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Microbiology
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  • 問題数 100 • 10/17/2023

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    問題一覧

  • 1

    Beta-lactamase is an enzyme that bacteria resistant to penicillin produce?

    True

  • 2

    Which of the following mutations would most likely to have a harmful effect on an organism?

    A single nucleotide insertion downtstream of the start gene sequence

  • 3

    Which biosafety level is required when Dr. pham was doing HIV reserch?

    BSL-3

  • 4

    Transfection:

    Inserting DNA into Eukaryotic cells

  • 5

    Before complementary base pairing can occur in DNA replication:

    The DNA molecule must be unwould and separate

  • 6

    This gah is illustrating a:

    Decimal reduction time

  • 7

    LAC Operons are inducible where the addition of lactose is required to activate gene expression

    Inducible

  • 8

    Transduction:

    Inserting Virus into bacteria

  • 9

    If a medium changes color after incubation with specific types of microorganisms it is a:

    Differential Medium

  • 10

    Which condition ensures maximal trascription of the lac operon?

    Abundant Lactose

  • 11

    Disinfection can involve both chemical and physical methods

    True

  • 12

    Okazaki fragments occur because

    Polymerization 5’ to 3’ occurs facing opposite space created by helicase on the template

  • 13

    Researchers working on the treponeme microbes that cause syphilis MUST isolate large numbers of the bacteria from an infected rabbit. Why would this be true?

    The bacterium cannot be cultured in a laboratory medium

  • 14

    What type of mutation is the following?

    missense

  • 15

    Filtration is a method to concentrate the sparse number of microbes so that we are able to count them

    True

  • 16

    Which of the following is the preffered method of determining the efficacy of an antimicrobial chemical in the European union?

    Kelsey-sykes capacity test

  • 17

    Microbial growth refers o the size change of an individual microbe?

    False

  • 18

    The standard (control) used to compare and determine chemical efficacy is:

    Phenol

  • 19

    In griffith’s experiment, what was determined to be the key factor responsible for the transformation of non-pathogenic bacteria into pathogenic ones when exposed to a heat-killed pathogenic strain?

    The exchange of plasmids between the bacteria

  • 20

    Ames test

    To enable microbes to grow on agar lacking histidine

  • 21

    Transformation:

    Inserting DNA into bacteria

  • 22

    Which type of organism would be expected to grow at 60 C

    Thermophile

  • 23

    Bioinformatic:

    Analyze and find the info of a gene sequence

  • 24

    The nucletide sequence AUGGUC

    Could be part of an mRNA

  • 25

    Missense Nonsense Silent

    Could encode a different amino acid Could change an amino acid into a stop codon Could change a codon into one that encodes the same amino acid

  • 26

    Compared to moist heat, dry heat requires ______ to sterilize?

    Longer time, higher temperature

  • 27

    The regulation control in prokaryotic that represents a key to a car is:

    Positive Control

  • 28

    Blood Agar contains intact RBCs which are used to determine if a microbe has the ability to lyse blood cells.

    a differential medium & complex medium

  • 29

    DNA replication requires DNA primers in order to initiate

    False

  • 30

    The trp operon is transcribed when tryptophan is _______? The lac oeron is transcribed when lactose is ________ ?

    Absent, Present

  • 31

    Origins of replication may be numerus on a chromosomes

    true

  • 32

    During the elongation phase of translation, an mRNA codon will pass through which order of the tRNA bringing sites on the ribosome?

    A site, P site, E site

  • 33

    select all that can affecct bacterial growth and enzyme activities.

    osmolarity, water, temperature, atmospheric pressure, pH

  • 34

    From the list below, which is not a target of antimicrobial activity?

    Increased cell-pumping activity

  • 35

    Polymerase Chain Reacton:

    Make copies of the gene sequence

  • 36

    Negative selection

    To disable microbes from growing on agar lacking tryptophan

  • 37

    Chloronates swimming pools is an example of _________ ?

    Halogens

  • 38

    Name one person (last name only) who got the nobe prize for the discovery of DNA structure

    Watson

  • 39

    The Ames test determines if chemical is a ccarcinogen (causes cancer)

    False

  • 40

    When using restricton enzymes for gene insertion during molecular cloning, what ritical issue arrises if the orientation of the gene of interest is not correct?

    The gene will be inserted in reverse orientation, affectting gene expression

  • 41

    Which type of microbes live in your stomach?

    Acidophiles

  • 42

    It is our protein that expresses the traits to be passed down from generation to generation

    False

  • 43

    Agarose gel electrophoresis

    Verify the size of a gene sequence

  • 44

    Why are the endospores vials used to test autoclave function?

    They are very resistant life forms

  • 45

    Which of the following is a low-level disinfectant?

    soap

  • 46

    Which mutagen results iis pyrimidine dimers?

    UV Radiation

  • 47

    Substitution (point mutation) involves:

    Exchanging one base for another

  • 48

    Why would recombinant DNA molecules be inserted into a host cell?

    It can be copied, transcribed, and translated into the desired protein

  • 49

    Which enzyme places the RNA nucleotides which are complementary to DNA nucleotides down to begging DNA replication?

    Primase

  • 50

    A broad spectrum antimicrobial can be prescribed to treat all infections

    false

  • 51

    Positive Selection

    To enable microbes to grow on agar with antibiotics

  • 52

    Which mutagen results in pyrimidine dimers?

    UV Radiation

  • 53

    Which of the following is considered a mechanism of resistance for the protein synthesis?

