Microscopy

45問 • 1年前

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is the technical field of using microscopes to view samples & objects that cannot be seen with the unaided eye (objects that are not within the resolution range of the normal eye).

Microscopy

an instrument that magnifies an image and allows visualization of greater detail than is possible with the unaided eye

Microscope

To visualize bacteria, fungi, parasites and even viruses.

Microbiology

to visualize the cells in the blood, BM, lymph, and other body fluids

Hematology

to visualize the tissue biopsies, cytological specimens and gynecological specimens

Histopathology and Cytology

Instrument design to make fine details visible

Microscope

Microscope 3 Tasks:

Magnification, Resolution, Contrast

Produce a magnified image of the specimen

Magnification

Separate the details in the image

Resolution

Render the details visible to the eye, camera, or other imaging device.

Contrast

measure of relative velocity at which light passes through.

Refractive Index

ability of lenses to separate closely distant objects

Resolving Power

the extent to which detail in the magnified objects are maintained

Resolution

make objects stand out from the background.

Contrast

to retain focus when objective lens is changed.

Parfocal

to maintain the focus at the center from the previous lens to another objective lens.

Parcentral

considered to be the first microscope.

Simple Microscope

Created the first microscope

Anton Van Leeuwenhoek

Types of Microscope

Bright field Microscope, Dark field Microscope, Phase Contrast Microscope, Fluorescent Microscope, Polarizing Microscope, Electron Microscope

DARK IMAGE AGAINST BRIGHT BACKGROUND.

Bright Field Microscope

BRIGHT IMAGE AGAINST DARK BACKGROUND

Dark Field Microscope

PRINCIPLE: Light source provides the light that aims into the specimen in stage. Stains absorb the light and this contrast allows the viewer to view the specimen.

Bright Field Microscope

FUNCTION:  For demonstration of organisms that are hard to visualize in bright microscope.  Example: bacteria that causes syphilis and Leptospirosis.

Dark Field Microscope

THREE CATEGORIES OF COMPONENTS OF MICROSCOPE

Magnifying Parts, Illuminating Parts, Mechanical Parts

is the object being examined.

Specimen

These are used to adjust the focus of the microscope.

Coarse Adjustment Knob, Fine Adjustment Knob

is used to move the stage up and down to bring the specimen into rough focus

Coarse Adjustment Knob

is used for finer focusing.

Fine Adjustment Knob

The lens the viewer looks through to see the specimen.  usually contains a 10X or 15X power lens.

Eyepiece

Useful as a means to change focus on one eyepiece so as to correct for any difference in vision between your two eyes

Diopter Adjustment

connects the eyepiece to the objective lenses

Body Tube (Head)

This is the part of the microscope that holds two or more objective lenses and can be rotated to easily change power (magnification)

Revolving Nosepiece

connects the body tube to the base of the microscope

Arm

Brings the specimen into general focus

Coarse Adjustment

Fine tunes the focus and increases the detail of the specimen.

Fine Adjustment

A rotating turret that houses the objective lenses. The viewer spins the nosepiece to select different objective lenses.

Nosepiece

The flat platform where the slide is placed.

Stage

Metal clips that hold the slide in place

Stage Clips

These knobs move the stage left and right or up and down.

Stage Control

The hole in the middle of the stage that allows light from the illuminator to reach the specimen

Aperture

This switch on the base of the microscope turns the illuminator off and on

On/Off Switch

The light source for a microscope.  Older microscopes used mirrors to reflect light from an external source up through the bottom of the stage; however, most microscopes now use a low-voltage bulb

Illumination

Adjusts the amount of light that reaches the specimen.

Iris Diaphragm

Gathers and focuses light from the illuminator onto the specimen being viewed.

Condenser

supports the microscope and it’s where illuminator is located.

Base

Introduction to Histology

Almira Coleen · 28問 · 1年前

Introduction to Histology