問題一覧
1
Which type of microbes live in your stomach?
Acidophiles
2
Chloronates swimming pools is an example of _________ ?
Halogens
3
What Method would you use to quantify cells that are sparse in a solution?
Filtration
4
By ho much increase in atmosphericpressure (atm) if you were to dive 30 meters?
3
5
Microbial growth refers o the size change of an individual microbe?
False
6
The thermal death time for this culture under this condition is how many minutes?
30 minutes
7
Compared to moist heat, dry heat requires ______ to sterilize?
Longer time, higher temperature
8
From the list below, which is the most resistant to methods of deactvation/killing?
prions
9
Which biosafety level is required when Dr. pham was doing HIV reserch?
BSL-3
10
Blood Agar contains intact RBCs which are used to determine if a microbe has the ability to lyse blood cells.
a differential medium & complex medium
11
In the Ames test, what is the purpose of positve selection?
To enable microbes to grow on agar with Antibiotics
12
The standard (control) used to compare and determine chemical efficacy is:
Phenol
13
The theraputic drug index refers to:
The concentration or dosing that can be tolerated
14
To minimize the development of resistance, it is advised to:
use a combination of drugs
15
Substitution (point mutation) involves:
Exchanging one base for another
16
What is the goal of developing a safe bacterial control?
Kill most bacteria without causing harm to humans
17
Which enzyme can proofread DNA during replication?
DNA Polymerase III
18
For disinfectant #3 the MIC is:
0.5 ug/mL
19
Which mutagen results iis pyrimidine dimers?
UV Radiation
20
All food and products must pass FDA regulation before selling to consumers to ensure:
They are safe for consumption
21
Where does most gene regulation occur in prokaryotes?
Transcriptional Level
22
List the steps in order:
Lag phase, Exponential Phase, Stationary Phase, Death Phase
23
Why is it said that nucleic acid polymerization occurs 5’ to 3’?
The #3 carbon is where the chain grows and #5 carbon is in “front”
24
The trp operon is transcribed when tryptophan is _______? The lac oeron is transcribed when lactose is ________ ?
Absent, Present
25
If a researcher sets up a PCR reaction, but mistakenly uses a heat-sensitive polymerase, not a Taq polymerase, what will happen?
The PCR will stop after one cycle
26
Which condition ensures maximal trascription of the lac operon?
Abundant Lactose
27
Why would recombinant DNA molecules be inserted into a host cell?
It can be copied, transcribed, and translated into the desired protein
28
Both prokaryotic and eukaryotic cells have their transcription and translation happening simultaneously in the cytoplasm
True
29
Disinfection can involve both chemical and physical methods
True
30
Filtration is a method to concentrate the sparse number of microbes so that we are able to count them
True
31
What accidental discovery led alexander fleming’s penicillin breakthrough?
Contamination in a petri dish
32
The energy required for DNA replication comes from the:
Triphosphate deoxyribonucleotide
33
DNA replication requires DNA primers in order to initiate
False
34
Transcription starts at a region of DNA called the
Promoter
35
Why do we use heat instead of helicase, topoisomerase, etc in PCR?
Too many enzymes meas too many factors we must accomodate fr
36
Missense Nonsense Silent
Could encode a different amino acid Could change an amino acid into a stop codon Could change a codon into one that encodes the same amino acid
37
If a medium changes color after incubation with specific types of microorganisms it is a:
Differential Medium
38
Which of the following categories do the vast majority of pathogens fall into?
Mesophiles
39
Which type of mutation results if part of a chromosomebreaks off and reattaches to a different chromosome?
Gross
40
Negative selection
To disable microbes from growing on agar lacking tryptophan
41
Positive Selection
To enable microbes to grow on agar with antibiotics
42
Ames test
To enable microbes to grow on agar lacking histidine
43
Chromosomal and plasmid DNA replication bubbles begin
At the origin of replication
44
Origins of replication may be numerus on a chromosomes
true
45
Why is Taq polymerase unique?
Survive extreme low and high temperature
46
The regulation control in prokaryotic that represents a key to a car is:
Positive Control
47
Researchers working on the treponeme microbes that cause syphilis MUST isolate large numbers of the bacteria from an infected rabbit. Why would this be true?
The bacterium cannot be cultured in a laboratory medium
48
Which of the following is a low-level disinfectant?
soap
49
From the list below, which is not a target of antimicrobial activity?
Increased cell-pumping activity
50
Which condition would prevent the trp operon from being transcribed?
High levels of tryptophan
51
During the elongation phase of translation, an mRNA codon will pass through which order of the tRNA bringing sites on the ribosome?
A site, P site, E site
52
Name one person (last name only) who got the nobe prize for the discovery of DNA structure
Watson
53
Bioinformatic:
Analyze and find the info of a gene sequence
54
Polymerase Chain Reacton:
Make copies of the gene sequence
55
Agarose gel electrophoresis
Verify the size of a gene sequence
56
qPCR or sequencing
Verify the codes of the gene sequence
57
When using restricton enzymes for gene insertion during molecular cloning, what ritical issue arrises if the orientation of the gene of interest is not correct?
