問題一覧
1
Most commonly used method, aiding aolute molecules lower temperature that freeze
freezing points osmometry
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Migration of charged particles in an electric field. It separates proteins based on their electric densities.
electrophoresis
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separates by molecular size
cellulose acetate
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separates by electrical charge
agarose gel
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separates on the basis of charge and molecular size; separates proteins into 20 fractions used to study isoenzymesseparates
polyacrylamide gel
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it measures the absorbance of stain-concentration of the dye and protein fraction
densitometry
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- sample molecules are separated by electro-osmotic flow - it utilizes nanoliter quantities
capillary electrophoresis
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has a net charge that can either be negative or positivhas a net charge that can either be negative or positive
amphoteric
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migration of small, charged ions
iontophoresis
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the migration of charged macromolecules
zone electrophoresis
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Ionizes molecules, then separates and identifies ions depending on their mass-to-charge ratio (m/z)
mass spectrometry
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It involves doing mass analysis twice
MS2
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It is the separation of soluble components in a solution by specific differences in physical- chemical characteristics of the different constituents, with a mobile phase and a stationary phase.
chromatography
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it is used for the fractionation of sugar and amino acid
paper chromatography
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Sorbent: paper chromatography
whatman paper
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It is a semiquantitative drug screening test
thin layer chromatography
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the gold standard in drug testing for identification and quantitation of drugs in body fluids
GC-MS
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it is the method of choice for thermolabile compounds to achieve better separation of analytes
liquid chromatography
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the most widely used liquid chromatography
hplc
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it uses pressure for fast separations, controlled temperature, in-line detectors, and gradient elution technique
hplc
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Ionized samples are separated based on their mass-to-charge ratio and measured by determining the time it takes for the ions to travel to a detector at the end of a time-of-flight tube.
maldi-tof-ms
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The measurement of current or voltage generated by the activity of a specific ion.
electrochemistry techniques
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measurement of potential (voltage) between two electrodes in a solution to measure analyte concentration
potentiometry
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consists of mercury in contact with a solution that is saturated with mercurous chloride and a known concentration of potassium chloride
saturated calomel electrode
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consists of a silver electrode immersed in potassium chloride solution that has been saturated with silver chloride
silver-silver chloride
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It is very sensitive and measures the activity of one ion
ion selective electrode
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The measurement of the amount of electricity (in coulombs) at a fixed pot
coulometry
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The measurement of the current flow produced by an oxidation reaction
amperometry
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The measurement of current after which a potential is applied to an electrochemical cell
voltammetry