暗記メーカー

IMMUNOHISTOCHEMISTRY

IMMUNOHISTOCHEMISTRY

11問 • 1年前

問題一覧

1

Used for identification of specific and highly selective cellular EPITOPES or ANTIGENS in tissues

IMMUNOHISTOCHEMISTRY

2

Immunizing an animal with a purified specific molecule (IMMUNOGEN) Involves collection of immunoglobulin rich serum afterproduction of HUMORAL ANTIBODY against antigen Immunochemically NOT IDENTICAL to each other = CROSS REACT

POLYCLONAL ANTIBODIES

3

products of an individual clone of plasma cells Produced by HYBRIDOMA and CLONING TECHNIQUES Antibody produce are immunochemically identical that DO NOT CROSS REACT Propagation: culture medium or transplantation of hybridoma in MICE

MONOCLONAL ANTIBODY

4

Use of horse radish peroxidase + diaminobenzidine

ENZYME LABELS

5

Use of fluorescein isothiocyante

FLUOROCHROME LABELS

6

to breakdown formalin cross linking, unmask and allow certain antigenic sites to be exposed

PROTEOLYTIC ENZYME DIGESTION

7

Involves boiling of formalin fixed deparaffinized sections in

MICROWAVE ANTIGEN RETRIEVAL

8

Less time consuming More consistent in recovery of many antigens Not subjected to “HOT SPOTS” and “COLD SPOTS”

PRESSURE COOKING ANTIGEN RETRIEVAL

9

Section known and proven to have the antigen in question

POSITIVE CONTROL

10

Omit the primary antibody from the staining schedule Replace the specific primary antibody by an Ig that is directed against unrelated antigen

NEGATIVE CONTROL

11

BUILT IN” control - Eliminates the variable of tissue fixation between specimens and controls - Contains the target antigen but not in the tissue elements under investigation

INTERNAL TISSUE CONTROL

HISTOPATH (lesson 1)

HISTOPATH (lesson 1)

Kmyching · 56問 · 2年前

HISTOPATH (lesson 1)

HISTOPATH (lesson 1)

56問 • 2年前

bacte (microbial control)

bacte (microbial control)

Kmyching · 26問 · 2年前

bacte (microbial control)

bacte (microbial control)

26問 • 2年前

Collection

Collection

Kmyching · 6問 · 2年前

Collection

Collection

6問 • 2年前

Histopath (somatic death)

Histopath (somatic death)

Kmyching · 25問 · 2年前

Histopath (somatic death)

Histopath (somatic death)

25問 • 2年前

fresh tissue

fresh tissue

Kmyching · 54問 · 2年前

fresh tissue

fresh tissue

54問 • 2年前

Staphylococcus

Staphylococcus

Kmyching · 7問 · 2年前

Staphylococcus

Staphylococcus

7問 • 2年前

lesson 1 (cell injury, adaptation and inflammation and repair)

lesson 1 (cell injury, adaptation and inflammation and repair)

Kmyching · 67問 · 2年前

lesson 1 (cell injury, adaptation and inflammation and repair)

lesson 1 (cell injury, adaptation and inflammation and repair)

