Costly

Contain indicator components that will distinguish one microorganism from another through biochemical reactions resulting to observable changes in the media. • e.g., MacConkey Agardyes: neutralred and crystal violet), Eosin Methylene Blue Agar (for E.coli and other enterics)

exact chemical composition

These are usually pure chemical substances.

For growing large number of bacteria

use of test tubes

Agar Melts at ___, solidifies at ___

Increase the number of desired microorganisms to detectable levels

Let the Nutrient Agar cool to around ___°C.

• allows growth of most organisms • e.g., Nutrient Agar, Nutrient Broth, Plate Count Agar

Colorless colonies

Red-colored colonies

use of petri dishes

Incline the nutrient agar tubes ät an angle of around 40 degrees from a flat surface.

Absence of oxygen

Solidifying agent with no nutritive value

____ vary from one type of media to another.

For research - for instance in studying the specific chemical requirements of certain microorganisms.

Large amounts of oxygen

For enumeration, isolation, and characterization

____ microorganisms can degrade agar.

Color change due to biochemical reaction

allows differentiation of microorganisms

basic elements in the cell for biosynthesis and energy production.

These are usyally digested crude products of various sources.

Small amount of oxygen

Lactose fermenters (Red-colored colonies) Nonlactise fermenters (Colorless colonies)

exact composition is not known; extracts and digests of yeasts, meat, or plants; undefined components.

test for metabolic activities • e.g., Citrate Agar (for microorganisms that can use citrate as carbon source)

Rich enough to allow growth of the majority.

Agar 0.5-0.7%

<5.5

For determination of bacterial motility

Incline the nutrient agar tubes at an angle of around 20 degrees from a f l a t surface.

Colorless colonies, no biochemical reaction.

These are usually with minimal composition necessary for the growth of the microorganisms.

Contain additional nutrients for the cultivation of fastidious.microorganisms. • e.g., Blood Agar Plate (BAP) and Chocolate Agar Plate,(CAP) #MAP + Bloo

Al purpose,nonselectivemedium. Wide variety of non-fastidious microbes.

>8

suppress growth of u n w a n t e d microorganisms and encourage growth of desired microorganisms with i n h i b i t o r s • e.g., Mannitol Salt Agar (for Staphylococcus)

Agar 1.5-2.0%

>5.5 and <8

Agar 0%

Blood hemolysis. Fastidious microbes.