    Produce natural inhibition against the grus from binding

  • 54

    Select all the desired quorum sensing:

    Cells sensing the concentration of their population, Cells will not fluoresce until there is enough of them, Bacteria will continue to grow until there is enough of them to begin an invasion

  • 55

    In a laboratory exploring DNA structure and it’s aplications, scientists are studying the factors influencing the separation of the DNA strands. During experiments involving DNA strand for processes like repliation or analysis, cytosine (C) and guanine (G) are consistantly identified at key positions to their:

    Formation of two hydrogn bonds, easing the separation of DNA strands during the laboratory process

  • 56

    The use of lysosome during the preparation of cheese and wines is effective in reducing the number of

    bacteria

  • 57

    What is the goal of developing a safe bacterial control?

    Kill most bacteria without causing harm to humans

  • 58

    Which type of mutation results if part of a chromosomebreaks off and reattaches to a different chromosome?

    Gross

  • 59

    Why do we use heat instead of helicase, topoisomerase, etc in PCR?

    Too many enzymes meas too many factors we must accomodate fr

  • 60

    Why is it said that nucleic acid polymerization occurs 5’ to 3’?

    The #3 carbon is where the chain grows and #5 carbon is in “front”

  • 61

    In the Ames test, what is the purpose of positve selection?

    To enable microbes to grow on agar with Antibiotics

  • 62

    Both prokaryotic and eukaryotic cells have their transcription and translation happening simultaneously in the cytoplasm

    True

  • 63

    Where does most gene-regulation occur in prokaryotes?

    Transcriptional level

  • 64

    Organize the steps of PCR

    95 C to separate, 50 C to anneal, 72 C to extend

  • 65

    A medium that contains high salt to inhibit some type of organism is considered?

    Selective

  • 66

    Which of the following categories do the vast majority of pathogens fall into?

    Mesophiles

  • 67

    In the context of prokaryotic cells, how can restriction enzymes be analogously compared to a biological immune system response against bacteriophages?\

    Restriction enzymes recognize and destroy specific DNA sequences, similar to how theimmune system targets specific pathogens

  • 68

    By ho much increase in atmosphericpressure (atm) if you were to dive 30 meters?

    3

  • 69

    The primary reason environmental conditions play a role in microbial growth is because these conditons affect____

    Activity and folding of enzymes and proteins

  • 70

    What Method would you use to quantify cells that are sparse in a solution?

    Filtration

  • 71

    Which of the following is/are involved in translation. Choose all that apply

    tRNA, Amino Acids, mRNA, rRNA

  • 72

    Which of the following would not be used during DNA fingering (gene cloning) procedure?

    Fluorescent Nucleotides

  • 73

    Where does most gene regulation occur in prokaryotes?

    Transcriptional Level

  • 74

    List the steps in order:

    Lag phase, Exponential Phase, Stationary Phase, Death Phase

  • 75

    The thermal death time for this culture under this condition is how many minutes?

    30 minutes

  • 76

    In Griffith's experiment on bacterial transformation, what crucial discovery indicated that a bacterial plasmid could turn harmless bacteria into deadly strains?

    The transformation of non-pathogenic bacteria into pathogenic ones in the presence of a heat-killed pathogenic strain

  • 77

    For disinfectant #3 the MIC is:

    0.5 ug/mL

  • 78

    When an insertion or detection of one or two nucleotides occurs in a coding region for a protein this type of mutation occurs

    Frameshift

  • 79

    In order for DNA to be copied, this must first be attached to the template strands at an origin of replication:

    Primer

  • 80

    Why is Taq polymerase unique?

    Survive extreme low and high temperature

  • 81

    The RNA type which brings amino acids to a ribosome is

    tRNA

  • 82

    What accidental discovery led alexander fleming’s penicillin breakthrough?

    Contamination in a petri dish

  • 83

    The theraputic drug index refers to:

    The concentration or dosing that can be tolerated

  • 84

    qPCR or sequencing

    Verify the codes of the gene sequence

  • 85

    What is the mechanism where opportunistic bacteria communicate with each other prior to an all-out invasion?

    Quorum sensing

  • 86

    The energy required for DNA replication comes from the:

    Triphosphate deoxyribonucleotide

  • 87

    Where specifically in a eukaryotic cell does the process of translation occur

    Rough ER

  • 88

    Chromosomal and plasmid DNA replication bubbles begin

    At the origin of replication

  • 89

    Which enzyme can proofread DNA during replication?

    DNA Polymerase III

  • 90

    What is the primary function of base-excision repai ad mismatch repair mechanism?

    replace incorrect bases

  • 91

    What are some examples of mechanisms of resistance?

    Utilize different metabolic pathways, Produce enzyme to breakdown drugs, Synthesize biofilm, Block drug entry

  • 92

    To minimize the development of resistance, it is advised to:

    use a combination of drugs

  • 93

    Transcription starts at a region of DNA called the

    Promoter

  • 94

    The RNA polymerase will constituitively bind and epress the regulatory gene?

    True

  • 95

    All food and products must pass FDA regulation before selling to consumers to ensure:

    They are safe for consumption

  • 96

    Which condition would prevent the trp operon from being transcribed?

    High levels of tryptophan

  • 97

    Why is the DNA synthesis of the lagging strand considered discontinuous?

    Synthesis is moving in the opposite direction from te replication fork

  • 98

    The trm cDNA refers to

    DNA tha is complementary to template RNA

  • 99

    If a researcher sets up a PCR reaction, but mistakenly uses a heat-sensitive polymerase, not a Taq polymerase, what will happen?

    The PCR will stop after one cycle

  • 100

    From the list below, which is the most resistant to methods of deactvation/killing?

    prions