The gene will be inserted in reverse orientation, affectting gene expression
58
Which mutagen results in pyrimidine dimers?
UV Radiation
59
select all that can affecct bacterial growth and enzyme activities.
osmolarity, water, temperature, atmospheric pressure, pH
60
The use of lysosome during the preparation of cheese and wines is effective in reducing the number of
bacteria
61
What is the primary function of base-excision repai ad mismatch repair mechanism?
replace incorrect bases
62
When an insertion or detection of one or two nucleotides occurs in a coding region for a protein this type of mutation occurs
Frameshift
63
Where specifically in a eukaryotic cell does the process of translation occur
Rough ER
64
In a laboratory exploring DNA structure and it’s aplications, scientists are studying the factors influencing the separation of the DNA strands. During experiments involving DNA strand for processes like repliation or analysis, cytosine (C) and guanine (G) are consistantly identified at key positions to their:
Formation of two hydrogn bonds, easing the separation of DNA strands during the laboratory process
65
Transfection:
Inserting DNA into Eukaryotic cells
66
Transformation:
Inserting DNA into bacteria
67
Transduction:
Inserting Virus into bacteria
68
Organize the steps of PCR
95 C to separate, 50 C to anneal, 72 C to extend
69
What are some examples of mechanisms of resistance?
Utilize different metabolic pathways, Produce enzyme to breakdown drugs, Synthesize biofilm, Block drug entry
70
In Griffith's experiment on bacterial transformation, what crucial discovery indicated that a bacterial plasmid could turn harmless bacteria into deadly strains?
The transformation of non-pathogenic bacteria into pathogenic ones in the presence of a heat-killed pathogenic strain
71
Before complementary base pairing can occur in DNA replication:
The DNA molecule must be unwould and separate
72
The nucletide sequence AUGGUC
Could be part of an mRNA
73
The primary reason environmental conditions play a role in microbial growth is because these conditons affect____
Activity and folding of enzymes and proteins
74
In griffith’s experiment, what was determined to be the key factor responsible for the transformation of non-pathogenic bacteria into pathogenic ones when exposed to a heat-killed pathogenic strain?
The exchange of plasmids between the bacteria
75
The RNA polymerase will constituitively bind and epress the regulatory gene?
True
76
LAC Operons are inducible where the addition of lactose is required to activate gene expression
Inducible
77
This gah is illustrating a:
Decimal reduction time
78
Select all the desired quorum sensing:
Cells sensing the concentration of their population, Cells will not fluoresce until there is enough of them, Bacteria will continue to grow until there is enough of them to begin an invasion
79
What is the mechanism where opportunistic bacteria communicate with each other prior to an all-out invasion?
Quorum sensing
80
A broad spectrum antimicrobial can be prescribed to treat all infections
false
81
Which of the following is the preffered method of determining the efficacy of an antimicrobial chemical in the European union?
Kelsey-sykes capacity test
82
Okazaki fragments occur because
Polymerization 5’ to 3’ occurs facing opposite space created by helicase on the template
83
The RNA type which brings amino acids to a ribosome is
tRNA
84
Why is the DNA synthesis of the lagging strand considered discontinuous?
Synthesis is moving in the opposite direction from te replication fork
85
Which of the following would not be used during DNA fingering (gene cloning) procedure?
Fluorescent Nucleotides
86
Which of the following mutations would most likely to have a harmful effect on an organism?
A single nucleotide insertion downtstream of the start gene sequence
87
Which enzyme places the RNA nucleotides which are complementary to DNA nucleotides down to begging DNA replication?
Primase
88
It is our protein that expresses the traits to be passed down from generation to generation
False
89
In the context of prokaryotic cells, how can restriction enzymes be analogously compared to a biological immune system response against bacteriophages?\
Restriction enzymes recognize and destroy specific DNA sequences, similar to how theimmune system targets specific pathogens
90
Which of the following is considered a mechanism of resistance for the protein synthesis?
Produce natural inhibition against the grus from binding
91
Why are the endospores vials used to test autoclave function?
They are very resistant life forms
92
Which type of organism would be expected to grow at 60 C
Thermophile
93
The Ames test determines if chemical is a ccarcinogen (causes cancer)
False
94
Which of the following is/are involved in translation. Choose all that apply
tRNA, Amino Acids, mRNA, rRNA
95
In order for DNA to be copied, this must first be attached to the template strands at an origin of replication:
Primer
96
The trm cDNA refers to
DNA tha is complementary to template RNA
97
What type of mutation is the following?
missense
98
Where does most gene-regulation occur in prokaryotes?
Transcriptional level
99
A medium that contains high salt to inhibit some type of organism is considered?
Selective
100
Beta-lactamase is an enzyme that bacteria resistant to penicillin produce?
True