記憶度
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1
Causative agent of Lyme disease
Borellia (Boreliella)
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Arrangement of bacteria in pairs is also known as
Diplococci
3
Arrangement of bacteria in single is also known as
Monococci
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Arrangement of bacteria is chains is also known as
Streptococci
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Arrangement of bacteria in cluster is also known as
Staphylococci
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8-membered cocci formation of bacteria
Sarcina
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Comparison of the Cytoplasmic membrane of bacteria, eukarya, and archeae
In contrast to the lipids of the bacteria and eukarya in which ester linkages bond fatty acid to glycerol, the lipids of archaea contain either bands between glycerol and its hydrophobic side chains.
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The membrane structure of archaea may be
Bilayer, monolayer, or both
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Are extremely resistant (R) to heat. Therefore, widely distributed among hyperthermophilic archaea
Monolayer
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Organisms that grow best at temperatures above ↑ 80 °C
Hypothermophilic archaea
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All bacteria have a cell wall composed of peptidoglycan except
Mycoplasma
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Petidoglycan is made up of
Sugar backbone (glycan) + peptide side chains (peptido) cross-linked by transpeptidase
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Rigid layer is a polysaccharide composed of 2 sugar derivatives
N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM)
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Rigid layer is also composed of a few amino acids
L-alanine, D-alanine, D-Glutamic Acid, either L-lysine or structural similar molecule Diaminopimelic acid (DAP)
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Facilitate passage of small, hydrophilic molecules into the cell.
Porin proteins
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In the outer membrane of gram-negative bacteria → channels allow the entry of essential substances such as
Sugars, amino acids, vitamins and metals as well as many antimicrobial drugs such as Penicillins
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Example of Gram-negative bacteria
Salmonella, Shigella, E-coli
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Most common cause of Urinary tract infection
E-Coli
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Has small amounts of peptidoglycan in the cell wall
Gram-negative bacteria
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Has several sheets of peptidoglycan stacked from one another
Gram-positive bacteria
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Its cell wall mostly consists of outer membrane called lipopolysaccharide (LPS)
Gram-negative bacteria
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Does not contain outer membrane
Gram-positive bacteria
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Gram staining Mnemonics
V - Violet/Crystal Violet (primary stain) I - Iodine (mordant) A - Alcohol (decolorizer) S - Safranin (secondary stain)
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Has thicker and multilayer peptidoglycan layer
Gram-positive bacteria
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Has thinner peptidoglycan layer
Gram-negative bacteria
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Has teichoic acid in its layer
Gram-positive bacteria
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Has lipopolysaccharide and periplasmic space in its layer
Gram-negative bacteria
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Outer membrane of gram-negative bacteria contains
Endotoxin known as Lipopolysaccharide (LPS)
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All gram positive bacteria have NO ENDOTOXIN EXCEPT FOR
Listeria monocytogenes
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Are absent from the cell walls of Archaea.
Peptidoglycan and an outer membrane
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A variety of cell wall types are found in Archaea including those that contain
Polysaccharides, proteins, glycoproteins
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The backbone of pseudomurein is formed from the alternating repeats of
→ N-acetylglucosamine and → N-acetyltalosaminuronic acid
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Cell wall of some archaea lacks pseudomurein and instead contain other polysaccharides like in cases of methanosarcina sp. they contain
Glucuronic acid, galactosamine, uronic acid, acetate
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Lipoprotein bilayer without sterols
Cytoplasmic membrane
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Site of oxidative and ETC enzymes
Cytoplasmic membrane
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Serves as a permeability barrier
Cytoplasmic membrane
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Prevent passage of leakage of solutes into and out of the cell
Cytoplasmic membrane
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Prevent passage of leakage of solutes into and out of the cell
Cytoplasmic membrane
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The membrane is an anchor for many proteins some of this are enzymes that function in the energy conservation and transport of solutes into and out of the cell
Cytoplasmic membrane
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Major site for energy conservation in the prokaryotic cell via the proton motive force
Cytoplasmic membrane
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RNA and protein in 50s and 30s subunits
Ribosome
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Major function is for protein synthesis
Ribosome
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Invagination of plasma membrane
Mesosome
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Participates in cell division and secretion
Mesosome
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Space between plasma membrane and outer membrane
Periplasm
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Contains many hydrolytic enzyme beta lactamases
Periplasm
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The enzyme periplasm contains
Beta lactamases
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Essential components of bacteria
Cytoplasmic membrane, ribosome, mesosome, periplasm
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Non essential components of bacteria
Glycocalyx, flagella, pili, fimbriae, capsules
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Mediates adherences to surfaces that acts as biofilms
Glycocalyx
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Two types of glycocalyx
Slime layer, capsule
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Loosely organized and attached
Slime layer
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Highly organized and tightly attached
Capsule
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Can be lost from the cell surface
Slime layer
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Adhere firmly to the cell wall and some are even covalently link to the petidoglycan
Capsule
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Common features of slime layer and capsule
→ Assist in the attachment of the bacterial cell to solid surfaces forming biofilms. → Act as virulence factors in certain bacterial diseases → Prevents the cell from dehydration
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Many prokaryotes become motile via swimming through
Flagella
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Parts of the flagella:
Filament, hook, basal body
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Long, thin, helical structure composed of protein flagellin.
Filament
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Curved sheath
Hook
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Stacks of rings firmly anchored in cell wall.
Basal body
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Flagella can rotate
360°
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Responsible for the motility of the cell, and guide the direction of the bacteria in response to a stimulus
Flagella
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Chemical stimulus
Chemotaxis
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Light stimulus
Phototaxis
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Flagellar arrangements
Monotrichous, lopotrichous, ampitrichous, peritrichous
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Single flagellum at one end
Monotrichous
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Two flagellum each protruding from either ends of the cell.
Ampitrichous
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Small bunches of flagella emerging from one end
Lopotrichous
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Flagella is dispersed over the surface of the cell.
Peritrichous
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Filament of flagella is made up of protein
Flagellin
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Rigid tubular structure made of pilin proteins.
Pili
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Found only in gram-negative cells
Pili
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Functions to join bacterial cells for partial DNA transfer called conjugation.
Pili
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Partial DNA transfer
Conjugation
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Basically facilitates genetic exchange.
Pili
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Fine, proteinaceous, hairlike bristles emerging from the cell surface.
Fimbriae
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Extends from the cell surfaces, enabling cells to stick to surfaces and form pellicles (liquid) and biofilms (solid).
Fimbriae
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Function in adhesion to other cells and surfaces
Fimbriae
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All bacterial capsules are composed of polysaccharide except
Bacillus anthracis (contain polypeptide D-glutamate)
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Formed by gram-positive rods, only found in Bacillus and Clostridium spp
Spore
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Spore is formed by
Gram-positive rods
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Spore can only be found among
Clostridium, Bacillus