- Compact masses of dark, septate hyphae - Round to oval asci containing 2-8

Black Piedra Culture:

Microscopic Examination: - Cluster Budding yeast - Spaghetti and meatballs appearance

Culture ·Black, yeasty colonies · Develop short olive gray mycelia with age

Culture · No growth in routine fungal culture medium ·Yeast like colonies may observed only after the culture medium is overlaid with olive oil

Black Piedra, Causative agent:

Direct Examination: Tightly packed dichotomous branched hyphae

Wood's lamp - golden yellow fluorescence

Soft, white, yellowish, beige, or greenish nodules on the hair shaft

- Infection of the hair shaft that produces hair breakage - Due to poor personal hygiene - May disseminate in immunocompromised individuals

Microscopic examination ·Branched, septate hyphae ·2 celled oval yeast cells with melanized cell walls

Caused by a black colored yeast-like fungus

- Showed septate hyphae, arthroconidia and budding blastoconidia

Black Piedra uses what type of specimen?

Less firmly attached

Hard, dark brown to black gritty nodules on hair shaft

Characterized by scaly patches of hypo to hyper pigmentation of skin of moist humid areas

Dandruff in adults; Cradle cup in babies

- Fungal infection of the hair scalps

golden yellow fluorescence

Culture: SDA

Tinea Versicolor urease?

Fungal infection of facial, axillary, or genital hairs

Are cosmetic fungal infections of the skin or shaft

·Less commonly the scalp

Characterized by painless, black, non-scaly patches present on the palm and sole; more commonly in females.

- Raised, waxy appearance colonies - Radial furrow and irregular folds

- Cream-colored, moist, and smooth - SDA culture with olive oil overlay: Fried egg colonies appear

Causative agent: Piedraia hortae

Seborrheic dermatitis Atopic dermatitis Folliculitis Disseminated infection

Caused by a black colored yeast-like fungus - Hortaea wernecki

Tinea Nigra uses what type of specimen for laboratory diagnosis:

Wood's lamp - golden yellow fluorescence

Piedraia hortae

Causative agent: Malassezia furfur

White Piedra uses what type of microscopic examination?

Firmly attached to the hair shaft

Laboratory Diagnosis Microscopic examination uses for Tinea Versicolor?

A lipophilic fungus

Presence of asci: Negative

carbonaceous; can house an asci

The white Piedra also mounts?

Less commonly in beard and pubic areas

Direct Examination: Arthrospores and blastospore can be seen

Causative agent: Trichosporon spp.

Nodules: On the surface of the hair shaft, softer, easy to separate from hair

Treatment: Topical ketoconazole cream

Tinea Versicolor other term?

- Intertwined hyaline septate hyphae - Breaking up into oval or rectangular arthroconidia

Black Piedra uses what type of microscopic examination?

White Piedra is a urease?

Laboratory Diagnosis: Specimen used for Tinea Versicolor?

Trichosporon spp.

- No living tissue invaded - No cellular response from the host - No pathological changes elicited

Culture: SDA or PDA

Nodules: Over and around the hair shaft, hard, difficult to detach from the hair

Causative agents: Hortaea werneckii Exophiala werneckii Phaeoannellomyces werneckii

Presence of asci: Positive

Noninflammatory and Nonpruritic (rarely pruritic) Lesions

Areas rich in sebaceous glands are commonly involved