MOST COMMONLY USED STAINING PROCEDURE

Stains basophilic tissue components

Placing a cover slip with a few drops of CANADA BALSAM as a mounting medium on processed tissue.

Acidic dyes

Removal of clearing agent. Its purpose is TO FILL THE TISSUE GAP with a solid medium (wax)

Glycogen, carbohydrate-rich molecules

Staining

Placing the tissue in paraffin blocks. Paraffin penetrates all intercellular spaces and even into the cells making the tissue MORE RESISTANT TO SECTIONING.

A method of preparation of tissue for microscopic examination

Histotechniques

CUTTING OF TISSUE by use of MICROTOME about 3-5 u in thickness. The cut tissue, paraffin ribbon are placed in a basin of water.

STRIATION OF MUSCLES, NUCLEI, ERYTHROCYTES

Basic Dyes

Differential staining of blood cells

The Greek root histo can be translated as either

Organization of the Body

ELASTIC STAIN

PREPARATION OF TISSUES FOR STUDY

Stains that differentiate acidic and basic components of the cell. Stains that differentiate fibrous component of the matrix. Metallic salts that precipitate tissue forming metal deposits on them.

Study of the tissues of the body and how these tissues are arranged to constitute organs. Not only tissues but also that of cells, organs and system.

Immersing the tissue in increasing concentration of alcohol to REMOVE WATER from the tissue.

Since alcohol is insoluble with paraffin, it must be replaced by a clearing agent so that I can be IMPREGNATED WITH PARAFFIN WAX.

Stains acidophilic tissue components

Label the organ on the prepared slides.

Involves all aspects of tissue biology, with the focus on how cells’ structure and arrangement optimize functions specific to each organ.

Prior grossing sample must be

RETICULAR FIBERS

Submerging the specimen in chemical substances in order to PRESERVE THE TISSUE.