暗記メーカー
ログイン
Microbiology
  • ユーザ名非公開

  • 問題数 100 • 10/17/2023

    記憶度

    完璧

    15

    覚えた

    35

    うろ覚え

    0

    苦手

    0

    未解答

    0

    アカウント登録して、解答結果を保存しよう

    問題一覧

  • 1

    Which type of microbes live in your stomach?

    Acidophiles

  • 2

    Chloronates swimming pools is an example of _________ ?

    Halogens

  • 3

    What Method would you use to quantify cells that are sparse in a solution?

    Filtration

  • 4

    By ho much increase in atmosphericpressure (atm) if you were to dive 30 meters?

    3

  • 5

    Microbial growth refers o the size change of an individual microbe?

    False

  • 6

    The thermal death time for this culture under this condition is how many minutes?

    30 minutes

  • 7

    Compared to moist heat, dry heat requires ______ to sterilize?

    Longer time, higher temperature

  • 8

    From the list below, which is the most resistant to methods of deactvation/killing?

    prions

  • 9

    Which biosafety level is required when Dr. pham was doing HIV reserch?

    BSL-3

  • 10

    Blood Agar contains intact RBCs which are used to determine if a microbe has the ability to lyse blood cells.

    a differential medium & complex medium

  • 11

    In the Ames test, what is the purpose of positve selection?

    To enable microbes to grow on agar with Antibiotics

  • 12

    The standard (control) used to compare and determine chemical efficacy is:

    Phenol

  • 13

    The theraputic drug index refers to:

    The concentration or dosing that can be tolerated

  • 14

    To minimize the development of resistance, it is advised to:

    use a combination of drugs

  • 15

    Substitution (point mutation) involves:

    Exchanging one base for another

  • 16

    What is the goal of developing a safe bacterial control?

    Kill most bacteria without causing harm to humans

  • 17

    Which enzyme can proofread DNA during replication?

    DNA Polymerase III

  • 18

    For disinfectant #3 the MIC is:

    0.5 ug/mL

  • 19

    Which mutagen results iis pyrimidine dimers?

    UV Radiation

  • 20

    All food and products must pass FDA regulation before selling to consumers to ensure:

    They are safe for consumption

  • 21

    Where does most gene regulation occur in prokaryotes?

    Transcriptional Level

  • 22

    List the steps in order:

    Lag phase, Exponential Phase, Stationary Phase, Death Phase

  • 23

    Why is it said that nucleic acid polymerization occurs 5’ to 3’?

    The #3 carbon is where the chain grows and #5 carbon is in “front”

  • 24

    The trp operon is transcribed when tryptophan is _______? The lac oeron is transcribed when lactose is ________ ?

    Absent, Present

  • 25

    If a researcher sets up a PCR reaction, but mistakenly uses a heat-sensitive polymerase, not a Taq polymerase, what will happen?

    The PCR will stop after one cycle

  • 26

    Which condition ensures maximal trascription of the lac operon?

    Abundant Lactose

  • 27

    Why would recombinant DNA molecules be inserted into a host cell?

    It can be copied, transcribed, and translated into the desired protein

  • 28

    Both prokaryotic and eukaryotic cells have their transcription and translation happening simultaneously in the cytoplasm

    True

  • 29

    Disinfection can involve both chemical and physical methods

    True

  • 30

    Filtration is a method to concentrate the sparse number of microbes so that we are able to count them

    True

  • 31

    What accidental discovery led alexander fleming’s penicillin breakthrough?

    Contamination in a petri dish

  • 32

    The energy required for DNA replication comes from the:

    Triphosphate deoxyribonucleotide

  • 33

    DNA replication requires DNA primers in order to initiate

    False

  • 34

    Transcription starts at a region of DNA called the

    Promoter

  • 35

    Why do we use heat instead of helicase, topoisomerase, etc in PCR?

    Too many enzymes meas too many factors we must accomodate fr

  • 36

    Missense Nonsense Silent

    Could encode a different amino acid Could change an amino acid into a stop codon Could change a codon into one that encodes the same amino acid

  • 37

    If a medium changes color after incubation with specific types of microorganisms it is a:

    Differential Medium

  • 38

    Which of the following categories do the vast majority of pathogens fall into?

    Mesophiles

  • 39

    Which type of mutation results if part of a chromosomebreaks off and reattaches to a different chromosome?

    Gross

  • 40

    Negative selection

    To disable microbes from growing on agar lacking tryptophan

  • 41

    Positive Selection

    To enable microbes to grow on agar with antibiotics

  • 42

    Ames test

    To enable microbes to grow on agar lacking histidine

  • 43

    Chromosomal and plasmid DNA replication bubbles begin

    At the origin of replication

  • 44

    Origins of replication may be numerus on a chromosomes

    true

  • 45

    Why is Taq polymerase unique?

    Survive extreme low and high temperature

  • 46

    The regulation control in prokaryotic that represents a key to a car is:

    Positive Control

  • 47

    Researchers working on the treponeme microbes that cause syphilis MUST isolate large numbers of the bacteria from an infected rabbit. Why would this be true?

    The bacterium cannot be cultured in a laboratory medium

  • 48

    Which of the following is a low-level disinfectant?

    soap

  • 49

    From the list below, which is not a target of antimicrobial activity?

    Increased cell-pumping activity

  • 50

    Which condition would prevent the trp operon from being transcribed?

    High levels of tryptophan

  • 51

    During the elongation phase of translation, an mRNA codon will pass through which order of the tRNA bringing sites on the ribosome?

