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暗記メーカー

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decalcification

decalcification
20問 • 2年前
  • Kmyching
    • 通報

    問題一覧

  • 1

    Removal of calcium or lime salts from bones and calcified tissue

    DECALCIFICATION

  • 2

    recommended ratio of fluid to tissue volume for decalcification is

    20:1

  • 3

    impaired nuclear staining of Van Gieson’s stain for collagen fibers.

    37 c

  • 4

    tissue will undergo complete digestion within 24- 48 hours.

    55 c

  • 5

    Optimum temperature

    ROOM TEMP (18-30 C)

  • 6

    The ideal time required for decalcifying tissue is

    24-48 hours.

  • 7

    Dense bone tissues usually require up to

    14 days or longer

  • 8

    (5-10%): most common

    NITRIC ACID

  • 9

    tissue softener & decalcifying agent

    Perenyi’s fluid

  • 10

    most rapid

    Phloroglucin nitric acid

  • 11

    Von Ebner’s – small pieces, surface decalcification

    HCL

  • 12

    fixative and decalcifying agent, best general decalcifying agent

    5% FORMIC ACID

  • 13

    Fixative – decalcifying agent

    CHROMIC ACID/ FLEMMINGS FLUID

  • 14

    Sequestration of calcium, Commonly used is EDTA (versene)

    CHELATING AGENTS

  • 15

    Hastens decalcification by using formic acid containing decalcifying solutions. Not recommended for fluids that contain mineral acids such as nitric acid or HCL

    ION EXCHANGE RESINS

  • 16

    (+) charged calcium ions move towards the negative electrode using an electric charge, Shorter time of calcium removal

    ELECTROPHORESIS

  • 17

    most ideal, most sensitive, most reliable but very expensive

    Xray method

  • 18

    calcium oxalate

    Chemical method

  • 19

    Immersing in saturated Li2CO3 or 5-10% NaHCO3

    POST DECALCIFICATION

  • 20

    tissue appear swollen and soapy

    Molliflex

    • HISTOPATH (lesson 1)

    HISTOPATH (lesson 1)

    Kmyching · 56問 · 2年前

    HISTOPATH (lesson 1)

    HISTOPATH (lesson 1)

    56問 • 2年前
    Kmyching

    bacte (microbial control)

    bacte (microbial control)

    Kmyching · 26問 · 2年前

    bacte (microbial control)

    bacte (microbial control)

    26問 • 2年前
    Kmyching

    Collection

    Collection

    Kmyching · 6問 · 2年前

    Collection

    Collection

    6問 • 2年前
    Kmyching

    Histopath (somatic death)

    Histopath (somatic death)

    Kmyching · 25問 · 2年前

    Histopath (somatic death)

    Histopath (somatic death)

    25問 • 2年前
    Kmyching

    fresh tissue

    fresh tissue

    Kmyching · 54問 · 2年前

    fresh tissue

    fresh tissue

    54問 • 2年前
    Kmyching

    Staphylococcus

    Staphylococcus

    Kmyching · 7問 · 2年前

    Staphylococcus

    Staphylococcus

    7問 • 2年前
    Kmyching

    lesson 1 (cell injury, adaptation and inflammation and repair)

    lesson 1 (cell injury, adaptation and inflammation and repair)

    Kmyching · 67問 · 2年前

    lesson 1 (cell injury, adaptation and inflammation and repair)

    lesson 1 (cell injury, adaptation and inflammation and repair)

