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1
Other term for environmental technology
green technology, clean technology
2
It involves manipulation of molecules like RNA, DNA and PROTEINS
molecular biology
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encompasses a broad range of innovations and practices that aim to address environmental challenges and promote sustainability
environmental technology
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convert sunlight into electricity.
photovoltaic cells
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harness the kinetic energy of the wind to generate electricity.
wind turbines
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use flowing water to generate electricity.
dams and turbines
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like wood, crop residues, and waste are converted into energy through combustion or biofuel production.
organic materials
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refers to the entire collection of viruses that exist within a particular ecosystem or environment.
virosphere
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At the time of writing, only about _____% to _____% of microorganisms have been cultured.
0.1-1
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focuses on the study of biological processes at the molecular level.
molecular biology
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is used to amplify a specific DNA sequence. It is essential for DNA cloning, sequencing, and various molecular biology applications.
polymerase chain reaction
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It plays a crucial role in organizing, analyzing, and interpreting large volumes of biological information, such as DNA sequences, protein structures, and gene expression data.
bioinformatics
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are powerful tools used in molecular biology and genomics to simultaneously analyze the expression levels of thousands of genes or other nucleic acid sequences in a single experiment.
microarrays
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is the study of the genomes of whole communities of microscopic life forms.
metagenomics
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Scope: focuses on the genetic material found in entire communities of microorganisms
metagenomics
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Sample type: involves the analysis of DNA extracted from environmental samples
metagenomics
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Research Goal: to assess the genetic diversity, community structure, and functional potential of entire microbial communities in various environments.
metagenomics
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Scope: deals with the study of the genetic material (DNA) of individual organisms.
genetics
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Sample type: involves the analysis of DNA from a specific organism
genetics
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Research Goal: include understanding the inheritance of traits, identifying specific genes responsible for certain traits or diseases, and studying the molecular mechanisms underlying genetic processes in a single organism.
genetics
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is a microbiological technique used to selectively cultivate specific microorganisms from complex environmental samples.
culture enrichment
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It is used to to study the metabolic activities and identities of microorganisms within complex microbial communities.
stable isotope probing
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The first step in SIP involves introducing a specific isotopically labeled compound into the environment or experimental system.
isotopic labeling
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Microorganisms in the environment take up and metabolize the isotopically labeled compound if it is a source of nutrients or energy for them.
microbial uptake
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At various time points after the addition of the labeled compound, samples of the microbial community are collected.
sampling
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At various time points after the addition of the labeled compound, samples of the microbial community are collected. Adding _____
5-bromo-2-deoxyuridine
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During this process, the samples are layered on top of a heavy isotope solution and centrifuged. As the centrifuge spins, molecules with different densities will separate along the gradient.
density gradient centrifugation
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It is a technique used in immunology and molecular biology to specifically capture and isolate a target molecule, typically a protein, from a complex mixture.
immunocapture
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is a molecular biology technique used to identify and study the activity of specific microorganisms within complex microbial communities by tracking the incorporation of stable isotopes into their RNA molecules.
rna stable isotope probing
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It is a molecular biology technique used to selectively enrich and identify differentially expressed genes between two distinct RNA populations, typically representing a "tester" sample and a "driver" sample.
suppressive subtraction hybridization
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Contaminated sources
tester sample
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Non contaminated sources
driver sample
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SSH begins with the isolation of RNA (or DNA) from two different sources or conditions of interest.
sample preparation
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Complementary DNA (cDNA) is synthesized from the RNA in both the tester and driver populations.
cdna synthesis
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can be done using reverse transcription, and each cDNA population is labeled with different adaptors or linkers.
cdna synthesis
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cDNA synthesis can be done using ___________, and each cDNA population is labeled with different adaptors or linkers.
reverse transcription
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The tester cDNA is divided into two parts. One part is left unmodified, while the other part is subjected to a suppression step, where it is made less abundant by hybridizing it with an excess of driver cDNA.
hybridization