    A site, P site, E site

  • 52

    Name one person (last name only) who got the nobe prize for the discovery of DNA structure

    Watson

  • 53

    Bioinformatic:

    Analyze and find the info of a gene sequence

  • 54

    Polymerase Chain Reacton:

    Make copies of the gene sequence

  • 55

    Agarose gel electrophoresis

    Verify the size of a gene sequence

  • 56

    qPCR or sequencing

    Verify the codes of the gene sequence

  • 57

    When using restricton enzymes for gene insertion during molecular cloning, what ritical issue arrises if the orientation of the gene of interest is not correct?

    The gene will be inserted in reverse orientation, affectting gene expression

  • 58

    Which mutagen results in pyrimidine dimers?

    UV Radiation

  • 59

    select all that can affecct bacterial growth and enzyme activities.

    osmolarity, water, temperature, atmospheric pressure, pH

  • 60

    The use of lysosome during the preparation of cheese and wines is effective in reducing the number of

    bacteria

  • 61

    What is the primary function of base-excision repai ad mismatch repair mechanism?

    replace incorrect bases

  • 62

    When an insertion or detection of one or two nucleotides occurs in a coding region for a protein this type of mutation occurs

    Frameshift

  • 63

    Where specifically in a eukaryotic cell does the process of translation occur

    Rough ER

  • 64

    In a laboratory exploring DNA structure and it’s aplications, scientists are studying the factors influencing the separation of the DNA strands. During experiments involving DNA strand for processes like repliation or analysis, cytosine (C) and guanine (G) are consistantly identified at key positions to their:

    Formation of two hydrogn bonds, easing the separation of DNA strands during the laboratory process

  • 65

    Transfection:

    Inserting DNA into Eukaryotic cells

  • 66

    Transformation:

    Inserting DNA into bacteria

  • 67

    Transduction:

    Inserting Virus into bacteria

  • 68

    Organize the steps of PCR

    95 C to separate, 50 C to anneal, 72 C to extend

  • 69

    What are some examples of mechanisms of resistance?

    Utilize different metabolic pathways, Produce enzyme to breakdown drugs, Synthesize biofilm, Block drug entry

  • 70

    In Griffith's experiment on bacterial transformation, what crucial discovery indicated that a bacterial plasmid could turn harmless bacteria into deadly strains?

    The transformation of non-pathogenic bacteria into pathogenic ones in the presence of a heat-killed pathogenic strain

  • 71

    Before complementary base pairing can occur in DNA replication:

    The DNA molecule must be unwould and separate

  • 72

    The nucletide sequence AUGGUC

    Could be part of an mRNA

  • 73

    The primary reason environmental conditions play a role in microbial growth is because these conditons affect____

    Activity and folding of enzymes and proteins

  • 74

    In griffith’s experiment, what was determined to be the key factor responsible for the transformation of non-pathogenic bacteria into pathogenic ones when exposed to a heat-killed pathogenic strain?

    The exchange of plasmids between the bacteria

  • 75

    The RNA polymerase will constituitively bind and epress the regulatory gene?

    True

  • 76

    LAC Operons are inducible where the addition of lactose is required to activate gene expression

    Inducible

  • 77

    This gah is illustrating a:

    Decimal reduction time

  • 78

    Select all the desired quorum sensing:

    Cells sensing the concentration of their population, Cells will not fluoresce until there is enough of them, Bacteria will continue to grow until there is enough of them to begin an invasion

  • 79

    What is the mechanism where opportunistic bacteria communicate with each other prior to an all-out invasion?

    Quorum sensing

  • 80

    A broad spectrum antimicrobial can be prescribed to treat all infections

    false

  • 81

    Which of the following is the preffered method of determining the efficacy of an antimicrobial chemical in the European union?

    Kelsey-sykes capacity test

  • 82

    Okazaki fragments occur because

    Polymerization 5’ to 3’ occurs facing opposite space created by helicase on the template

  • 83

    The RNA type which brings amino acids to a ribosome is

    tRNA

  • 84

    Why is the DNA synthesis of the lagging strand considered discontinuous?

    Synthesis is moving in the opposite direction from te replication fork

  • 85

    Which of the following would not be used during DNA fingering (gene cloning) procedure?

    Fluorescent Nucleotides

  • 86

    Which of the following mutations would most likely to have a harmful effect on an organism?

    A single nucleotide insertion downtstream of the start gene sequence

  • 87

    Which enzyme places the RNA nucleotides which are complementary to DNA nucleotides down to begging DNA replication?

    Primase

  • 88

    It is our protein that expresses the traits to be passed down from generation to generation

    False

  • 89

    In the context of prokaryotic cells, how can restriction enzymes be analogously compared to a biological immune system response against bacteriophages?\

    Restriction enzymes recognize and destroy specific DNA sequences, similar to how theimmune system targets specific pathogens

  • 90

    Which of the following is considered a mechanism of resistance for the protein synthesis?

    Produce natural inhibition against the grus from binding

  • 91

    Why are the endospores vials used to test autoclave function?

    They are very resistant life forms

  • 92

    Which type of organism would be expected to grow at 60 C

    Thermophile

  • 93

    The Ames test determines if chemical is a ccarcinogen (causes cancer)

    False

  • 94

    Which of the following is/are involved in translation. Choose all that apply

    tRNA, Amino Acids, mRNA, rRNA

  • 95

    In order for DNA to be copied, this must first be attached to the template strands at an origin of replication:

    Primer

  • 96

    The trm cDNA refers to

    DNA tha is complementary to template RNA

  • 97

    What type of mutation is the following?

    missense

  • 98

    Where does most gene-regulation occur in prokaryotes?

    Transcriptional level

  • 99

    A medium that contains high salt to inhibit some type of organism is considered?

    Selective

  • 100

    Beta-lactamase is an enzyme that bacteria resistant to penicillin produce?

    True