問題一覧
1
Removal of calcium or lime salts from bones and calcified tissue
DECALCIFICATION
2
recommended ratio of fluid to tissue volume for decalcification is
20:1
3
impaired nuclear staining of Van Gieson’s stain for collagen fibers.
37 c
4
tissue will undergo complete digestion within 24- 48 hours.
55 c
5
Optimum temperature
ROOM TEMP (18-30 C)
6
The ideal time required for decalcifying tissue is
24-48 hours.
7
Dense bone tissues usually require up to
14 days or longer
8
(5-10%): most common
NITRIC ACID
9
tissue softener & decalcifying agent
Perenyi’s fluid
10
most rapid
Phloroglucin nitric acid
11
Von Ebner’s – small pieces, surface decalcification
HCL
12
fixative and decalcifying agent, best general decalcifying agent
5% FORMIC ACID
13
Fixative – decalcifying agent
CHROMIC ACID/ FLEMMINGS FLUID
14
Sequestration of calcium, Commonly used is EDTA (versene)
CHELATING AGENTS
15
Hastens decalcification by using formic acid containing decalcifying solutions. Not recommended for fluids that contain mineral acids such as nitric acid or HCL
ION EXCHANGE RESINS
16
(+) charged calcium ions move towards the negative electrode using an electric charge, Shorter time of calcium removal
ELECTROPHORESIS
17
most ideal, most sensitive, most reliable but very expensive
Xray method
18
calcium oxalate
Chemical method
19
Immersing in saturated Li2CO3 or 5-10% NaHCO3
POST DECALCIFICATION
20
tissue appear swollen and soapy
Molliflex