問題一覧
1
Beta-lactamase is an enzyme that bacteria resistant to penicillin produce?
True
2
Which of the following mutations would most likely to have a harmful effect on an organism?
A single nucleotide insertion downtstream of the start gene sequence
3
Which biosafety level is required when Dr. pham was doing HIV reserch?
BSL-3
4
Transfection:
Inserting DNA into Eukaryotic cells
5
Before complementary base pairing can occur in DNA replication:
The DNA molecule must be unwould and separate
6
This gah is illustrating a:
Decimal reduction time
7
LAC Operons are inducible where the addition of lactose is required to activate gene expression
Inducible
8
Transduction:
Inserting Virus into bacteria
9
If a medium changes color after incubation with specific types of microorganisms it is a:
Differential Medium
10
Which condition ensures maximal trascription of the lac operon?
Abundant Lactose
11
Disinfection can involve both chemical and physical methods
True
12
Okazaki fragments occur because
Polymerization 5’ to 3’ occurs facing opposite space created by helicase on the template
13
Researchers working on the treponeme microbes that cause syphilis MUST isolate large numbers of the bacteria from an infected rabbit. Why would this be true?
The bacterium cannot be cultured in a laboratory medium
14
What type of mutation is the following?
missense
15
Filtration is a method to concentrate the sparse number of microbes so that we are able to count them
True
16
Which of the following is the preffered method of determining the efficacy of an antimicrobial chemical in the European union?
Kelsey-sykes capacity test
17
Microbial growth refers o the size change of an individual microbe?
False
18
The standard (control) used to compare and determine chemical efficacy is:
Phenol
19
In griffith’s experiment, what was determined to be the key factor responsible for the transformation of non-pathogenic bacteria into pathogenic ones when exposed to a heat-killed pathogenic strain?
The exchange of plasmids between the bacteria
20
Ames test
To enable microbes to grow on agar lacking histidine
21
Transformation:
Inserting DNA into bacteria
22
Which type of organism would be expected to grow at 60 C
Thermophile
23
Bioinformatic:
Analyze and find the info of a gene sequence
24
The nucletide sequence AUGGUC
Could be part of an mRNA
25
Missense Nonsense Silent
Could encode a different amino acid Could change an amino acid into a stop codon Could change a codon into one that encodes the same amino acid
26
Compared to moist heat, dry heat requires ______ to sterilize?
Longer time, higher temperature
27
The regulation control in prokaryotic that represents a key to a car is:
Positive Control
28
Blood Agar contains intact RBCs which are used to determine if a microbe has the ability to lyse blood cells.
a differential medium & complex medium
29
DNA replication requires DNA primers in order to initiate
False
30
The trp operon is transcribed when tryptophan is _______? The lac oeron is transcribed when lactose is ________ ?
Absent, Present
31
Origins of replication may be numerus on a chromosomes
true
32
During the elongation phase of translation, an mRNA codon will pass through which order of the tRNA bringing sites on the ribosome?
A site, P site, E site
33
select all that can affecct bacterial growth and enzyme activities.
osmolarity, water, temperature, atmospheric pressure, pH
34
From the list below, which is not a target of antimicrobial activity?
Increased cell-pumping activity
35
Polymerase Chain Reacton:
Make copies of the gene sequence
36
Negative selection
To disable microbes from growing on agar lacking tryptophan
37
Chloronates swimming pools is an example of _________ ?
Halogens
38
Name one person (last name only) who got the nobe prize for the discovery of DNA structure
Watson
39
The Ames test determines if chemical is a ccarcinogen (causes cancer)
False
40
When using restricton enzymes for gene insertion during molecular cloning, what ritical issue arrises if the orientation of the gene of interest is not correct?
The gene will be inserted in reverse orientation, affectting gene expression
41
Which type of microbes live in your stomach?
Acidophiles
42
It is our protein that expresses the traits to be passed down from generation to generation
False
43
Agarose gel electrophoresis
Verify the size of a gene sequence
44
Why are the endospores vials used to test autoclave function?
They are very resistant life forms
45
Which of the following is a low-level disinfectant?
soap
46
Which mutagen results iis pyrimidine dimers?
UV Radiation
47
Substitution (point mutation) involves:
Exchanging one base for another
48
Why would recombinant DNA molecules be inserted into a host cell?
It can be copied, transcribed, and translated into the desired protein
49
Which enzyme places the RNA nucleotides which are complementary to DNA nucleotides down to begging DNA replication?
Primase
50
A broad spectrum antimicrobial can be prescribed to treat all infections
false
51
Positive Selection
To enable microbes to grow on agar with antibiotics
52
Which mutagen results in pyrimidine dimers?
UV Radiation
53
Which of the following is considered a mechanism of resistance for the protein synthesis?
