from open, draining lesions
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it may become a generalized infection and involves bones, joints, and other internal organs
Sporotrichosis
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Less commonly, there may be infiltrated plaques or red scaly patches
Fixed cutaneous sporotrichosis
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(Chromoblastomycosis; Culture) All organisms requiring-- of incubation
2 to 3 weeks
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identify
Sporothrix schenckii
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A slide latex agglutination test called_____ reliable, sensitive and specific test
yeast cell agglutination test
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Phenotypic characteristics Growth at (SPOROTRICHOSIS)
30°C, 35°C, and 37°C
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identify
Yeast Phase of Sporothrix schenckii- cigar-shaped
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second most common causing Chromomycosis
Phialophora verrucosa
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residual fibrocaseous nodule
sporotrichoma
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(CHROMOBLASTOMYCOSIS) Little is known about predisposing factors, except
trauma and contact with soil
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Infected skin is cultured into (Chromoblastomycosis)
SDA with antibiotics
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Colonies are usually heavily furrowed and some species produce a very heaped-up effect
Chromoblastomycosis
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Colony Characteristics (Yeast Phase of Sporothrix schenckii)
pasty and grayish
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Wet Mount (Yeast Phase of Sporothrix schenckii) percentage
10-20% KOH or calcofluor white
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(CHROMOBLASTOMYCOSIS) reproduce by dividing in various planes, resulting in multicellular forms
Sclerotic bodies (or fission bodies)
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Verrucous dermatitidis, chromomycosis is a synonym for
Chromoblastomycosis
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Sporotrichosis or rose gardener’s disease. Recurrent infections results in fibrosis with scar formation causing lymphatic obstruction, resembling obstruction, resembling
elephantiasis
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(CHROMOBLASTOMYCOSIS) Characterized by the presence of large, muriform, thick-walled dematiaceous cells called
sclerotic bodies (medlar bodies, fission bodies, or “copper pennies”)
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▪Historically, the only known species responsible for sporotrichosis was
Sporothrix schenckii
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color (Mold colony morphology; Sporothrix schenckii)
irregularly dark brown or black
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identify
Mold Phase of Sporothrix schenckii- 'rosettes'
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(CHROMOBLASTOMYCOSIS) Longstanding lesions have a
cauliflower-like surface
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(CHROMOBLASTOMYCOSIS) Mostly asymptomatic in the absence of secondary complications, such as
bacterial infections, carcinomatous degeneration, and elephantiasis
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A nodule or pustule forms, which may break down into a small ulcer
Lymphocutaneous sporotrichosis
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arranged as a rosette around the apex of a conidiophore
Mold Phase of Sporothrix schenckii
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Not spread from man to man, and other animals do not contract it
Chromoblastomycosis
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Fission bodies do not produce buds, thus not considered to be yeast cells
Chromoblastomycosis
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Borne on conidiophores that arise at right angles from thin, delicate hyphae
Mold Phase of Sporothrix schenckii
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Less commonly causing Chromomycosis
Cladophialophora carrionii and Rhinocladiella aquaspersa
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Colonies grow in about (Mold Phase of Sporothrix schenckii)
5 to 10 days
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Mold colony morphology ( Sporothrix schenckii)
membranous and coarsely matted, wrinkled; becoming leathery
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a chronic mycosis of the skin and subcutaneous tissue that develops over a period of months or, more commonly, year
Chromoblastomycosis
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At first erythematous, ulcerative and suppurative at first Eventually become granulomatous, vegetative or papilloma-like
Mucocutaneous sporotrichosis
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identify
(Chromoblastomycosis) dark-brown, round sclerotic body: resembling "copper penny" (H&E stain)
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most common species causing Chromomycosis
Fonsecaea pedrosoi
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Fungi are seen as round, thick-walled, dematiaceous cells, in diameter (Sclerotic bodies of Chromoblastomycosis)
5 to 15 μm
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Phenotypic characteristics Assimilation of (SPOROTRICHOSIS)
sucrose, raffinose, and ribitol
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Yeast Phase of Sporothrix schenckii (wet mount)
KOH preparation &Calcofluor white preparation
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Brown, round, non-hyphal sclerotic bodies, which are non-budding structures occurring singly or in clusters, are seen in tissues
Chromoblastomycosis
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chronic pneumonitis with thin-walled cavities with fibrosis and pleural effusion may develop
Pulmonary sporotrichosis
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Most commonly seen presentation (Sporotrichosis)
Lymphocutaneous sporotrichosis
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Account for a dematiaceous appearance of the fungus in culture
Mold Phase of Sporothrix schenckii
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lead to excessive proliferation of host tissue
Chromoblastomycosis
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Arranged sympodially around an expanded vesicle at the tip of the conidiophore (Sporothrix schenckii)
floret arrangement
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Histopathological examination of tissue sections stained with (Yeast Phase of Sporothrix schenckii)
Periodic Acid-Schiff (PAS) or Gomori methenamine silver (GMS) stains
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(CHROMOBLASTOMYCOSIS) Most etiologic agents will not grow at temperatures higher than
30°C
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Found most commonly in men who have direct contact with soil
Chromoblastomycosis
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This feature differentiates it from-- which result in tissue necrosis (CHROMOBLASTOMYCOSIS)
mycetoma and phaeohyphomycosis
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Stereotype of the patient at risk for sporotrichosis is the (Sporotrichosis)
alcoholic rose gardener
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Mold Phase of Sporothrix schenckii (Culture Media)
Sabouraud dextrose agar with cycloheximide
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identify
mycelial form of Sporothrix schenckii when grown on Sabouraud's dextrose agar at 25oC.
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(CHROMOBLASTOMYCOSIS) Distinguished by the formation of ______ at the site of inoculation
painless verrucous plaque or nodule
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Also grows well on (Mold Phase of Sporothrix schenckii)
Mycosel, Mycobiotic, inhibitory mold agar
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(Chromoblastomycosis) Colonies are always what color on the surface and what color on the undersurface?
dark brown to black and dark black
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characterized by involvement of lymphatics from the draining area
Lymphocutaneous sporotrichosis
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Chromoblastomycosis. H&E stained section showing characteristic dark brown sclerotic cells which divide by
binary fission
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Disease produced is usually a localized systemic infection
Sporotrichosis
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Incubation period of Lymphocutaneous sporotrichosis
8 to 30 days
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Treatment For cutaneous infection
potassium iodide
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(Chromoblastomycosis) Fission bodies are always color:
Brown
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Temperature in conversion of the mold phase to yeast: (Mold Phase of Sporothrix schenckii)
37°C in an atmosphere of 5% CO2
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May be observed more than rosettes in (Mold Phase of Sporothrix schenckii)
mature cultures
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(Sporotrichosis) Most cases are associated with gardening, particularly with exposure to
rose thorns and sphagnum moss
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identify
Chromoblastomycosis
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A thin purulent discharge may come from the primary lesion and the earliest lymphatic nodules
Lymphocutaneous sporotrichosis
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Histologic responses to Sporothrix include
suppurative (including eosinophils) and granulomatous inflammation