basic elements in the cell for biosynthesis and energy production.

____ vary from one type of media to another.

<5.5

>5.5 and <8

>8

Solidifying agent with no nutritive value

____ microorganisms can degrade agar.

Agar Melts at ___, solidifies at ___

use of test tubes

use of petri dishes

exact chemical composition

These are usually with minimal composition necessary for the growth of the microorganisms.

Costly

These are usually pure chemical substances.

For research - for instance in studying the specific chemical requirements of certain microorganisms.

exact composition is not known; extracts and digests of yeasts, meat, or plants; undefined components.

These are usyally digested crude products of various sources.

Rich enough to allow growth of the majority.

Agar 1.5-2.0%

Agar 0%

Agar 0.5-0.7%

For enumeration, isolation, and characterization

For growing large number of bacteria

For determination of bacterial motility

Absence of oxygen

Large amounts of oxygen

Small amount of oxygen

• allows growth of most organisms • e.g., Nutrient Agar, Nutrient Broth, Plate Count Agar

Al purpose,nonselectivemedium. Wide variety of non-fastidious microbes.

suppress growth of u n w a n t e d microorganisms and encourage growth of desired microorganisms with i n h i b i t o r s • e.g., Mannitol Salt Agar (for Staphylococcus)

allows differentiation of microorganisms

Contain indicator components that will distinguish one microorganism from another through biochemical reactions resulting to observable changes in the media. • e.g., MacConkey Agardyes: neutralred and crystal violet), Eosin Methylene Blue Agar (for E.coli and other enterics)

Colorless colonies, no biochemical reaction.

Color change due to biochemical reaction

Lactose fermenters (Red-colored colonies) Nonlactise fermenters (Colorless colonies)

Red-colored colonies

Colorless colonies

Increase the number of desired microorganisms to detectable levels

Contain additional nutrients for the cultivation of fastidious.microorganisms. • e.g., Blood Agar Plate (BAP) and Chocolate Agar Plate,(CAP) #MAP + Bloo

Blood hemolysis. Fastidious microbes.

test for metabolic activities • e.g., Citrate Agar (for microorganisms that can use citrate as carbon source)

Incline the nutrient agar tubes at an angle of around 20 degrees from a f l a t surface.

Incline the nutrient agar tubes ät an angle of around 40 degrees from a flat surface.

Let the Nutrient Agar cool to around ___°C.