67問 • 2年前

biopsy

biopsy

Kmyching · 14問 · 2年前

biopsy

biopsy

14問 • 2年前

autopsy

autopsy

Kmyching · 20問 · 2年前

autopsy

autopsy

20問 • 2年前

decalcification

decalcification

Kmyching · 20問 · 2年前

decalcification

decalcification

20問 • 2年前

DEHYDRATION AND CLEARING

DEHYDRATION AND CLEARING

Kmyching · 27問 · 2年前

DEHYDRATION AND CLEARING

DEHYDRATION AND CLEARING

27問 • 2年前

IMPREGNATION AND EMBEDDING

IMPREGNATION AND EMBEDDING

Kmyching · 21問 · 2年前

IMPREGNATION AND EMBEDDING

IMPREGNATION AND EMBEDDING

21問 • 2年前

TRIMMING AND SECTIONING

TRIMMING AND SECTIONING

Kmyching · 35問 · 2年前

TRIMMING AND SECTIONING

TRIMMING AND SECTIONING

35問 • 2年前

adhesive and staining

adhesive and staining

Kmyching · 43問 · 2年前

adhesive and staining

adhesive and staining

43問 • 2年前

COMMON STAINING SOLUTION

COMMON STAINING SOLUTION

Kmyching · 19問 · 2年前

COMMON STAINING SOLUTION

COMMON STAINING SOLUTION

19問 • 2年前

OTHER STAINING

OTHER STAINING

Kmyching · 26問 · 2年前

OTHER STAINING

OTHER STAINING

26問 • 2年前

H&E STAINING, MOUNTING, RINGING AND LABELLING

H&E STAINING, MOUNTING, RINGING AND LABELLING

Kmyching · 19問 · 1年前

H&E STAINING, MOUNTING, RINGING AND LABELLING

H&E STAINING, MOUNTING, RINGING AND LABELLING

19問 • 1年前

EPITHELIAL TUMOR MARKERS

EPITHELIAL TUMOR MARKERS

Kmyching · 5問 · 1年前

EPITHELIAL TUMOR MARKERS

EPITHELIAL TUMOR MARKERS

5問 • 1年前

INTERMEDIATE FILAMENT MARKERS

INTERMEDIATE FILAMENT MARKERS

Kmyching · 6問 · 1年前

INTERMEDIATE FILAMENT MARKERS

INTERMEDIATE FILAMENT MARKERS

6問 • 1年前

NEUROENDOCRINE MARKERS and GERM CELL TUMOR MARKERS

NEUROENDOCRINE MARKERS and GERM CELL TUMOR MARKERS

Kmyching · 6問 · 1年前

NEUROENDOCRINE MARKERS and GERM CELL TUMOR MARKERS

NEUROENDOCRINE MARKERS and GERM CELL TUMOR MARKERS

6問 • 1年前

MESENCHYMAL TUMOR MARKERS

MESENCHYMAL TUMOR MARKERS

Kmyching · 5問 · 1年前

MESENCHYMAL TUMOR MARKERS

MESENCHYMAL TUMOR MARKERS

5問 • 1年前

問題一覧

1

Used for identification of specific and highly selective cellular EPITOPES or ANTIGENS in tissues

IMMUNOHISTOCHEMISTRY

2

Immunizing an animal with a purified specific molecule (IMMUNOGEN) Involves collection of immunoglobulin rich serum afterproduction of HUMORAL ANTIBODY against antigen Immunochemically NOT IDENTICAL to each other = CROSS REACT

POLYCLONAL ANTIBODIES

3

products of an individual clone of plasma cells Produced by HYBRIDOMA and CLONING TECHNIQUES Antibody produce are immunochemically identical that DO NOT CROSS REACT Propagation: culture medium or transplantation of hybridoma in MICE

MONOCLONAL ANTIBODY

4

Use of horse radish peroxidase + diaminobenzidine

ENZYME LABELS

5

Use of fluorescein isothiocyante

FLUOROCHROME LABELS

6

to breakdown formalin cross linking, unmask and allow certain antigenic sites to be exposed

PROTEOLYTIC ENZYME DIGESTION

7

Involves boiling of formalin fixed deparaffinized sections in

MICROWAVE ANTIGEN RETRIEVAL

8

Less time consuming More consistent in recovery of many antigens Not subjected to “HOT SPOTS” and “COLD SPOTS”

PRESSURE COOKING ANTIGEN RETRIEVAL

9

Section known and proven to have the antigen in question

POSITIVE CONTROL

10

Omit the primary antibody from the staining schedule Replace the specific primary antibody by an Ig that is directed against unrelated antigen

NEGATIVE CONTROL

11

BUILT IN” control - Eliminates the variable of tissue fixation between specimens and controls - Contains the target antigen but not in the tissue elements under investigation

INTERNAL TISSUE CONTROL