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問題一覧
1
What is the average blood in human?
5L
2
Composition of blood
55% plasma; 45% formed elements
3
fat, plasma, buffy coat, erythrocytes
4
Treat all specimens such as blood, body fluids, and unfixed tissue to be ______
potentially infectious
5
Most effective way of breaking the chain of infection?
handwashing
6
Handwash at least _____
15 seconds
7
The process of destroying pathogenic microorganisms in inanimate object is?
disinfection
8
Bleach (sodium hypochlorite) dilution is?
1:10
9
all are correct order of draw except:
sts, red top, coagulation tube, EDTA, gray top
10
I. yellow II. orange III. green IV. lavender and pink V. gray which of the ff. needs 8 inversions
I, II, III, IV, V
11
What is the preferred concentration of anticoagulant in oxalate?
1-2 mg/mL of blood
12
What is the preferred concentration of anticoagulant in citrate?
3.2 - 3.8 g/dL
13
What is the preferred concentration of anticoagulant in fluoride?
10 mg/mL of blood
14
What is the preferred concentration of anticoagulant in heparin?
0.2 mg/mL of blood
15
Tourniquet should be applied _____ above the venipuncture site.
3-4 inches
16
_____ is used for sample collection for blood alcohol measurement
Benzalkonium chloride
17
What should be done if the patient refuses the venipuncture procedure?
gently pursuade the patient
18
Skin is performed in: I. pediatric patients II. adults who are severely burned III. pregnant woman IV. elderly patients
I, II, IV
19
Capillary puncture should not be more than _____ deep
2mm
20
Hemoglobin is composed of _____ heme and _____ globin chains
4 and 4
21
what is the hemoglobin count at birth?
15-20 g/dL
22
what is the hemoglobin count of adult women?
12-16 g/dL
23
what is the hemoglobin count of men?
13-18 g/dL
24
It is also known as modified drabkin's reagent
cyanmethemoglobin method
25
to shorten the reaction time in cyanmethemoglobin method, sodium bicarbonate is replaced by
KH2PO4
26
_____ donates cyanide to hemoglobin
potassium cyanide
27
sodium bicarbonate is replaced by KH2PO4 and shortens the reaction time from 15 minutes to _____
3 minutes
28
What wavelength does cyanmethemoglobin method is using?
540nm
29
All are measured in modified drabkins reagent except:
sulfhemoglobin
30
It only measures the functional hemoglobin
Blood Oxygen Capacity
31
Also know as Van Slyke Method
Blood Oxygen Capacity
32
In Blood Oxygen Capacity (Van Slyke Method) 1g of Hb carries _____ of oxygen
1.34
33
Hemoglobin iron content = 100g of Hb = _____ of Fe++
0.347g
34
It is a method used for blood donor screening
copper sulfate method
35
also known as copper sulfate method
gravimetric method
36
If the hemoglobin is equal to or more than _____, the blood will sink in within _____ and the donor is accepted
12.5 g/dL; 15 seconds
37
the specific gravity of the copper sulfate solution is _____
1.053
38
In the copper sulfate method (gravimetric method), the drop of blood should be added from a height of _____
1cm
39
acid hematin is also known as
sahli's method
40
reagent for acid hematin
0.1 N HCl
41
HbF is alkali _____
resistant
42
Destruction of all forms of microbial life including spores
Sterilization
43
It is a combination of hemoglobin with carbon monoxide and unable to transport oxygen
carboxyhemoglobin
44
In carboxyhemoglobin the affinity for carbon monoxide is _____ greater than for oxygen
200x
45
carboxyhemoglobin color and peak absorbance
color: cherry-red peak absorbance: 576nm
46
methemoglobin can cause _____ discoloration of the blood
chocolate brown
47
peak absorbance in methemoglobin
630nm
48
when ferrous ion has been oxidized to ferric state it is called
methemoglobin
49
sulfhemoglobin causes a _____ discoloration of the blood and has a peak absorbance at _____
mauve-lavender; 618nm
50
It is the volume of packed RBCs that occupies a given volume of whole blood
hematocrit
51
hematocrit at birth
45-60%
52
hematocrit in females
36-48%
53
hematocrit in males
40-55%
54
microhematocrit tube is _____ long with an internal bore of _____ and can hold _____ of blood
75mm; 1.2mm; 0.05mL
55
microhematocrit centrifuge is capable of _____ RCF _____ minutes
10,000-15,000; 5
56
K2-EDTA whole blood is more preferred than K3-EDTA whole blood
true
57
In microhematocrit method incomplete sealing will lead to falsely increase results
false
58
In microhematocrit method, inadequate centrifugation will lead to falsely increased result
true
59
In microhematocrit method, allowing the tube to stand longer than several minutes leads to falsely increased result
