問題一覧
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methods and procedures used by Biologists to study macro and microorganisms
Biological Techniques
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Provides a magnified image of organisms or cells
Microscopy
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found inside the eye piece is where the final magnified image can be viewed, it usually has a magnification of 10x or 15x
Ocular Lens
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found attached to the nosepiece, vary in levels of magnification from x4 up to x1000 or more
Objective Lenses
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Is responsible for the regulation of light for brightness and contrast adjustments
Condenser & Diaphragm
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Are the fastest and simplest slides to prepare
Wet mounts
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Refers to the adhesion of the specimen to the slide and killing microorganisms to stop their movement
Fixation
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is the method of coloring certain cell structures by applying stains or dyes which are made of positive/negative ions
staining
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Dyes are composed of
chromophore and counter ion
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positive ion
Basic Dye
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Negative Ion
Acidic Dye
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If the dye directly interacts with the specimen
Positive Stain
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If it reacts with the environment or background it is classified as
Negative Stain
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All structures the same is used to emphasize to certain structures
Simple Stain
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distinguishes organisms or structures based on their interaction of different dyes
Differential Stain
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Makes use of light to fire sectioned specimen up to 200x magnification
Compound Microscope
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makes use of a stream of electrons to study the 3-D surface of a specimen
Scanning Electrons Microscope
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Makes use of a stream of electrons to study the internal structures of sectioned specimen
Transmission Electron Microscope
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Makes use angled light to view 3-D structures of specimen
Stereomicroscope
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A process of thinking used by scientist to collect information and provide scientific explanations
Scientific Method
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Reasoning that is based on specific observations which lead to general conclusions
Inductive Reasoning/ Bottom-up Approach
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Reasoning that is based on general principles to explain specific observations
Deductive Reasoning/Top-down approach
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A controlled observation to test a hypothesis, it consists of sample size, variables, controls, and statistical analysis
Experimental Designs
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Experiments where there is complete control over all extraneous variables to predict the effect of the manipulated independent variable
True Experimental Research Design
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Experiments wherein the independent variable is manipulated to see its effect on the dependent variable
Quasi-experimental Research Design
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Experiments wherein there is little to no control over extraneous variables
Pre-experimental Research Design
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States that life originates from non-living matter. originated from Aristotle and was believed until the 17th century
Theory of Abiogenesis aka Spontaneous Theory
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States that life originates from pre-existing life
Theory of Biogenesis
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stains negatively charged molecules
basic stains
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stains positively charged molecules
acidic stains
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stains the background, not the specimen
Negative Stains