Protection and control for biological materials to prevent their unauthorized access, loss, theft, diversion, or intentional release

Protect the germs from people

Examples of biosecurity

Examples of biosafety

Containment principles and practices to prevent unintentional exposure to pathogens and toxins or their unintentional release

Protect the people from germs

Laboratory response network responsible for definitive characterization of agents

Has the reagents and technology for confirmatory testing of pathogens

LRN that detects and recognizes a possible bioterrorism event. Formerly Level A laboratories

Treat all human blood and certain body fluids as infectious for bloodborne pathogens (hiv, hbv, etc)

Modification of UP- treat all as infectious

Apply only to blood and all body fluid or secretions except sweat, non intact skin and mucous membrane

Highest risk; Easily transmitted; Highly Infectious

Highest risk; Easily transmitted; Highly Infectious

Moderately easy to transmit; Moderate to Low mortality

Moderately easy to transmit; Moderate to Low mortality

Emerging/Recurrent; Could be engineered in the future

Emerging/Recurrent; Could be engineered in the future

For instances in which the agent is highly transmissible like a person under investigation (PUI) or confirmed for ebola virus, an outer apron may not be necessary.

BSL - I risk description

BSL - II risk description

BSL - III risk description

BSL - IV risk description

BSL - I risk description

BSL - II risk description

BSL - III risk description

BSL - IV risk description

Required BSC for BSL-I

Required BSC for BSL-II

Minimum required BSC for BSL-III

Required BSC for BSL-IV

Transmission-based Isolation Recommendations for Contact Precautions (MDR bacterial infections, Adenovirus, Diarrhea and GI infections, Hepa A, Herpes simplex, Lice, Scabies; Mask if with respiratory: Parainfluenza, RSV, Chickenpox)

Transmission-based Isolation Recommendations for Droplet Precautions (Pertussid, Influenza, Neisseria meningitidis is and other bacterial meningitidis, Cocksackie, RSV, mumps, Rubella)

Transmission-based Isolation Recommendations for Airborne precautions (Chicken pox, Shingles, Measles; N95 respirator: TB, SARS, Avian Influenza (H5-H10)

Meaning of HEPA

HEPA filter size and efficiency

No air recirculation; total exhaust to the outside

70% recirculated; 30% exhausted

70% recirculated; 30% exhausted; has 100 LFM

30% recirculated; 70% exhausted

BSC class with Vertical Laminar flow

BSC class for Research facilities

There should be ____ feet around the bsc during operation to ensure the proper air circulating system

Certification of biosafety cabinet should be done after

Spillage

Killing all forms of microbial life including spores

Killing pathogenic microbes but not necessarily all microbes, prions and spores

Removal of pathogenic microbes in inanimate objects so that they are safe to handle and dispose

Autoclave, Tyndalization/Fractional sterilization and Inspissation are types of sterilization method under

Autoclave settings for sterilising broth and media

Autoclave settings for medical waste w/ blood/bloody fluids; Best sterilization and decontamination of used media

Tyndallization/Fractional sterilization is a process that happens for 3 consecutive days. It kills ___ on the 1st day, ___ on the second say, and ___ on the 3rd day.

Recommended sterilization for Lowenstein Jensen

Inspissation settings

For drying glassware, cotton swab, oils, petrolatum, powder

Oven temp and time

For research animals and medical/laboratory waste; safest method to ensure no infective materials remain in samples

Inceneration temperature

It acts as a membrane when pulling liquids with a vacuum in the Membrane Filtration sterilization process

Used in sterilizing disposable such as plastic syringe, vacutainer, blood tubes, catheters and gloves

For heat-sensitively materials; most common gas sterilant

And oxidizing agent: Used in sterilizing HEPA filter in BSCs, metals, and medical instruments like scissors; No toxic by-product

Principle: generates plasma by exciting the gas in enclosed chamber under deep vacuum with the use of radio frequency or microwave energy

Physical methods of disinfection: Boiling

Physical methods of disinfection: Batch method

Physical methods of disinfection: Flash method

Physical methods of disinfection: Bailey's

Properly pasteured milk should have a

Physical disinfection method used in working surfaces of BSC and contaminated rooms

Resistant to Alcohol disinfection

Most common concentration of Sodium hypochlorite (house bleach)

Sodium hypochlorite dilution according to CDC recommended for cleaning tabletop after blood spills

Which of the following can be used for disinfecting BSC hoods when in gas form

Sporicidal in medical equipment in 3-10 hours; does not corrode lens, metal, rubber, bronchoscope

Same as gluteraldehyde but more stable and requires no activation; does not require exposure monitoring and not mucus membrane irritant

Effective even in the presence of organic material; used in surface sterilization of surgical instruments; kills bacteria, fungi and even their spores

Concentration of Hydrogen Peroxide in Catalase test

Concentration of Hydrogen Peroxide in Superoxol test

When dealing with spillage, it is necessary to Adsorp with paper towel first before neutralizing

Used in benchtops or surfaces, floors, furniture and walls. It is activated by organic compounds; non-tuberculoidal, non-sporocidal

Kills bacteria, mycobacteria, virus, fungi. It is not sporicidal

Iodine + alcohol

Iodine + neutral polymer (Povidone-iodine, Poloxamer-iodine)

Contact time for iodophore in drawing blood culture and surgery

Tincture of Iodine and Iodophore are examples of

70% ethyl alcohol followed by iodophor is used in drawing blood culture and surgery

Derivatives of carbolic acid

Stable, biodegradable and active in the presence of organic materials. Can be found in germicidal soap

Has high antimicrobial activity, better than povidone iodine, and has low toxicity. More effective on gram+ than gram-. Less effective in fungi and mycobacteria; baked viruses are not in activated.

For outbreaks of gram+ infections

Halogen-substituted phenolic compound

Added to many household products; clothing kitchenware toothpaste and toys to prevent bacterial contamination

It requires on-site preparation; economical alternative to expensive antiseptics; used in cleaning of chronic wounds prior to administration of antibiotics

A procedure that involves washing a newborn's eyes with a 1% Silver nitrate to prevent ophthalmia neonatorum of N. gonorrheae

Substitute for 1% Silver nitrate in performing Crede's prophylaxis

Bioindicators for Autoclave

Bioindicators for dry heat/oven