問題一覧
1
tissue appear swollen and soapy
Molliflex
2
tissue will undergo complete digestion within 24- 48 hours.
55 c
3
Immersing in saturated Li2CO3 or 5-10% NaHCO3
POST DECALCIFICATION
4
fixative and decalcifying agent, best general decalcifying agent
5% FORMIC ACID
5
Fixative – decalcifying agent
CHROMIC ACID/ FLEMMINGS FLUID
6
The ideal time required for decalcifying tissue is
24-48 hours.
7
(+) charged calcium ions move towards the negative electrode using an electric charge, Shorter time of calcium removal
ELECTROPHORESIS
8
Hastens decalcification by using formic acid containing decalcifying solutions. Not recommended for fluids that contain mineral acids such as nitric acid or HCL
ION EXCHANGE RESINS
9
most rapid
Phloroglucin nitric acid
10
Von Ebner’s – small pieces, surface decalcification
HCL
11
Optimum temperature
ROOM TEMP (18-30 C)
12
Sequestration of calcium, Commonly used is EDTA (versene)
CHELATING AGENTS
13
Removal of calcium or lime salts from bones and calcified tissue
DECALCIFICATION
14
calcium oxalate
Chemical method
15
recommended ratio of fluid to tissue volume for decalcification is
20:1
16
most ideal, most sensitive, most reliable but very expensive
Xray method
17
tissue softener & decalcifying agent
Perenyi’s fluid
18
Dense bone tissues usually require up to
14 days or longer
19
impaired nuclear staining of Van Gieson’s stain for collagen fibers.
37 c
20
(5-10%): most common
NITRIC ACID