問題一覧
1
What is a light microscope?
Uses light and a system of lenses to magnify small objects
2
What is phase contrast microscopy?
A type of light microscope that enhances the contrast of transparent and colorless specimens.
3
What is DIC microscopy
A type of light microscope used to enhance the contrast of transparent and colorless specimens, but in 3D.
4
What is a fluorescence microscope?
A type of light microscope that uses specific light to make objects glow.
5
What is conventional fluorescence microscopy?
A type of light microscope that uses fluorescent dyes to see specific structures within a sample.
6
What is confocal fluorescence microscopy?
A type of light microscope that uses a laser to scan a sample, creating sharp 3D images
7
Difference between confocal
• Normal confocal has blue laser that excites column of sample • Two photo confocal has red laser that excites small spot of sample
8
What is a bright-field microscope?
A type of light microscope that uses light to view a sample, showing dark specimens against a bright background.
9
What is a light sheet microscope?
A type of microscope that takes quick, high-resolution pictures of a slice of a sample, reducing damage to the sample.
10
Difference between bright field, contrast, fluorescence microscope (respectively)
Showing dark specimen against a bright background, Enhances contrast, Uses specific light to make objects glow
11
What is an electron microscope?
Uses a beam of electrons to create highly magnified images of tiny objects.
12
What is histology
The study of tissues.
13
What is HE staining
Colors tissue samples to make cell parts easier to see.
14
What are gastric polyps?
Are small abnormal growths in the stomach
15
What is ISH
A technique to find specific DNA and RNA sequences within a tissue or cell.
16
What is FISH
Same as ISH but uses a fluorescently labeled probe
17
What is cell culture
Growing cells in labs
18
What is tissue engineering
Creation of tissues in labs
19
What is the main reason for using a microscope?
To see small objects that are invisible to the naked eye.
20
What is PCR
To make copies of DNA
21
How to isolate protein
By breaking cells, separating, and purifying the protein.
22
How to identify protein?
Use mass spec or antibody.