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1
Division of bacterial cells occurs mainly through
Binary fission
2
Process of binary fission
Parent cell enlarges, duplicates its chromosomes, and forms a central transverse septum dividing the cell into two daughter cells
3
Long process of binary fission
1. The parent cell begins as a single cell containing one chromosome, undergoing preparations for the division. 2. Cell enlargement and chromosome replication. ■ The parent cell increases in size and volume, and duplicates its genetic material. ■ The cell also synthesizes necessary structures in preparation for the daughter cell that causes cell enlargement. 3. Chromosome division and septation. ■ A developing central transverse septum begins to wall-off the new cells. 4. Completion of cell compartments. ■ The septum is already completely synthesized through the center. ■ The cell membrane detaches itself forming two separate cell chambers and two chromosomes, each in each chamber. 5. Separation of two daughter cells. ■ The daughter cells are now independent units.
4
Is the process of forming a cross wall between 2 daughter cells
Septation
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Is an anchor that connects the FtsZ ring to the cytoplasmic membrane and stabilizes it (for stabilization)
ZipA
6
Is a protein related to the Actin, an important cytoskeletal protein in eukaryotes
FtsA
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FtsA is a protein related to
Actin
8
Penicillin-binding protein
FtsI
9
Forms a helix of filaments around the inside of the cell wall, just below the cytoplasmic membrane
MreB
10
Localizes the synthesis of new cell wall to specific locations along the long axis of a rod-shaped cell during growth, which allows new cell walls to form at several points along the cell rather than from a single location at your FtsZ site (encircled) outward.
MreB
11
Is a vibrio-shaped species of Proteobacteria, that produces a shape-determining protein called crescentin in addition to MreB.
Caulobacter crescentus
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The protein Caulobacter crescentus produce
Crescentin
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Shape of Caulobacter crescentus
Vibrio shaped
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The number of your microbial cells doubles in a constant time interval.
Exponential growth
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Phases of bacterial growth curve
Lag phase, Log (exponential) phase, Stationary phase, Death phase
16
An organism in an enclosed vessel cannot grow exponentially indefinitely, suggesting that the bacterial growth curve presented above can only occur in bacteria given the provided setting of
Restrained (enclosed) environment
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This phase is characterized by vigorous metabolic activity of cells yet no division is observed or happening, hence zero (0) growth rate.
Lag phase
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This phase can last for minutes up to hours.
Lag phase
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This phase is characterized by rapid cell division.
Log (exponential) phase
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β-Lactam antibiotics, which are bactericidal agents that interrupt bacterial cell-wall formation, are most effective in this phase.
Log (exponential) phase
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This phase is characterized by zero (0) growth rate due to lack of nutrition by depletion.
Stationary phase
22
This is also when toxic products cause growth to slow until the number of new cells produced balances or equal the number of cells that died (new cells = # of cells that died).
Stationary phase
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In addition, bacterial spores are produced in this phase by bacteria like Bacillus and Clostridium.
Stationary phase
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This phase is characterized by a marked decline in the number of viable bacteria.
Stationary phase
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In this phase, the growth rate is negative.
Death phase
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Temperature requirement of Bacteria
Minimum temperature, Maximum temperature, Optimum temperature
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This is the lowest temperature that permits a microbe's growth and metabolism.
Minimum temperature
28
This is the highest temperature that permits a microbe's growth and metabolism.
Maximum temperature
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This is the temperature that promotes the fastest rate of growth and metabolism of a microbe.