    67問 • 2年前
    Kmyching

    biopsy

    biopsy

    Kmyching · 14問 · 2年前

    biopsy

    biopsy

    14問 • 2年前
    Kmyching

    autopsy

    autopsy

    Kmyching · 20問 · 2年前

    autopsy

    autopsy

    20問 • 2年前
    Kmyching

    DEHYDRATION AND CLEARING

    DEHYDRATION AND CLEARING

    Kmyching · 27問 · 2年前

    DEHYDRATION AND CLEARING

    DEHYDRATION AND CLEARING

    27問 • 2年前
    Kmyching

    IMPREGNATION AND EMBEDDING

    IMPREGNATION AND EMBEDDING

    Kmyching · 21問 · 2年前

    IMPREGNATION AND EMBEDDING

    IMPREGNATION AND EMBEDDING

    21問 • 2年前
    Kmyching

    TRIMMING AND SECTIONING

    TRIMMING AND SECTIONING

    Kmyching · 35問 · 2年前

    TRIMMING AND SECTIONING

    TRIMMING AND SECTIONING

    35問 • 2年前
    Kmyching

    adhesive and staining

    adhesive and staining

    Kmyching · 43問 · 2年前

    adhesive and staining

    adhesive and staining

    43問 • 2年前
    Kmyching

    COMMON STAINING SOLUTION

    COMMON STAINING SOLUTION

    Kmyching · 19問 · 2年前

    COMMON STAINING SOLUTION

    COMMON STAINING SOLUTION

    19問 • 2年前
    Kmyching

    OTHER STAINING

    OTHER STAINING

    Kmyching · 26問 · 2年前

    OTHER STAINING

    OTHER STAINING

    26問 • 2年前
    Kmyching

    H&E STAINING, MOUNTING, RINGING AND LABELLING

    H&E STAINING, MOUNTING, RINGING AND LABELLING

    Kmyching · 19問 · 1年前

    H&E STAINING, MOUNTING, RINGING AND LABELLING

    H&E STAINING, MOUNTING, RINGING AND LABELLING

    19問 • 1年前
    Kmyching

    IMMUNOHISTOCHEMISTRY

    IMMUNOHISTOCHEMISTRY

    Kmyching · 11問 · 1年前

    IMMUNOHISTOCHEMISTRY

    IMMUNOHISTOCHEMISTRY

    11問 • 1年前
    Kmyching

    EPITHELIAL TUMOR MARKERS

    EPITHELIAL TUMOR MARKERS

    Kmyching · 5問 · 1年前

    EPITHELIAL TUMOR MARKERS

    EPITHELIAL TUMOR MARKERS

    5問 • 1年前
    Kmyching

    INTERMEDIATE FILAMENT MARKERS

    INTERMEDIATE FILAMENT MARKERS

    Kmyching · 6問 · 1年前

    INTERMEDIATE FILAMENT MARKERS

    INTERMEDIATE FILAMENT MARKERS

    6問 • 1年前
    Kmyching

    NEUROENDOCRINE MARKERS and GERM CELL TUMOR MARKERS

    NEUROENDOCRINE MARKERS and GERM CELL TUMOR MARKERS

    Kmyching · 6問 · 1年前

    NEUROENDOCRINE MARKERS and GERM CELL TUMOR MARKERS

    NEUROENDOCRINE MARKERS and GERM CELL TUMOR MARKERS

    6問 • 1年前
    Kmyching

    MESENCHYMAL TUMOR MARKERS

    MESENCHYMAL TUMOR MARKERS

    Kmyching · 5問 · 1年前

    MESENCHYMAL TUMOR MARKERS

    MESENCHYMAL TUMOR MARKERS

    5問 • 1年前
    Kmyching

    問題一覧

  • 1

    Removal of calcium or lime salts from bones and calcified tissue

    DECALCIFICATION

  • 2

    recommended ratio of fluid to tissue volume for decalcification is

    20:1

  • 3

    impaired nuclear staining of Van Gieson’s stain for collagen fibers.

    37 c

  • 4

    tissue will undergo complete digestion within 24- 48 hours.

    55 c

  • 5

    Optimum temperature

    ROOM TEMP (18-30 C)

  • 6

    The ideal time required for decalcifying tissue is

    24-48 hours.

  • 7

    Dense bone tissues usually require up to

    14 days or longer

  • 8

    (5-10%): most common

    NITRIC ACID

  • 9

    tissue softener & decalcifying agent

    Perenyi’s fluid

  • 10

    most rapid

    Phloroglucin nitric acid

  • 11

    Von Ebner’s – small pieces, surface decalcification

    HCL

  • 12

    fixative and decalcifying agent, best general decalcifying agent

    5% FORMIC ACID

  • 13

    Fixative – decalcifying agent

    CHROMIC ACID/ FLEMMINGS FLUID

  • 14

    Sequestration of calcium, Commonly used is EDTA (versene)

    CHELATING AGENTS

  • 15

    Hastens decalcification by using formic acid containing decalcifying solutions. Not recommended for fluids that contain mineral acids such as nitric acid or HCL

    ION EXCHANGE RESINS

  • 16

    (+) charged calcium ions move towards the negative electrode using an electric charge, Shorter time of calcium removal

    ELECTROPHORESIS

  • 17

    most ideal, most sensitive, most reliable but very expensive

    Xray method

  • 18

    calcium oxalate

    Chemical method

  • 19

    Immersing in saturated Li2CO3 or 5-10% NaHCO3

    POST DECALCIFICATION

  • 20

    tissue appear swollen and soapy

    Molliflex