SPOROCIDAL

NON-SPOROCIDAL

TISSUE/ BLOOD VESSEL

MUCOUS MEMBRANE FOR WHAT MATERIALS?

MATERIALS SKIN

Action Sterilant is for what materials?

Action High disinfectant

Action intermediate to low-level disinfectant is for what materials?

High level disinfectant = _________ SPOROCIDAL/ non-SPOROCIDAL?

= tuberculocidal l but not sporicidal

what level = not tuberculocidal and sporicidal

. – these two is for disinfection not sterilization

Pasteurization: Batch – _________

Pasteurization: ? – 63% FOR 30 MINS

Pasteurization: Flash _________

Pasteurization: ? 72 DEGREES FOR 15 SECS.

Boiling: _________ for 10-15min

Boiling: 100 DEGCREES CELCIUS for

: 100 DEGCREES CELCIUS for 10-15min

– long wavelength low energy UV light

– are chemical agents applied inanimate objects.

- disinfectant used on living tissue

-70% ethyl/isopropyl alcohol (non sporicidal)

Alcohol -ex? (non sporicidal)

– 2 most EFFECTIVE alcohols

_________% is optimal; EFFECTIVE: EVAPORATE

THESE TWO USES Both antiseptic and disinfectant

BEST ANTISEPTIC HALOGEN

Two Forms OF HALOGEN

TINCTURE:

: IODINE + ALCOHOL

: IODINE + BLEACH/SURFACTANT

IODOPHORE:

- iodine and alcohol (1 to 2 min contact time)

- iodine and alcohol (1 to 2 min contact time)

- iodine and alcohol (1 to 2 min contact time)

- iodine and alcohol (1 to 2 min contact time)

- iodine and alcohol (1 to 2 min contact time)

Betadine- ? (1 to 2 min contact time)

Betadine- iodine and alcohol (? contact time)

antiseptic technique for blood culture:

Chlorine – _________and most commonly used disinfectant

– OLDEST and most commonly used disinfectant

most common use is disinfection of WATER

– BEST DISENFECTANT

Sodium Hypochlorite RATIO

Sodium Hypochlorite CDC RECOMMENDED RATIO

For disinfecting lab SINK

Heavy metal – (2)

(1% eye drop solution)

FOR GONOCOCCAL CONJUCTIVITIS

SLOW BACTERICIDAL

inactivated by blood

reduced by hard water and soap

RESISTANT TO QUATZ AND BENZALKONIUM CHLORIDE

P.aeruginosa RESISTANT TO

STANDARD disinfectant

used in stable and biodegradable. Active in the presence of organic material

– Topical antiseptic. Rapid inactivation of LIPID VIRUS

severe skin infection (skin irritant)

Persistence – major attribute STAYS FOR 6 HOURS

–assoc. to severe toxic effect -> death

: great at MTB but poor in Fungi -Overuse leads to CROSS-RESISTANCE OR CORESISTANCE TO OTHER ANTIMICROBIALS

-destruction of all forms of life

-most common method used for the elimination of microorganisms

Moist Heat – Most effective method (autoclaveat?:

– Most effective method (autoclaveat?: 121 @15 psi for 15 mins

Moist Heat – Most effective method (autoclaveat: 121 @15 psi for 15 mins) Biologic indicator :

Shorter time than dry heat sterilization

Method of Choice for HEAT-_________ Objects

Dry heat a. Temp:

Dry heat a. Temp: 160 – 180C for 1 ½ to 2 hours b. Biologic indicator:

For Heat Stable substances THAT ARE COVERED BY OIL WHICH CANNOT PENETRATE BY WATER

a. Temp: 160 – 180C for 1 ½ to 2 hours b. Biologic indicator:

– alternate heating

Tyndallization:

: 100oC for 30 min for 3 days

Tyndallization: 100oC for 30 min for 3 days i. Day 1: ii. Day 2: iii. Day 3:

Day ?: VEGETATIVE SPORES

Day ?: DESTROY SPORES

Day ?: DESTROY REMAINING CELLS

- for culture media w/PROTEIN

Inspissation TEMP

TEMP 75-80o CELSIUS 2 HOURS FOR 3 DAYS

Incineration – most common method for treating infection_________.

– most common method for treating infection WASTE.

SAFEST method to ensure no infective materials remain.

– plastic syringes, catheter or gloves. Short wavelength, high energy gamma rays

Ionizing radiation . Biologic indicator:

sterilization of disposable supplies such as syringes, catheters, and gloves

– for culture media that cannot be heated;

BEST STERILIXATION METHOD FOR ANTIBIOTIC / VACCINATION

. Filtration – for culture media that cannot be heated; a. Liquid: used

_________ - um (microliter) used for Most bacteria, yeasts, and molds but not Pseudomonas-like or

_________- size for parenteral solutions

Air: HEPA (high-efficiency particulate air) Filter: removes org. _________

Air:? Filter: removes org. > .03