記憶度
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1
The Increase in the population of cells is called
Culture.
2
Cell division is by an asexual process called
Binary fission.
3
The time it takes to divide (double) is called
Generation time.
4
What are the four growth phases
Lag phase, Logarithmic phase, Stationary phase, Decline phase.
5
Organisms are adjusting to the environment (little or no division).
Lag phase.
6
They are synthesizing DNA, ribosomes and enzymes to breakdown nutrients, and to be used for growth.
Lag phase.
7
Division is at a constant rate (generation time) but varies with species, temperature and media.
Logarithmic phase.
8
Cells are most susceptible to inhibitors.
Logarithmic phase.
9
Dying and dividing organisms are at an equilibrium.
Stationary phase.
10
Death is due to reduced nutrients, pH changes, toxic waste and reduced oxygen. Cells are smaller and have fewer ribosomes.
Stationary phase.
11
In some cases cells do not die but they are not multiplying.
Stationary phase.
12
The population is dying in a geometric fashion so there are more deaths than new cells.
Decline phase.
13
Deaths are due to the factors in stationary phase in addition to lytic enzymes that are released when bacteria lyse.
Decline phase.
14
In the (8) give at least first 4 Factor that affect microbial growth
Availability of nutrients, moisture, temperature, ph.
15
In the (7) give the 5-7 that affect microbial growth.
Osmotic pressure, barometric pressure, gaseous Atmosphere.
16
All living organisms require nutrients – various chemical compound use to sustain life.
Availability of nutrients.
17
What are the Sources of basic elements in the availability of nutrients.
C,O,N,H,P,S.
18
What are the other growth factors are organized into three categories:
Purines & pyrimidines, amino acids, vitamins.
19
Required for synthesis of nucleic acids (DNA and RNA)
Purines & pyrimidines.
20
required for the synthesis of proteins.
Amino acids.
21
Needed as coenzymes and functional groups of certain enzymes
Vitamins.
22
What are the terms relating to energy source
Phototrophs & chemotrophs
23
What are the Terms Relating To Carbon Source
Autotrophs & heterotrophs.
24
Cells are composed of how many % of water?
70-95%
25
All living organisms require water to carry out their normal metabolic processes, and most will die in environments containing too little moisture. (True/False)
True.
26
complete drying process.
Dessication.
27
Some microbial stage can survive desiccation. (True/False)
True.
28
The temperature at which the microorganisms grows best.
Optimum growth temperature.
29
Below this, the microorganisms cease to grow
Minimum growth temperature.
30
Above this, the microorganisms die
Maximum growth temperature.
31
microorganisms that grows at temperature of 50-60°C
Thermophiles.
32
microorganisms that grow best at moderate temperature of 20-40°C
Mesophiles.
33
Prefer cold temperature of 10-20°C .
Psychrophiles.
34
OGT is 4°C (refrigerator temperature)
Psychrotrophs.
35
Can tolerate or endure very cold temperature of less than –4°C
Psychrodurics.
36
This refers to the hydrogen ion concentration of a solution and, thus, the acidity or alkalinity of the solution
PH.
37
Most microorganisms prefer a neutral or slightly alkaline growth medium (pH 7.0–7.4). (True/False)
True.
38
the pressure that is exerted on a cell membrane by solutions both inside and outside the cell.
Osmotic pressure.
39
Most bacteria are not affected by minor changes in some thrive at normal atmospheric pressure.
Barometric pressure.
40
Glucose is completely oxidized to CO2 and H2O, requiring 21% oxygen.
Obligate aerobe.
41
The metabolic process is comparable to obligate aerobes but requires 1-15 % oxygen.
Microaerophilic.
42
The absence of O2, glucose undergoes glycolysis to pyruvic acid, and then fermentation takes place.
Facultative anaerobe.
43
Glucose undergoes glycolysis to pyruvic acid, then fermentation or anaerobic respiration in which oxygen is not the final electron acceptor
Obligate anaerobe.
44
Requires carbon dioxide to survive.
Capnophile.
45
What are the classification of culture media.
Consistency, composition, function & use.
46
are used for growth of pure batch cultures.
Liquid media.
47
contain agar and are used widely for the isolation of pure cultures, for estimating viable bacterial populations, and a variety of other purposes.
Solidified media.
48
is one in which the exact chemical composition is known.
Chemically defined medium.
49
is one in which the exact chemical constitution of the medium is not known. Also known as basal medium
Complex medium.
50
contain specific nutrients required for the growth of bacterial pathogens that may be present alone or with other bacterial species in a patient specimen.
Enrichment media.
51
contain nutrients that support growth of most non-fastidious organisms without giving any organism a growth advantage.
Supportive media.
52
contain one or more agents that are inhibitory to all organisms except those being sought
Selective media.
53
He demonstrated that fermentation is caused by microorganisms.
Louis pasteur.
54
He also found that yeast ferments sugar to alcohol and that bacteria can oxidize alcohol to acetic acid.
Louis pasteur.
55
a process used to kill microorganisms responsible for spoilage
Pasteurization.
56
Glucose without oxygen.
Fermentation.
57
Glucos with oxygen
Respiration.
58
The final electron acceptor is an inorganic molecule other than O2
Anaerobic.
59
The total ATP yield is less than aerobic respiration because only part of the Krebs cycle operates under anaerobic conditions. (True/False)
True.
60
the final electron acceptor is an organic molecule.
Fermentation.
61
O2 is not required in fermentation. (True/False)
True.
62
In alcohol fermentation, acetaldehyde is reduced by NADH to produce ethanol.
Fermentation.
63
Heterolactic fermentors can use the pentose phosphate pathway to produce lactic acid and ethanol.
Fermentation.