Optimum temperature
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Optimum temp - between -5 to 15° C
Psychrophile
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Usually found in environments like arctic and antarctic regions
Psychrophile
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Psychrophile are usually found in
Antarctic and arctic regions
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Temperature psychrophile can tolerate
-5°C - 15°C
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Example of Psychrophile
Polaromonas vacuolata, pschrobacter immobilis
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Optimum temp - between 20 to 30°C but grows well at lower temp
Psychrotrophs
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Important cause of food spoilage
Psychrotrophs
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Temperature psychrotrophs can tolerate
20°C - 30°C
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Optimum temp - between 20 to 30°C; Grows best at 30 to 37°C
Mesophile
39
Most organisms are categorize under this temperature
Mesophile
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Example of Mesophile
Escherichia coli
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Optimum temp - 50 to 60°C
Thermophile
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Temperature thermophile can tolerate
50°C - 60°C
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Example of Thermophile
Geobacillus stearothermophilus
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Optimum temp - Can grow well above the temperature of boiling water which exists under high pressure in the depths of the ocean
Hyperthermophile
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Temperature hyperthermophile can tolerate
Greater than boiling point
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Example of Hyperthermophile
Pyrolobus fumarii, thermococcus celer
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Temperature that is optimal for many free living forms
30°C
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Oxygen requirement for bacteria
Obligate aerobe, Facultative aerobe, Obligate Anaerobe, Aerotolerant, Microaerobe
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Require oxygen to grow and replicate
Aerobes
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Oxygen is harmful to them or they only use little oxygen
Anaerobe
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(+) O2 required
Obligate aerobe
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Example of Obligate aerobe
Nocardia; Bacillus cereus; Neisseria; Pseudomonas; Mycobacterium; Leptospira; Mycoplasma pneumoniae
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(-)/(+) O2 not required but they grow better with O2
Facultative aerobe
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Example of Faculltative aerobe
Staphylococcus; Bacillus anthracis; Corynebacterium; Listeria
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(-) O2 not required and its presence is harmful or lethal to the bacteria
Obligate anaerobe
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Example of Obligate Anaerobe
Actinomyces, Bacteroides, Clostridium
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Under anaerobes, (-) O2 not required and not utilized
Aerotolerant
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Example of Aerotolerant
Propionibacterium; Lactobacillus
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(-) O2 required is only at levels lower than atmospheric pressure
Microaerophile
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Required level is less than 0.2 atm
Microaerophile
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Example of Microaerophile
Streptococcus; Spirochetes; Campylobacter; Helicobacter
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Required pH of Bacteria
Acidophiles, Neutrophiles, Alkalophiles
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Grows between pH 0 and 5.5 (pH < 5.5)
Acidophiles
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Grows between pH 5.5 and 8
Neutrophiles
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Grows between pH 8 and 11.5
Alkalophiles
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pH Acidophiles have
0 - 5.5 pH
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pH Neutrophiles have
5.5 - 8 pH
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pH Alkalophiles have
8 - 11.5 pH
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Example of Acidophiles
Rhodopila globiformis; Acidithiobacillus ferrooxidans
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Example of Neutrophiles
Escherichia coli
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Example of Alkalophiles
Bacillus firmus; Chloroflexus aurantiacus
72
Is the solvent of life and an important factor affecting the growth of microorganisms
Water
73
Require osmolarity of Bacteria
Halophiles, Halotolerants, Osmophiles, Xerophiles
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Microorganisms that inhabit marine environments that have a NaCl requirement and typically grow optimally at the water activity of seawater.
Halophiles
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Microorganisms that can tolerate some level of dissolved solutes but grow best in the absence of the added solute.
Halotolerants
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Organisms that are able to grow in high sugar environments.
Osmophiles
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Organisms that are able to grow in very dry environments.
Xerophiles
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Require nutrition of bacteria
Macronutrients, Micronutrients, Organic, Inorganic
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Required in large quantities; play principal roles in cell structures and metabolism.
Macronutrients
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Required in small amounts; involved in enzyme function and maintenance of protein structure.
Micronutrients
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Contain carbon and hydrogen atoms and are usually the products of living things.
Organic
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Atom or molecule that contains a combination of atoms other than carbon and hydrogen
Inorganic
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Example of Macronutrients
Protein, Lipids, Carbohydrate
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Example of Micronutrients
Manganese, Zinc, Nickel, Selenium
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Example of Organic nutrients
Methane, Carbohydrates, Lipids, Proteins, Nucleic acids