Produce natural inhibition against the grus from binding
54
Select all the desired quorum sensing:
Cells sensing the concentration of their population, Cells will not fluoresce until there is enough of them, Bacteria will continue to grow until there is enough of them to begin an invasion
55
In a laboratory exploring DNA structure and it’s aplications, scientists are studying the factors influencing the separation of the DNA strands. During experiments involving DNA strand for processes like repliation or analysis, cytosine (C) and guanine (G) are consistantly identified at key positions to their:
Formation of two hydrogn bonds, easing the separation of DNA strands during the laboratory process
56
The use of lysosome during the preparation of cheese and wines is effective in reducing the number of
bacteria
57
What is the goal of developing a safe bacterial control?
Kill most bacteria without causing harm to humans
58
Which type of mutation results if part of a chromosomebreaks off and reattaches to a different chromosome?
Gross
59
Why do we use heat instead of helicase, topoisomerase, etc in PCR?
Too many enzymes meas too many factors we must accomodate fr
60
Why is it said that nucleic acid polymerization occurs 5’ to 3’?
The #3 carbon is where the chain grows and #5 carbon is in “front”
61
In the Ames test, what is the purpose of positve selection?
To enable microbes to grow on agar with Antibiotics
62
Both prokaryotic and eukaryotic cells have their transcription and translation happening simultaneously in the cytoplasm
True
63
Where does most gene-regulation occur in prokaryotes?
Transcriptional level
64
Organize the steps of PCR
95 C to separate, 50 C to anneal, 72 C to extend
65
A medium that contains high salt to inhibit some type of organism is considered?
Selective
66
Which of the following categories do the vast majority of pathogens fall into?
Mesophiles
67
In the context of prokaryotic cells, how can restriction enzymes be analogously compared to a biological immune system response against bacteriophages?\
Restriction enzymes recognize and destroy specific DNA sequences, similar to how theimmune system targets specific pathogens
68
By ho much increase in atmosphericpressure (atm) if you were to dive 30 meters?
3
69
The primary reason environmental conditions play a role in microbial growth is because these conditons affect____
Activity and folding of enzymes and proteins
70
What Method would you use to quantify cells that are sparse in a solution?
Filtration
71
Which of the following is/are involved in translation. Choose all that apply
tRNA, Amino Acids, mRNA, rRNA
72
Which of the following would not be used during DNA fingering (gene cloning) procedure?
Fluorescent Nucleotides
73
Where does most gene regulation occur in prokaryotes?
Transcriptional Level
74
List the steps in order:
Lag phase, Exponential Phase, Stationary Phase, Death Phase
75
The thermal death time for this culture under this condition is how many minutes?
30 minutes
76
In Griffith's experiment on bacterial transformation, what crucial discovery indicated that a bacterial plasmid could turn harmless bacteria into deadly strains?
The transformation of non-pathogenic bacteria into pathogenic ones in the presence of a heat-killed pathogenic strain
77
For disinfectant #3 the MIC is:
0.5 ug/mL
78
When an insertion or detection of one or two nucleotides occurs in a coding region for a protein this type of mutation occurs
Frameshift
79
In order for DNA to be copied, this must first be attached to the template strands at an origin of replication:
Primer
80
Why is Taq polymerase unique?
Survive extreme low and high temperature
81
The RNA type which brings amino acids to a ribosome is
tRNA
82
What accidental discovery led alexander fleming’s penicillin breakthrough?
Contamination in a petri dish
83
The theraputic drug index refers to:
The concentration or dosing that can be tolerated
84
qPCR or sequencing
Verify the codes of the gene sequence
85
What is the mechanism where opportunistic bacteria communicate with each other prior to an all-out invasion?
Quorum sensing
86
The energy required for DNA replication comes from the:
Triphosphate deoxyribonucleotide
87
Where specifically in a eukaryotic cell does the process of translation occur
Rough ER
88
Chromosomal and plasmid DNA replication bubbles begin
At the origin of replication
89
Which enzyme can proofread DNA during replication?
DNA Polymerase III
90
What is the primary function of base-excision repai ad mismatch repair mechanism?
replace incorrect bases
91
What are some examples of mechanisms of resistance?
Utilize different metabolic pathways, Produce enzyme to breakdown drugs, Synthesize biofilm, Block drug entry
92
To minimize the development of resistance, it is advised to:
use a combination of drugs
93
Transcription starts at a region of DNA called the
Promoter
94
The RNA polymerase will constituitively bind and epress the regulatory gene?
True
95
All food and products must pass FDA regulation before selling to consumers to ensure:
They are safe for consumption
96
Which condition would prevent the trp operon from being transcribed?
High levels of tryptophan
97
Why is the DNA synthesis of the lagging strand considered discontinuous?
Synthesis is moving in the opposite direction from te replication fork
98
The trm cDNA refers to
DNA tha is complementary to template RNA
99
If a researcher sets up a PCR reaction, but mistakenly uses a heat-sensitive polymerase, not a Taq polymerase, what will happen?
The PCR will stop after one cycle
100
From the list below, which is the most resistant to methods of deactvation/killing?
prions