Produce thin sections for microscopy

Bacteria are

Remove a substantial amount of fat from the tissue which otherwise presents a barrier to wax infiltration

Uses scanning technique, fluorochromes, and long-wavelength light to penetrate into deep thick specimens

Create artificial spaces between cells and other tissue

Major types of electron microscopy

It produce ecm

Replace alcohol with a substance that is transparent to allow light to pass through

It holds water and giving the physical scaffolding for the cells

Remove water from the sample to prepare it for emedding

Structures stained purple

Smallest living parts of the body and Basic structural and function units

Uses laser to scan multiple z-planes successively

The ethanol is gradually replaced with xylene

Completely displace the clearing agent.

Connective tissues non cellular portion is known as the

The hardened block with tissue and surrounding embedding medium is trimmed and placed for sectioning in a microtome

Secure the sections onto a microscope slide

Minor shrinkage of cells or tissue produced by the fixative or by ethanol that needed for paraffin embedding

Uses interference patterns to enhance contrast between different features of a specimen to produce high-contrast images

In human the main components of extracellular matrix are

It is the most crucial step for the preperation of tissues it preserved the samples structure and prevent decomposition

Two interacting components of tissues

Provide support and orientation for sectioning

Uses electron beams to visualize surfaces

Interpretations of structures in tissue selections

Increasing contrast without staining by producing a bright image on darker background

Uses electron beams that pass through a specimen to visualize small images

Form part of a selective barrier to the external environment

It involves all aspects of tissue biology with focus on how cells structure and arrangement optimize functions specific to each organ

Study of tissues of the body

Used in a wide variety of laboratory application as the standard microscope

Examples of fixative or liquid fixing agent

Discovered different weaves and textures in the body and named then layers of tissues

Major types of light microscopy

Preperation of tissues for study

Tissue or web

From the stain which may confused with cellular structures such as cytoplasmic granules

Structures stained pink

Animal cells are

May confuse with linear structures in the tissue

Father of modern histology and descriptive anatomy

Hardening of infiltration tissue with paraffin

Types of tissue sections

Uses flourescent stains to produce an image

Uses refraction and interference caused by structures in the specimen to create high-contrast, high-resolution images without staining

First did the study of tissus and used the term tissue in an anatomic sense

Supports the cells and contains fluid transporting nutrients to the cells carying away wastes

Enhance contrast and reveal specific structures. Dyes stain material more or less selectively, often behaving like acid or basic compounds