問題一覧
1
It is a blood test measures amount and sites of your red blood cells, hemoglobin, white blood cells, and platelets.
Complete Blood Count
2
Components of CBC
WBC, RBC, Reticulocyte Count, Platelets, Hemoglobin, Hematocrit, RBC indices
3
Most common anticoagulant for routine testing
EDTA
4
Specimens for CBC should be analyzed within ____ at room temperature
6 hours
5
It is acounting chamber originally designed and usually used for counting blood cells
Hemacytometer
6
The most common counting chambee
Levy chamber with Improved Neubauer
7
The most commonly used counting chambee
Open type Spencer Brightine , Improved Neubauer
8
Touching top or right = ?
Do not count
9
Touching bottom or left
Do count
10
Each ruled area measures _____ divided into 9 large squares
3 sq mm
11
Each large square measure ____
1 sq mm
12
The central large area is divided into _______
25 intermediate squares
13
Each intermediate square measures _____
0.04 sq mm
14
RBC are counted in the ______ and _____ intermediate squares
Central and four corner
15
WBC are counted in the ______ square
Four corner large
16
Each large square is divided into ______ squares
16 smaller
17
Depth of the counting chamber
0.1 mm
18
is shaking the pipette technical/human error or standard/equipment errors
Technical or Human Error
19
is chipped or broken pipettes technical/human error or standard/equipment errors?
Standard or Equipment Errors
20
Diluting Fluid for Red Blood Cell
Isotonic Saline
21
Dilution Factor of Res Blood Cell
1:100
22
The red blood cell can be viewed under ___
40x
23
Does red blood cell has red bead or white bead
Red bead
24
Diluting fluid used in white blood cell count
1% ammonium oxalate or 3% acetic acid
25
white blood cell can be viewed under
10x
26
Area counted in white blood cell count for 1:100 dilution
9 mm2
27
Area counted in white blood cell count for 1:20 dilution
4 mm2
28
Diluting fluid for platelet count
1% ammonium oxalate
29
dilution factor for platelet count
1:100
30
Platelet count can be viewed under
40x
31
Area counted in platelet count
1 mm2
32
It is a red-iron bearing protein
Hemoglobin
33
Composed of four hemomokecukes which are conjugated with 2 pairs of globin chains
Hemoglobin
34
Molecular weight of hemoglobin
64,500 daltons
35
Methods for hemoglobin determination
Copper Sulfate / Specific Gravity Method, Gasometric Method, Chemical Method, Colorimetric Method
36
Method of choice for hemoglobin determination
Cyanmethemoglobin methods
37
In cyanmethemoglobin methods, test read photometrically at a wavelength of __
540 nm
38
The presence of free hemoglobin in the blood plasma
Hemoglobinemia
39
Normal values for men
14.0 - 18.0 g/dL
40
Normal values for women
12.0 - 16.0 g/dL
41
Normal values in late pregnancy
8.5 - 14.0 g/dL
42
Normal values for children
14.0 - 26.0 g/dL
43
Volume of packed RBCs that occupies a given volume of whole blood
Hematocrit
44
Hematocrit often referred to as
Packed cell volume
45
Used in evaluating and classifying varioisntyoes of anemia according to red cell indices
Hematocrit
46
Types of Hematocrit reading
Micro-hematocrit method, Micro-hematocrit using readcrit centrifuge, Macro-hematocrit method, Automated method
47
the micro-hematocrit using readcrit centrifuge uses a _____ which eliminates the use of sealants
Sureprep capillary tubes
48
The macro-hematocrit method uses what type of tube?
Wintrobe hematocrit tube
49
Used as quick visual check of the results of the hemoglobin and hematocrit
Rule of three
50
The rule of three applies only to specimens that have
Normocytic and Normochromic RBCs
51
Blood films should be made within _____ of drawing the specimen
4 hours
52
After 5 hours at room temp causes unacceptable blood cell artifacts such as
Echinocytic red blood cells, Spherocytes, Necrobiotic leukocytes, Vacuolated neutrophils
53
Most convinient and most commonly used method for smear preparation
Manual wedge techniques
54
Angle for Manual Wedge Techniques
30 to 45’
55
Too large drop
creates a long or thick film
56
too small drop
makes a short or thin blood films
57
Types of stains used in PBS and BM smear
Romanowsky stain, Pure wright stain, Wright-Giemsa stain
58
Methylene blue is ____ and stains ____
Basic and Acid
59
Eosin is ____ and stains _____
Acid and Basic
60
Types of staining method
Manual technique, Automated slide strainers, Quick stains
61
In macroscopic, a well-stained blood film should be in what color?
Pink to purple
62
In microscopic, RBCs should appear
Orange to salmon pink
63
In microscope, WBC nuclei should be
Purple to blue
64
In microscope, cytoplasma of neutrophil should be
Tan to pink with violet or lilac granules
65
In microscope, eosinophils should have ____ refractile granules
Bright orange
66
Track pattern performed for differential count
Back-and-forth serpentine and battlement