true
60
overanticoagulation in microhematocrit method will lead to LOW hct and HIGH esr
false
61
Increased amount of trapped plasma is found in: I. macrocytic II. microcytic III. spherocytosis IV. thalassemia V. dacrocytosis VI. hypochromic VII. sickle cell
I, III, IV, VI, VII
62
equipment used in the macrohematocrit method
wintrobe tube
63
length and size of internal bore in wintrobe tube
115mm long; 3mm internal bore
64
rule of three is only applies in _____
normocytic, normochromic
65
normal rbc count for newborns
5.0-6.5x10^12/L
66
normal rbc count for females
3.6-5.6x10^12/L
67
normal rbc count for males
4.2-6.0x10^12/L
68
whole blood is diluted with isotonic diluting fluid to facilitate counting and prevent the lysis of RBCs
true
69
wbc count for adults
4.0-11.0x10^9/L
70
wbc count for newborns
10.0-30.0x10^9/L
71
wbc count for 1 year of age
6.0-17.0x10^9/L
72
wbc count procedure: statement 1: allow the dilution to sit for 10 minutes to ensure that the red blood cells (RBCs) have lysed statement 2: leukocytes counts should be performed within 2 hours of dilution
only statement 1 is correct
73
diluent for wbc counting 1% ammonium oxalate 3% acetic acid 1% HCl
true
74
diluent for rbc counting isotonic saline
true
75
diluent for platelet count 1% ammonium oxalate
true
76
size: 10-15 um nucleus: segmented in 2-5 lobes (2-4 lobes) cytoplasm: stains light pink due to the presence of secondary granules, and purple because of a few primary granules
neutrophil (segmented)
77
size: 10-15 nucleus: sausaged shape, elongated curve cytoplasma: identical to segmented neutrophil
neutrophil (band)
78
size: 12-17 um nucleus: dark purple, band shaped or segmented with only two lobes cytoplasm: contains large, spherical granules that stain orange-pink
eosinophil
79
size: 10-14 um nucleus: light to purple staining, usually difficult to see due to overlying granules, bilobed cytoplasm: densely stained, dark violet granules
basophil
80
size: 12-20 um nucleus: round, horseshoe-shaped or lobulated, usually folded or with convolutions cytoplasm: abundant cytoplasm with gray-blue containing indistinct granules giving it a ground glass apppearance
monocyte
81
size: small (6-8 um), medium to large (8-12 um) nucleus: deep purple, compact, densely packed clumps, may be round or oval, or indented cytoplasm: stains pale to bright sky blue, may contain a few prominent reddish (azurophilic) granules (a.k.a robin's egg blue cytoplasm)
lymphocyte
82
if the differential count shows the presence of immature granulocyte, it is termed _____ and may be found in disorders like leukemia and bacterial infections
shift to the left
83
It refers to an increased number of hypersegmented neutrophils (megaloblastic anemia)
shift to the right
84
It indicates the average volume of RBCs in femtoliters (fL)
MCV
85
An expression of the average concentration of hemoglobin in red blood cells
MCHC
86
It indicates the average weight of hemoglobin in the red blood cells
MCH
87
RDW is
1. determined from the RBC histogram; coefficient of variation of the MCV 2. directly proportional to the variation in RBC size (anisocytosis)
88
_____ is the true increase of MCHC
spherocytosis
89
reference range of MCHC
32-36 g/dL
90
reference range of MCH
28-32 pg
91
reference range of RDW
11.5-14.5%
92
what is the MCV, MCHC and RBC morphology of the following diseases/conditions IDA, thalassemia, sideroblastic anemia, defective iron use, chronic infection/inflammation
MCV: < 80 MCHC: < 32 MORPHOLOGY: microcytic; hypochromic
93
what is the MCV, MCHC and RBC morphology of the following diseases/conditions hemolytic anemia, aplastic anemia, acute blooad loss anemia
MCV: 80-100 MCHC: 32-36 MORPHOLOGY: normocytic; normochromic
94
what is the MCV, MCHC and RBC morphology of the following diseases/conditions liver disease, myelodysplasias, megaloblastic anemia
MCV: > 100 MCHC: 32-36 MORPHOLOGY: macrocytic; normochromic
95
coverslip technique is the most common and most convenient technique for blood smear
false
96
the size of the blood drop in blood smear must be _____
2-3 mm
97
statement 1: a drop of blood that is too large can cause a short and thin smear statement 2: a drop of blood that is too small can cause a short and thick smear
both statement is wrong
98
In manual wedge technique the angle must be _____
30-45°
99
All factors can affect the thickness of a wedge smear except: I. pressure II. viscosity of blood III. angle IV. size of blood V. glass slide VI. speed
II, V